We developed a novel method, PyroTyping, for discrimination of Mycobacterium tuberculosis isolates combining pyrosequencing and IS6110 polymorphism. A total of 100 isolates were analysed with IS6110-restriction fragment length polymorphism (RFLP), spoligotyping, mycobacterial interspersed repetitive units - variable number tandem repeats (MIRU-VNTR), and PyroTyping. PyroTyping results regarding clustering or discrimination of the isolates were highly concordant with the other typing methods performed. PyroTyping is more rapid than RFLP and presents the same discriminatory power, thus, it may be useful for taking timely decisions for tuberculosis control.
Bibliographical noteFunding Information:
Thanks to all members involved in Unitats de Vigil?ncia Epidemiol?gica, Departament de Salut, Generalitat de Catalunya. Special thanks to Oriol Martos for his invaluable technical assistance. Authors would like to acknowledge the use of Servicio General de Apoyo a la Investigaci?n-SAI, Universidad de Zaragoza. JD is funded by the Miguel Servet program of the Instituto de Salud Carlos III (Spain). NG was recipient of a pre-doctoral grant (2009FI-B-0134) from Generalitat de Catalunya. The research was supported by grants from the Instituto de Salud Carlos III (PI07/0551, 15/0317, and 16/01912), integrated in the Plan Nacional de I+D+I and cofunded by the ISCIII-Subdirecci?n General de Evaluaci?n and the Fondo Europeo de Desarrollo Regional (FEDER), from the Sociedad Espa?ola de Neumolog?a y Cirug?a Tor?cica (SEPAR) (Barcelona, Spain); a short-stage grant from Sociedad Espa?ola de Enfermedades Infecciosas y Microbiolog?a Cl?nica (SEIMC); and from CIBERES in the First call of technology transference research projects. The funders had no role in the study design, data collection and interpretation, or the decision to submit the work for publication.