Prospective evaluation of a rapid immunochromogenic cassette test for the diagnosis of melioidosis in northeast Thailand

Allen C. Cheng; Sharon J. Peacock; Direk Limmathurotsakul; Gumphol Wongsuvan; Wirongrong Chierakul; Premjit Amornchai; Nongluk Getchalarat; Wipada Chaowagul; Nicholas J. White; Nicholas P.J. Day; Vanaporn Wuthiekanun

doi:10.1016/j.trstmh.2005.04.019

Prospective evaluation of a rapid immunochromogenic cassette test for the diagnosis of melioidosis in northeast Thailand

Allen C. Cheng, Sharon J. Peacock, Direk Limmathurotsakul, Gumphol Wongsuvan, Wirongrong Chierakul, Premjit Amornchai, Nongluk Getchalarat, Wipada Chaowagul, Nicholas J. White, Nicholas P.J. Day, Vanaporn Wuthiekanun^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

A prospective study was performed to compare a rapid immunochromogenic cassette test (ICT) with the indirect haemagglutination assay (IHA) and clinical rules for the diagnosis of melioidosis in an endemic area. The sensitivity and specificity of the IgG ICT was 86% and 47%, and the IgM ICT was 82% and 47%, respectively. These were similar to the results for IHA (sensitivity 73%, specificity 64%) and clinical rules (73% and 37%). ICT lacks clinical utility as a result of high background rates of positive Burkholderia pseudomallei serology in this population. Low sensitivity and specificity of clinical rules is consistent with the protean manifestations of melioidosis and clinical difficulty in identifying patients with melioidosis.

Original language	English
Pages (from-to)	64-67
Number of pages	4
Journal	Transactions of the Royal Society of Tropical Medicine and Hygiene
Volume	100
Issue number	1
DOIs	https://doi.org/10.1016/j.trstmh.2005.04.019
Publication status	Published - Jan 2006
Externally published	Yes

Bibliographical note

Funding Information:
We are grateful for the support of the medical, nursing and laboratory staff at Sappasithiprasong Hospital and staff at the Wellcome Trust–Mahidol University–Oxford University Tropical Medicine Research Program, including Sayan Langa and Sukallaya Paengmee. We thank Denise Rowland and Janet Fox at PanBio Ltd for their support. A.C. is supported by an Australian National Health and Medical Council Training Scholarship and S.J.P. by a Wellcome Trust Career Development Award in Clinical Tropical Medicine. This study was funded by the Wellcome Trust of Great Britain. PanBio Ltd (Queensland, Australia) donated all test kits used in the study.

Keywords

Burkholderia pseudomallei
Immunochromogenic cassette test
Indirect haemagglutination assay
Melioidosis
Thailand

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.trstmh.2005.04.019

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Cheng, A. C., Peacock, S. J., Limmathurotsakul, D., Wongsuvan, G., Chierakul, W., Amornchai, P., Getchalarat, N., Chaowagul, W., White, N. J., Day, N. P. J., & Wuthiekanun, V. (2006). Prospective evaluation of a rapid immunochromogenic cassette test for the diagnosis of melioidosis in northeast Thailand. Transactions of the Royal Society of Tropical Medicine and Hygiene, 100(1), 64-67. https://doi.org/10.1016/j.trstmh.2005.04.019

@article{46130acaaac9456aa7589e1534901bf7,

title = "Prospective evaluation of a rapid immunochromogenic cassette test for the diagnosis of melioidosis in northeast Thailand",

abstract = "A prospective study was performed to compare a rapid immunochromogenic cassette test (ICT) with the indirect haemagglutination assay (IHA) and clinical rules for the diagnosis of melioidosis in an endemic area. The sensitivity and specificity of the IgG ICT was 86\% and 47\%, and the IgM ICT was 82\% and 47\%, respectively. These were similar to the results for IHA (sensitivity 73\%, specificity 64\%) and clinical rules (73\% and 37\%). ICT lacks clinical utility as a result of high background rates of positive Burkholderia pseudomallei serology in this population. Low sensitivity and specificity of clinical rules is consistent with the protean manifestations of melioidosis and clinical difficulty in identifying patients with melioidosis.",

keywords = "Burkholderia pseudomallei, Immunochromogenic cassette test, Indirect haemagglutination assay, Melioidosis, Thailand",

author = "Cheng, \{Allen C.\} and Peacock, \{Sharon J.\} and Direk Limmathurotsakul and Gumphol Wongsuvan and Wirongrong Chierakul and Premjit Amornchai and Nongluk Getchalarat and Wipada Chaowagul and White, \{Nicholas J.\} and Day, \{Nicholas P.J.\} and Vanaporn Wuthiekanun",

