Profiling protein expression in Klebsiella pneumoniae with a carbohydrate-based covalent probe

Camille C. Metier; Jiaming Peng; Yong Xu; Hayley Wootton; Victoire Riesi; Steven Lynham; Sirine Zadi; Carrie Turner; Matthew Wand; J. Mark Sutton; Gerd K. Wagner

doi:10.1016/j.bmc.2020.115900

Profiling protein expression in Klebsiella pneumoniae with a carbohydrate-based covalent probe

Camille C. Metier, Jiaming Peng, Yong Xu, Hayley Wootton, Victoire Riesi, Steven Lynham, Sirine Zadi, Carrie Turner, Matthew Wand, J. Mark Sutton, Gerd K. Wagner^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

We report the application of a covalent probe based on a D-glucosamine scaffold for the profiling of the bacterial pathogen Klebsiella pneumoniae. Incubation of K. pneumoniae lysates with the probe followed by electrophoretic separation and in-gel fluorescence detection allowed the generation of strain-specific signatures and the differentiation of a carbapenem-resistant strain. The labelling profile of the probe was independent of its anomeric configuration and included several low-abundance proteins not readily detectable by conventional protein staining. Initial target identification experiments by mass spectrometry suggest that target proteins include several carbohydrate-recognising proteins, which indicates that the sugar scaffold may have a role for target recognition.

Original language	English
Article number	115900
Number of pages	8
Journal	Bioorganic and Medicinal Chemistry
Volume	30
DOIs	https://doi.org/10.1016/j.bmc.2020.115900
Publication status	Published - 15 Jan 2021

Bibliographical note

Funding Information:
We thank King's College London for a studentship (to CCM) and a King's China Award (to YX), the EPSRC National Mass Spectrometry Facility, Swansea, for the recording of mass spectra, and Dr Andrew Atkinson (King's College London) for recording the NMR spectra of 5-?. This work was supported by an EPSRC Impact Acceleration Award (EP/R511559/1).

Funding Information:
We thank King’s College London for a studentship (to CCM) and a King’s China Award (to YX), the EPSRC National Mass Spectrometry Facility, Swansea, for the recording of mass spectra, and Dr Andrew Atkinson (King’s College London) for recording the NMR spectra of 5-β . This work was supported by an EPSRC Impact Acceleration Award (EP/R511559/1).

Keywords

Antimicrobial resistance
Bacterial profiling
Carbohydrate
Covalent probe
Klebsiella

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title = "Profiling protein expression in Klebsiella pneumoniae with a carbohydrate-based covalent probe",

abstract = "We report the application of a covalent probe based on a D-glucosamine scaffold for the profiling of the bacterial pathogen Klebsiella pneumoniae. Incubation of K. pneumoniae lysates with the probe followed by electrophoretic separation and in-gel fluorescence detection allowed the generation of strain-specific signatures and the differentiation of a carbapenem-resistant strain. The labelling profile of the probe was independent of its anomeric configuration and included several low-abundance proteins not readily detectable by conventional protein staining. Initial target identification experiments by mass spectrometry suggest that target proteins include several carbohydrate-recognising proteins, which indicates that the sugar scaffold may have a role for target recognition.",

keywords = "Antimicrobial resistance, Bacterial profiling, Carbohydrate, Covalent probe, Klebsiella",

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note = "Funding Information: We thank King's College London for a studentship (to CCM) and a King's China Award (to YX), the EPSRC National Mass Spectrometry Facility, Swansea, for the recording of mass spectra, and Dr Andrew Atkinson (King's College London) for recording the NMR spectra of 5-?. This work was supported by an EPSRC Impact Acceleration Award (EP/R511559/1). Funding Information: We thank King{\textquoteright}s College London for a studentship (to CCM) and a King{\textquoteright}s China Award (to YX), the EPSRC National Mass Spectrometry Facility, Swansea, for the recording of mass spectra, and Dr Andrew Atkinson (King{\textquoteright}s College London) for recording the NMR spectra of 5-β . This work was supported by an EPSRC Impact Acceleration Award (EP/R511559/1). ",

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AU - Lynham, Steven

AU - Zadi, Sirine

AU - Turner, Carrie

AU - Wand, Matthew

AU - Mark Sutton, J.

AU - Wagner, Gerd K.

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AB - We report the application of a covalent probe based on a D-glucosamine scaffold for the profiling of the bacterial pathogen Klebsiella pneumoniae. Incubation of K. pneumoniae lysates with the probe followed by electrophoretic separation and in-gel fluorescence detection allowed the generation of strain-specific signatures and the differentiation of a carbapenem-resistant strain. The labelling profile of the probe was independent of its anomeric configuration and included several low-abundance proteins not readily detectable by conventional protein staining. Initial target identification experiments by mass spectrometry suggest that target proteins include several carbohydrate-recognising proteins, which indicates that the sugar scaffold may have a role for target recognition.

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Profiling protein expression in Klebsiella pneumoniae with a carbohydrate-based covalent probe

Abstract

Bibliographical note

Keywords

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