TY - JOUR
T1 - Pyrosequencing analysis of 20 nucleotides of internal transcribed spacer 2 discriminates Candida parapsilosis, Candida metapsilosis, and Candida orthopsilosis
AU - Borman, Andrew M.
AU - Linton, Christopher J.
AU - Oliver, Debra
AU - Palmer, Michael D.
AU - Szekely, Adrien
AU - Odds, Frank C.
AU - Johnson, Elizabeth M.
PY - 2009/7
Y1 - 2009/7
N2 - Two new cryptic sister species, Candida orthopsilosis and Candida metapsilosis, were recently identified by consistent DNA sequence differences among several genes within the genetically heterogeneous Candida parapsilosis complex. Here, we present data demonstrating that Pyrosequencing analysis of 20 nucleotides of internal transcribed spacer region 2 rapidly and robustly distinguishes between these three closely related Candida species.
AB - Two new cryptic sister species, Candida orthopsilosis and Candida metapsilosis, were recently identified by consistent DNA sequence differences among several genes within the genetically heterogeneous Candida parapsilosis complex. Here, we present data demonstrating that Pyrosequencing analysis of 20 nucleotides of internal transcribed spacer region 2 rapidly and robustly distinguishes between these three closely related Candida species.
UR - http://www.scopus.com/inward/record.url?scp=67650079041&partnerID=8YFLogxK
U2 - 10.1128/JCM.00240-09
DO - 10.1128/JCM.00240-09
M3 - Article
C2 - 19403763
AN - SCOPUS:67650079041
SN - 0095-1137
VL - 47
SP - 2307
EP - 2310
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 7
ER -