Abstract
We report the application of a covalent probe based on a D-glucosamine scaffold for the profiling of the bacterial pathogen Klebsiella pneumoniae. Incubation of K. pneumoniae lysates with the probe followed by electrophoretic separation and in-gel fluorescence detection allowed the generation of strain-specific signatures and the differentiation of a carbapenem-resistant strain. The labelling profile of the probe was independent of its anomeric configuration and included several low-abundance proteins not readily detectable by conventional protein staining. Initial target identification experiments by mass spectrometry suggest that target proteins include several carbohydrate-recognising proteins, which indicates that the sugar scaffold may have a role for target recognition.
Original language | English |
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Article number | 115900 |
Number of pages | 8 |
Journal | Bioorganic and Medicinal Chemistry |
Volume | 30 |
DOIs | |
Publication status | Published - 15 Jan 2021 |
Bibliographical note
Funding Information:We thank King's College London for a studentship (to CCM) and a King's China Award (to YX), the EPSRC National Mass Spectrometry Facility, Swansea, for the recording of mass spectra, and Dr Andrew Atkinson (King's College London) for recording the NMR spectra of 5-?. This work was supported by an EPSRC Impact Acceleration Award (EP/R511559/1).
Funding Information:
We thank King’s College London for a studentship (to CCM) and a King’s China Award (to YX), the EPSRC National Mass Spectrometry Facility, Swansea, for the recording of mass spectra, and Dr Andrew Atkinson (King’s College London) for recording the NMR spectra of 5-β . This work was supported by an EPSRC Impact Acceleration Award (EP/R511559/1).
Keywords
- Antimicrobial resistance
- Bacterial profiling
- Carbohydrate
- Covalent probe
- Klebsiella