Abstract
Early applications of mass spectrometry using hard ionization techniques targeted mainly bacterial lipids for studies on chemotaxonomy in the 1970–1980s. GC-MS emerged from this period for the analysis of long-chain cellular fatty acids for microbial identification until 2000. However, the arrival of soft ionization methods, such as MALDI-TOF MS, in the mid-1990s, with its negligible sample preparation, rapid analysis, low cost and development of comprehensive databases, superseded earlier methods and, in a decade, revolutionized clinical microbiology and provided a basis for applications into industry and the environment. New applications of the technology stimulated broader applications in MALDI imaging, and new instruments enabled bottom-up tandem MS/MS analysis to transition to top-down methods to gain more detailed insight into proteoforms. Protein dynamics and interactions are now being elucidated using methods such as HDX-MS while more versatile state-of-the-art MS instruments such as the Omnitrap are under development.
Original language | English |
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Title of host publication | Microbiological Identification Using MALDI-TOF and Tandem Mass Spectrometry |
Subtitle of host publication | Industrial and Environmental Applications |
Publisher | wiley |
Pages | 1-44 |
Number of pages | 44 |
ISBN (Electronic) | 9781119814085 |
ISBN (Print) | 9781119814054 |
DOIs | |
Publication status | Published - 1 Jan 2023 |
Bibliographical note
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