Protective mucosal immunity elicited by targeted iliac lymph node immunization with a subunit SIV envelope and core vaccine in macaques

Thomas Lehner; Yufei Wang; Martin Cranage; Lesley A. Bergmeier; Elaine Mitchell; Louisa Tao; Graham Hall; Michael Dennis; Nicola Cook; Roger Brookes; Linda Klavinskis; Ian Jones; Carl Doyle; Robert Ward

doi:10.1038/nm0796-767

Protective mucosal immunity elicited by targeted iliac lymph node immunization with a subunit SIV envelope and core vaccine in macaques

Thomas Lehner^*, Yufei Wang, Martin Cranage, Lesley A. Bergmeier, Elaine Mitchell, Louisa Tao, Graham Hall, Michael Dennis, Nicola Cook, Roger Brookes, Linda Klavinskis, Ian Jones, Carl Doyle, Robert Ward

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Abstract

Prevention of sexually transmitted HIV infection was investigated in macaques by immunization with a recombinant SIV (simian immunodeficiency virus) envelope gp120 and core p27 vaccine. In two independent series of experiments, we used the novel targeted iliac lymph node (TILN) route of immunization, aiming close to the iliac lymph nodes draining the genitorectal mucosa. Rectal challenge with the SIVmac 32H J5 molecular clone in two series induced total protection in four out of seven macaques immunized by TILN, compared with infection in 13 of 14 unimmunized macaques or immunized by other routes (P = 0.025). The remaining three macaques showed either a decrease in viral load (> 90%) or transient viremia, indicating that ail seven TILN-immunized macaques showed total or partial protection (P = 0.001). Protection was associated with significant increase in the iliac lymph nodes of IgA antibody-secreting cells to p27 (P < 0.02), CD8-suppressor factor (P < 0.01), and the chemokines RANTES and MIP-1β (P < 0.01).

Original language	English
Pages (from-to)	767-775
Number of pages	9
Journal	Nature Medicine
Volume	2
Issue number	7
DOIs	https://doi.org/10.1038/nm0796-767
Publication status	Published - Jul 1996

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Lehner, T., Wang, Y., Cranage, M., Bergmeier, L. A., Mitchell, E., Tao, L., Hall, G., Dennis, M., Cook, N., Brookes, R., Klavinskis, L., Jones, I., Doyle, C., & Ward, R. (1996). Protective mucosal immunity elicited by targeted iliac lymph node immunization with a subunit SIV envelope and core vaccine in macaques. Nature Medicine, 2(7), 767-775. https://doi.org/10.1038/nm0796-767

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title = "Protective mucosal immunity elicited by targeted iliac lymph node immunization with a subunit SIV envelope and core vaccine in macaques",

abstract = "Prevention of sexually transmitted HIV infection was investigated in macaques by immunization with a recombinant SIV (simian immunodeficiency virus) envelope gp120 and core p27 vaccine. In two independent series of experiments, we used the novel targeted iliac lymph node (TILN) route of immunization, aiming close to the iliac lymph nodes draining the genitorectal mucosa. Rectal challenge with the SIVmac 32H J5 molecular clone in two series induced total protection in four out of seven macaques immunized by TILN, compared with infection in 13 of 14 unimmunized macaques or immunized by other routes (P = 0.025). The remaining three macaques showed either a decrease in viral load (> 90%) or transient viremia, indicating that ail seven TILN-immunized macaques showed total or partial protection (P = 0.001). Protection was associated with significant increase in the iliac lymph nodes of IgA antibody-secreting cells to p27 (P < 0.02), CD8-suppressor factor (P < 0.01), and the chemokines RANTES and MIP-1β (P < 0.01).",

author = "Thomas Lehner and Yufei Wang and Martin Cranage and Bergmeier, {Lesley A.} and Elaine Mitchell and Louisa Tao and Graham Hall and Michael Dennis and Nicola Cook and Roger Brookes and Linda Klavinskis and Ian Jones and Carl Doyle and Robert Ward",

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Lehner, T, Wang, Y, Cranage, M, Bergmeier, LA, Mitchell, E, Tao, L, Hall, G, Dennis, M, Cook, N, Brookes, R, Klavinskis, L, Jones, I, Doyle, C & Ward, R 1996, 'Protective mucosal immunity elicited by targeted iliac lymph node immunization with a subunit SIV envelope and core vaccine in macaques', Nature Medicine, vol. 2, no. 7, pp. 767-775. https://doi.org/10.1038/nm0796-767

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AU - Lehner, Thomas

AU - Wang, Yufei

AU - Cranage, Martin

AU - Bergmeier, Lesley A.

AU - Mitchell, Elaine

AU - Tao, Louisa

AU - Hall, Graham

AU - Dennis, Michael

AU - Cook, Nicola

AU - Brookes, Roger

AU - Klavinskis, Linda

AU - Jones, Ian

AU - Doyle, Carl

AU - Ward, Robert

PY - 1996/7

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N2 - Prevention of sexually transmitted HIV infection was investigated in macaques by immunization with a recombinant SIV (simian immunodeficiency virus) envelope gp120 and core p27 vaccine. In two independent series of experiments, we used the novel targeted iliac lymph node (TILN) route of immunization, aiming close to the iliac lymph nodes draining the genitorectal mucosa. Rectal challenge with the SIVmac 32H J5 molecular clone in two series induced total protection in four out of seven macaques immunized by TILN, compared with infection in 13 of 14 unimmunized macaques or immunized by other routes (P = 0.025). The remaining three macaques showed either a decrease in viral load (> 90%) or transient viremia, indicating that ail seven TILN-immunized macaques showed total or partial protection (P = 0.001). Protection was associated with significant increase in the iliac lymph nodes of IgA antibody-secreting cells to p27 (P < 0.02), CD8-suppressor factor (P < 0.01), and the chemokines RANTES and MIP-1β (P < 0.01).

AB - Prevention of sexually transmitted HIV infection was investigated in macaques by immunization with a recombinant SIV (simian immunodeficiency virus) envelope gp120 and core p27 vaccine. In two independent series of experiments, we used the novel targeted iliac lymph node (TILN) route of immunization, aiming close to the iliac lymph nodes draining the genitorectal mucosa. Rectal challenge with the SIVmac 32H J5 molecular clone in two series induced total protection in four out of seven macaques immunized by TILN, compared with infection in 13 of 14 unimmunized macaques or immunized by other routes (P = 0.025). The remaining three macaques showed either a decrease in viral load (> 90%) or transient viremia, indicating that ail seven TILN-immunized macaques showed total or partial protection (P = 0.001). Protection was associated with significant increase in the iliac lymph nodes of IgA antibody-secreting cells to p27 (P < 0.02), CD8-suppressor factor (P < 0.01), and the chemokines RANTES and MIP-1β (P < 0.01).

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Protective mucosal immunity elicited by targeted iliac lymph node immunization with a subunit SIV envelope and core vaccine in macaques

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