TY - JOUR
T1 - Parvovirus PARV4 visualization and detection
AU - Tuke, Philip W.
AU - Parry, Ruth P.
AU - Appleton, Hazel
PY - 2010
Y1 - 2010
N2 - The parvovirus PARV4 is the most recently described member of the family Parvoviridae that has a human host. To investigate the prevalence of PARV4 in blood, a quantitative TaqMan PCR was developed and plasma, sera or whole blood from a variety of population groups were examined. Eight samples were positive for PARV4, one at high copy number. The high-titre-positive plasma had an approximate viral load of 5×108 genome equivalents ml-1. Two human sera, identified as PARV4 antibody-positive by indirect immunofluorescence, were used in immune electron microscopy to try to visualize native PARV4 within the high-titre human plasma. PARV4 particles were observed using one of these two sera. To our knowledge, this is the first time that native PARV4 has been visualized.
AB - The parvovirus PARV4 is the most recently described member of the family Parvoviridae that has a human host. To investigate the prevalence of PARV4 in blood, a quantitative TaqMan PCR was developed and plasma, sera or whole blood from a variety of population groups were examined. Eight samples were positive for PARV4, one at high copy number. The high-titre-positive plasma had an approximate viral load of 5×108 genome equivalents ml-1. Two human sera, identified as PARV4 antibody-positive by indirect immunofluorescence, were used in immune electron microscopy to try to visualize native PARV4 within the high-titre human plasma. PARV4 particles were observed using one of these two sera. To our knowledge, this is the first time that native PARV4 has been visualized.
UR - http://www.scopus.com/inward/record.url?scp=76249131617&partnerID=8YFLogxK
U2 - 10.1099/vir.0.014852-0
DO - 10.1099/vir.0.014852-0
M3 - Article
C2 - 19846677
AN - SCOPUS:76249131617
SN - 0022-1317
VL - 91
SP - 541
EP - 544
JO - Journal of General Virology
JF - Journal of General Virology
IS - 2
ER -