TY - JOUR
T1 - Prospective study of verocytotoxin–producing, enteroaggregative and diffusely adherent Escherichia coli in different diarrhoeal states
AU - Brook, M. G.
AU - Smith, H. R.
AU - Bannister, B. A.
AU - McConnell, M.
AU - Chart, H.
AU - Scotland, S. M.
AU - Sawyer, A.
AU - Smith, M.
AU - Rowe, B.
PY - 1994/2
Y1 - 1994/2
N2 - One hundred and eighty–one stool specimens from patients with various types of diarrhoea (135 patients) or from non-diarrhoeal controls (23 acute medical patients, 23 inflammatory bowel disease in remission) were investigated using a colony–blot DNA hybridization assay for the presence of Verocytotoxin–producing (VTEC), enteroaggregative (EAggEC) and diffusely adherent (DAEC) Escherichia coli. Twelve patients had probe–positive EAggEC in the stool and 8 of these had diarrhoea, 6 following recent travel. Eight patients had DAEC, 7 of whom had travellers diarrhoea. Six of 10 (60%) travellers with gastroenteritis, but without a recognized enteric pathogen, were positive for EAggEC (4) or DAEC (2). Five of 10 (50%) travellers with gastroenteritis related to a recognized enteric pathogen also had DAEC identified in their stool. Of the 23 acute medical control patients 11 had been abroad, 4 of these were immigrants and had EAggEC. VTEC were not found and, with one exception, immunoassays for antibodies to E. coli O 157 and O 2 lipopolysaccharides were negative.
AB - One hundred and eighty–one stool specimens from patients with various types of diarrhoea (135 patients) or from non-diarrhoeal controls (23 acute medical patients, 23 inflammatory bowel disease in remission) were investigated using a colony–blot DNA hybridization assay for the presence of Verocytotoxin–producing (VTEC), enteroaggregative (EAggEC) and diffusely adherent (DAEC) Escherichia coli. Twelve patients had probe–positive EAggEC in the stool and 8 of these had diarrhoea, 6 following recent travel. Eight patients had DAEC, 7 of whom had travellers diarrhoea. Six of 10 (60%) travellers with gastroenteritis, but without a recognized enteric pathogen, were positive for EAggEC (4) or DAEC (2). Five of 10 (50%) travellers with gastroenteritis related to a recognized enteric pathogen also had DAEC identified in their stool. Of the 23 acute medical control patients 11 had been abroad, 4 of these were immigrants and had EAggEC. VTEC were not found and, with one exception, immunoassays for antibodies to E. coli O 157 and O 2 lipopolysaccharides were negative.
UR - http://www.scopus.com/inward/record.url?scp=0028047854&partnerID=8YFLogxK
U2 - 10.1017/S0950268800057423
DO - 10.1017/S0950268800057423
M3 - Article
C2 - 8119365
AN - SCOPUS:0028047854
SN - 0950-2688
VL - 112
SP - 63
EP - 67
JO - Epidemiology and Infection
JF - Epidemiology and Infection
IS - 1
ER -
