PyroTyping, a novel pyrosequencing-based assay for Mycobacterium tuberculosis genotyping

B. Molina-Moya; A. Lacoma; N. García-Sierra; S. Blanco; L. Haba; S. Samper; J. Ruiz-Manzano; C. Prat; C. Arnold; J. Domínguez

doi:10.1038/s41598-017-06760-5

PyroTyping, a novel pyrosequencing-based assay for Mycobacterium tuberculosis genotyping

B. Molina-Moya, A. Lacoma, N. García-Sierra, S. Blanco, L. Haba, S. Samper, J. Ruiz-Manzano, C. Prat, C. Arnold, J. Domínguez^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

We developed a novel method, PyroTyping, for discrimination of Mycobacterium tuberculosis isolates combining pyrosequencing and IS6110 polymorphism. A total of 100 isolates were analysed with IS6110-restriction fragment length polymorphism (RFLP), spoligotyping, mycobacterial interspersed repetitive units - variable number tandem repeats (MIRU-VNTR), and PyroTyping. PyroTyping results regarding clustering or discrimination of the isolates were highly concordant with the other typing methods performed. PyroTyping is more rapid than RFLP and presents the same discriminatory power, thus, it may be useful for taking timely decisions for tuberculosis control.

Original language	English
Article number	6777
Journal	Scientific Reports
Volume	7
Issue number	1
DOIs	https://doi.org/10.1038/s41598-017-06760-5
Publication status	Published - 1 Dec 2017

Bibliographical note

Publisher Copyright:
© 2017 The Author(s).

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abstract = "We developed a novel method, PyroTyping, for discrimination of Mycobacterium tuberculosis isolates combining pyrosequencing and IS6110 polymorphism. A total of 100 isolates were analysed with IS6110-restriction fragment length polymorphism (RFLP), spoligotyping, mycobacterial interspersed repetitive units - variable number tandem repeats (MIRU-VNTR), and PyroTyping. PyroTyping results regarding clustering or discrimination of the isolates were highly concordant with the other typing methods performed. PyroTyping is more rapid than RFLP and presents the same discriminatory power, thus, it may be useful for taking timely decisions for tuberculosis control.",

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AU - Molina-Moya, B.

AU - Lacoma, A.

AU - García-Sierra, N.

AU - Blanco, S.

AU - Haba, L.

AU - Samper, S.

AU - Ruiz-Manzano, J.

AU - Prat, C.

AU - Arnold, C.

AU - Domínguez, J.

PY - 2017/12/1

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N2 - We developed a novel method, PyroTyping, for discrimination of Mycobacterium tuberculosis isolates combining pyrosequencing and IS6110 polymorphism. A total of 100 isolates were analysed with IS6110-restriction fragment length polymorphism (RFLP), spoligotyping, mycobacterial interspersed repetitive units - variable number tandem repeats (MIRU-VNTR), and PyroTyping. PyroTyping results regarding clustering or discrimination of the isolates were highly concordant with the other typing methods performed. PyroTyping is more rapid than RFLP and presents the same discriminatory power, thus, it may be useful for taking timely decisions for tuberculosis control.

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PyroTyping, a novel pyrosequencing-based assay for Mycobacterium tuberculosis genotyping

Abstract

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