Profiling protein expression in Klebsiella pneumoniae with a carbohydrate-based covalent probe

Camille C. Metier, Jiaming Peng, Yong Xu, Hayley Wootton, Victoire Riesi, Steven Lynham, Sirine Zadi, Carrie Turner, Matthew Wand, J. Mark Sutton, Gerd K. Wagner*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

We report the application of a covalent probe based on a D-glucosamine scaffold for the profiling of the bacterial pathogen Klebsiella pneumoniae. Incubation of K. pneumoniae lysates with the probe followed by electrophoretic separation and in-gel fluorescence detection allowed the generation of strain-specific signatures and the differentiation of a carbapenem-resistant strain. The labelling profile of the probe was independent of its anomeric configuration and included several low-abundance proteins not readily detectable by conventional protein staining. Initial target identification experiments by mass spectrometry suggest that target proteins include several carbohydrate-recognising proteins, which indicates that the sugar scaffold may have a role for target recognition.

Original languageEnglish
Article number115900
Number of pages8
JournalBioorganic and Medicinal Chemistry
Volume30
DOIs
Publication statusPublished - 15 Jan 2021

Bibliographical note

Funding Information:
We thank King's College London for a studentship (to CCM) and a King's China Award (to YX), the EPSRC National Mass Spectrometry Facility, Swansea, for the recording of mass spectra, and Dr Andrew Atkinson (King's College London) for recording the NMR spectra of 5-?. This work was supported by an EPSRC Impact Acceleration Award (EP/R511559/1).

Funding Information:
We thank King’s College London for a studentship (to CCM) and a King’s China Award (to YX), the EPSRC National Mass Spectrometry Facility, Swansea, for the recording of mass spectra, and Dr Andrew Atkinson (King’s College London) for recording the NMR spectra of 5-β . This work was supported by an EPSRC Impact Acceleration Award (EP/R511559/1).

Keywords

  • Antimicrobial resistance
  • Bacterial profiling
  • Carbohydrate
  • Covalent probe
  • Klebsiella

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