note = "Funding Information: We are grateful for the support of the medical, nursing and laboratory staff at Sappasithiprasong Hospital and staff at the Wellcome Trust–Mahidol University–Oxford University Tropical Medicine Research Program, including Sayan Langa and Sukallaya Paengmee. We thank Denise Rowland and Janet Fox at PanBio Ltd for their support. A.C. is supported by an Australian National Health and Medical Council Training Scholarship and S.J.P. by a Wellcome Trust Career Development Award in Clinical Tropical Medicine. This study was funded by the Wellcome Trust of Great Britain. PanBio Ltd (Queensland, Australia) donated all test kits used in the study.",

year = "2006",

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doi = "10.1016/j.trstmh.2005.04.019",

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Cheng, AC, Peacock, SJ, Limmathurotsakul, D, Wongsuvan, G, Chierakul, W, Amornchai, P, Getchalarat, N, Chaowagul, W, White, NJ, Day, NPJ & Wuthiekanun, V 2006, 'Prospective evaluation of a rapid immunochromogenic cassette test for the diagnosis of melioidosis in northeast Thailand', Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 100, no. 1, pp. 64-67. https://doi.org/10.1016/j.trstmh.2005.04.019

TY - JOUR

T1 - Prospective evaluation of a rapid immunochromogenic cassette test for the diagnosis of melioidosis in northeast Thailand

AU - Cheng, Allen C.

AU - Peacock, Sharon J.

AU - Limmathurotsakul, Direk

AU - Wongsuvan, Gumphol

AU - Chierakul, Wirongrong

AU - Amornchai, Premjit

AU - Getchalarat, Nongluk

AU - Chaowagul, Wipada

AU - White, Nicholas J.

AU - Day, Nicholas P.J.

AU - Wuthiekanun, Vanaporn

N1 - Funding Information: We are grateful for the support of the medical, nursing and laboratory staff at Sappasithiprasong Hospital and staff at the Wellcome Trust–Mahidol University–Oxford University Tropical Medicine Research Program, including Sayan Langa and Sukallaya Paengmee. We thank Denise Rowland and Janet Fox at PanBio Ltd for their support. A.C. is supported by an Australian National Health and Medical Council Training Scholarship and S.J.P. by a Wellcome Trust Career Development Award in Clinical Tropical Medicine. This study was funded by the Wellcome Trust of Great Britain. PanBio Ltd (Queensland, Australia) donated all test kits used in the study.

PY - 2006/1

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N2 - A prospective study was performed to compare a rapid immunochromogenic cassette test (ICT) with the indirect haemagglutination assay (IHA) and clinical rules for the diagnosis of melioidosis in an endemic area. The sensitivity and specificity of the IgG ICT was 86% and 47%, and the IgM ICT was 82% and 47%, respectively. These were similar to the results for IHA (sensitivity 73%, specificity 64%) and clinical rules (73% and 37%). ICT lacks clinical utility as a result of high background rates of positive Burkholderia pseudomallei serology in this population. Low sensitivity and specificity of clinical rules is consistent with the protean manifestations of melioidosis and clinical difficulty in identifying patients with melioidosis.

AB - A prospective study was performed to compare a rapid immunochromogenic cassette test (ICT) with the indirect haemagglutination assay (IHA) and clinical rules for the diagnosis of melioidosis in an endemic area. The sensitivity and specificity of the IgG ICT was 86% and 47%, and the IgM ICT was 82% and 47%, respectively. These were similar to the results for IHA (sensitivity 73%, specificity 64%) and clinical rules (73% and 37%). ICT lacks clinical utility as a result of high background rates of positive Burkholderia pseudomallei serology in this population. Low sensitivity and specificity of clinical rules is consistent with the protean manifestations of melioidosis and clinical difficulty in identifying patients with melioidosis.

KW - Burkholderia pseudomallei

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KW - Indirect haemagglutination assay

KW - Melioidosis

KW - Thailand

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AN - SCOPUS:28244439143

SN - 0035-9203

VL - 100

SP - 64

EP - 67

JO - Transactions of the Royal Society of Tropical Medicine and Hygiene

JF - Transactions of the Royal Society of Tropical Medicine and Hygiene

IS - 1

ER -

Prospective evaluation of a rapid immunochromogenic cassette test for the diagnosis of melioidosis in northeast Thailand

Abstract

Bibliographical note

Keywords

UN SDGs

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