Protective mucosal immunity elicited by targeted iliac lymph node immunization with a subunit SIV envelope and core vaccine in macaques

Thomas Lehner*, Yufei Wang, Martin Cranage, Lesley A. Bergmeier, Elaine Mitchell, Louisa Tao, Graham Hall, Michael Dennis, Nicola Cook, Roger Brookes, Linda Klavinskis, Ian Jones, Carl Doyle, Robert Ward

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

273 Citations (Scopus)

Abstract

Prevention of sexually transmitted HIV infection was investigated in macaques by immunization with a recombinant SIV (simian immunodeficiency virus) envelope gp120 and core p27 vaccine. In two independent series of experiments, we used the novel targeted iliac lymph node (TILN) route of immunization, aiming close to the iliac lymph nodes draining the genitorectal mucosa. Rectal challenge with the SIVmac 32H J5 molecular clone in two series induced total protection in four out of seven macaques immunized by TILN, compared with infection in 13 of 14 unimmunized macaques or immunized by other routes (P = 0.025). The remaining three macaques showed either a decrease in viral load (> 90%) or transient viremia, indicating that ail seven TILN-immunized macaques showed total or partial protection (P = 0.001). Protection was associated with significant increase in the iliac lymph nodes of IgA antibody-secreting cells to p27 (P < 0.02), CD8-suppressor factor (P < 0.01), and the chemokines RANTES and MIP-1β (P < 0.01).

Original languageEnglish
Pages (from-to)767-775
Number of pages9
JournalNature Medicine
Volume2
Issue number7
DOIs
Publication statusPublished - Jul 1996

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