PiuA and PiaA, iron uptake lipoproteins of Streptococcus pneumoniae, elicit serotype independent antibody responses following human pneumococcal septicaemia

Rachael H. Whalan; Simon G.P. Funnell; Lucas D. Bowler; Michael J. Hudson; Andrew Robinson; Christopher G. Dowson

doi:10.1016/j.femsim.2004.07.010

PiuA and PiaA, iron uptake lipoproteins of Streptococcus pneumoniae, elicit serotype independent antibody responses following human pneumococcal septicaemia

Rachael H. Whalan, Simon G.P. Funnell, Lucas D. Bowler, Michael J. Hudson, Andrew Robinson, Christopher G. Dowson

Research & Development Institute Scientific Leaders

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23 Citations (Scopus)

Abstract

Streptococcus pneumoniae causes considerable morbidity and mortality worldwide. The need for a cheap and effective pneumococcal vaccine has necessitated the evaluation of common virulence-associated proteins as potential vaccine antigens. PiuA and PiaA are the lipoprotein components of two pneumococcal iron ABC transporters. Here, we show that patients with culture confirmed pneumococcal septicaemia have elevated levels of antibody to PiuA and PiaA in convalescent-phase, compared with acute-phase serum. Additionally, sera from septicaemic patients infected with 13 pneumococcal strains covering eight different serotypes, cross-reacted with recombinant PiuA-His ₆ and PiaA-His ₆ from a single pneumococcal strain, indicating that this immune response is serotype independent. Anti-PiuA and anti-PiaA antibodies were also found in healthy seven-month-old infants, indicating that they are immunogenic at a very early age.

Original language	English
Pages (from-to)	73-80
Number of pages	8
Journal	FEMS Immunology and Medical Microbiology
Volume	43
Issue number	1
DOIs	https://doi.org/10.1016/j.femsim.2004.07.010
Publication status	Published - 1 Jan 2005

Bibliographical note

Funding Information:
This work was supported through funding from the UK Department of Health by the Health Protection Agency, Porton Down (formerly Centre For Applied Microbiology And Research). The views expressed in this publication are those of the authors and not necessarily those of the Department of Health. We thank Dr. A. Lloyd and Dr. D. Roper (University of Warwick) for expert technical assistance, L. Foster, R. Winn and P. Hawtin (Public Health Laboratory, Southampton General Hospital) for collection and supply of human acute and convalescent phase sera, Dr. Maija Korkeila (KTL, Helsinki, Finland) for the supply of infant sera, and Professor Tim Mitchell (University of Glasgow) for the gift of purified pneumolysin.

Keywords

Antigens
Human
Iron
Streptococcus pneumoniae

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.femsim.2004.07.010

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title = "PiuA and PiaA, iron uptake lipoproteins of Streptococcus pneumoniae, elicit serotype independent antibody responses following human pneumococcal septicaemia",

abstract = "Streptococcus pneumoniae causes considerable morbidity and mortality worldwide. The need for a cheap and effective pneumococcal vaccine has necessitated the evaluation of common virulence-associated proteins as potential vaccine antigens. PiuA and PiaA are the lipoprotein components of two pneumococcal iron ABC transporters. Here, we show that patients with culture confirmed pneumococcal septicaemia have elevated levels of antibody to PiuA and PiaA in convalescent-phase, compared with acute-phase serum. Additionally, sera from septicaemic patients infected with 13 pneumococcal strains covering eight different serotypes, cross-reacted with recombinant PiuA-His 6 and PiaA-His 6 from a single pneumococcal strain, indicating that this immune response is serotype independent. Anti-PiuA and anti-PiaA antibodies were also found in healthy seven-month-old infants, indicating that they are immunogenic at a very early age.",

keywords = "Antigens, Human, Iron, Streptococcus pneumoniae",

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note = "Funding Information: This work was supported through funding from the UK Department of Health by the Health Protection Agency, Porton Down (formerly Centre For Applied Microbiology And Research). The views expressed in this publication are those of the authors and not necessarily those of the Department of Health. We thank Dr. A. Lloyd and Dr. D. Roper (University of Warwick) for expert technical assistance, L. Foster, R. Winn and P. Hawtin (Public Health Laboratory, Southampton General Hospital) for collection and supply of human acute and convalescent phase sera, Dr. Maija Korkeila (KTL, Helsinki, Finland) for the supply of infant sera, and Professor Tim Mitchell (University of Glasgow) for the gift of purified pneumolysin. ",

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PiuA and PiaA, iron uptake lipoproteins of Streptococcus pneumoniae, elicit serotype independent antibody responses following human pneumococcal septicaemia. / Whalan, Rachael H.; Funnell, Simon G.P.; Bowler, Lucas D. et al.
In: FEMS Immunology and Medical Microbiology, Vol. 43, No. 1, 01.01.2005, p. 73-80.

Research output: Contribution to journal › Article › peer-review

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AU - Hudson, Michael J.

AU - Robinson, Andrew

AU - Dowson, Christopher G.

N1 - Funding Information: This work was supported through funding from the UK Department of Health by the Health Protection Agency, Porton Down (formerly Centre For Applied Microbiology And Research). The views expressed in this publication are those of the authors and not necessarily those of the Department of Health. We thank Dr. A. Lloyd and Dr. D. Roper (University of Warwick) for expert technical assistance, L. Foster, R. Winn and P. Hawtin (Public Health Laboratory, Southampton General Hospital) for collection and supply of human acute and convalescent phase sera, Dr. Maija Korkeila (KTL, Helsinki, Finland) for the supply of infant sera, and Professor Tim Mitchell (University of Glasgow) for the gift of purified pneumolysin.

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AB - Streptococcus pneumoniae causes considerable morbidity and mortality worldwide. The need for a cheap and effective pneumococcal vaccine has necessitated the evaluation of common virulence-associated proteins as potential vaccine antigens. PiuA and PiaA are the lipoprotein components of two pneumococcal iron ABC transporters. Here, we show that patients with culture confirmed pneumococcal septicaemia have elevated levels of antibody to PiuA and PiaA in convalescent-phase, compared with acute-phase serum. Additionally, sera from septicaemic patients infected with 13 pneumococcal strains covering eight different serotypes, cross-reacted with recombinant PiuA-His 6 and PiaA-His 6 from a single pneumococcal strain, indicating that this immune response is serotype independent. Anti-PiuA and anti-PiaA antibodies were also found in healthy seven-month-old infants, indicating that they are immunogenic at a very early age.

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PiuA and PiaA, iron uptake lipoproteins of Streptococcus pneumoniae, elicit serotype independent antibody responses following human pneumococcal septicaemia

Abstract

Bibliographical note

Keywords

UN SDGs

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