TY - JOUR
T1 - Polymerase chain reaction in the detection of an 'outbreak' of asymptomatic viral infections in a community birth cohort in south India
AU - Gladstone, B. P.
AU - Iturriza-Gomara, M.
AU - Ramani, S.
AU - Monica, B.
AU - Banerjee, I.
AU - Brown, David
AU - Gray, J. J.
AU - Muliyil, J.
AU - Kang, G.
PY - 2008/3
Y1 - 2008/3
N2 - Asymptomatic enteric infections are important where sequelae or protection from subsequent illness is an outcome measure. The use of reverse transcription-polymerase chain reaction (RT-PCR) to identify asymptomatic enteric infections in a birth cohort followed for rotaviral infections in a south Indian urban slum is reported. Of 1191 non-diarrhoeal samples from 371 children collected in May-June 2003, 22 (1.9%) were positive by ELISA. A total of 147 (40.6%) of 362 samples tested by VP6 RT-PCR were positive. In those samples that could be typed, a high diversity of G types including G1, G2, G4, G8, G9 and G10, and a high proportion (34.4%) of mixed infections were detected. Noroviruses were identified in 6/28 (21.4%) samples tested. The identification of infections undetectable by conventional techniques indicates the importance of the use of sensitive diagnostic techniques in research studies. Asymptomatically infected children may also act as a source of infection for other susceptible hosts.
AB - Asymptomatic enteric infections are important where sequelae or protection from subsequent illness is an outcome measure. The use of reverse transcription-polymerase chain reaction (RT-PCR) to identify asymptomatic enteric infections in a birth cohort followed for rotaviral infections in a south Indian urban slum is reported. Of 1191 non-diarrhoeal samples from 371 children collected in May-June 2003, 22 (1.9%) were positive by ELISA. A total of 147 (40.6%) of 362 samples tested by VP6 RT-PCR were positive. In those samples that could be typed, a high diversity of G types including G1, G2, G4, G8, G9 and G10, and a high proportion (34.4%) of mixed infections were detected. Noroviruses were identified in 6/28 (21.4%) samples tested. The identification of infections undetectable by conventional techniques indicates the importance of the use of sensitive diagnostic techniques in research studies. Asymptomatically infected children may also act as a source of infection for other susceptible hosts.
UR - http://www.scopus.com/inward/record.url?scp=39049146926&partnerID=8YFLogxK
U2 - 10.1017/S0950268807008709
DO - 10.1017/S0950268807008709
M3 - Article
C2 - 17521476
AN - SCOPUS:39049146926
SN - 0950-2688
VL - 136
SP - 399
EP - 405
JO - Epidemiology and Infection
JF - Epidemiology and Infection
IS - 3
ER -
