Parvovirus PARV4 visualization and detection

Philip W. Tuke, Ruth P. Parry, Hazel Appleton*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    18 Citations (Scopus)


    The parvovirus PARV4 is the most recently described member of the family Parvoviridae that has a human host. To investigate the prevalence of PARV4 in blood, a quantitative TaqMan PCR was developed and plasma, sera or whole blood from a variety of population groups were examined. Eight samples were positive for PARV4, one at high copy number. The high-titre-positive plasma had an approximate viral load of 5×108 genome equivalents ml-1. Two human sera, identified as PARV4 antibody-positive by indirect immunofluorescence, were used in immune electron microscopy to try to visualize native PARV4 within the high-titre human plasma. PARV4 particles were observed using one of these two sera. To our knowledge, this is the first time that native PARV4 has been visualized.

    Original languageEnglish
    Pages (from-to)541-544
    Number of pages4
    JournalJournal of General Virology
    Issue number2
    Publication statusPublished - 2010


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