Progress in the Microbiological Applications of Mass Spectrometry: from Electron Impact to Soft Ionization Techniques, MALDI-TOF MS and Beyond

Emmanuel Raptakis, Ajit J. Shah, Saheer E. Gharbia, Laila M.N. Shah, Simona Francese, Erika Y. Tranfield, Louise Duncan, Haroun N. Shah

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Early applications of mass spectrometry using hard ionization techniques targeted mainly bacterial lipids for studies on chemotaxonomy in the 1970–1980s. GC-MS emerged from this period for the analysis of long-chain cellular fatty acids for microbial identification until 2000. However, the arrival of soft ionization methods, such as MALDI-TOF MS, in the mid-1990s, with its negligible sample preparation, rapid analysis, low cost and development of comprehensive databases, superseded earlier methods and, in a decade, revolutionized clinical microbiology and provided a basis for applications into industry and the environment. New applications of the technology stimulated broader applications in MALDI imaging, and new instruments enabled bottom-up tandem MS/MS analysis to transition to top-down methods to gain more detailed insight into proteoforms. Protein dynamics and interactions are now being elucidated using methods such as HDX-MS while more versatile state-of-the-art MS instruments such as the Omnitrap are under development.

Original languageEnglish
Title of host publicationMicrobiological Identification Using MALDI-TOF and Tandem Mass Spectrometry
Subtitle of host publicationIndustrial and Environmental Applications
Publisherwiley
Pages1-44
Number of pages44
ISBN (Electronic)9781119814085
ISBN (Print)9781119814054
DOIs
Publication statusPublished - 1 Jan 2023

Bibliographical note

Publisher Copyright:
© 2023 John Wiley & Sons Ltd. All rights reserved.

Fingerprint

Dive into the research topics of 'Progress in the Microbiological Applications of Mass Spectrometry: from Electron Impact to Soft Ionization Techniques, MALDI-TOF MS and Beyond'. Together they form a unique fingerprint.

Cite this