The debate over the suitability of molecular biological methods for the enumeration of regulatory microbial parameters (e.g. Faecal Indicator Organisms [FIOs]) in bathing waters versus the use of traditional culture-based methods is of current interest to regulators and the science community. Culture-based methods require a 24-48. hour turn-around time from receipt at the laboratory to reporting, whilst quantitative molecular tools provide a more rapid assay (approximately 2-3. h). Traditional culturing methods are therefore often viewed as slow and 'out-dated', although they still deliver an internationally 'accepted' evidence-base. In contrast, molecular tools have the potential for rapid analysis and their operational utility and associated limitations and uncertainties should be assessed in light of their use for regulatory monitoring. Here we report on the recommendations from a series of international workshops, chaired by a UK Working Group (WG) comprised of scientists, regulators, policy makers and other stakeholders, which explored and interrogated both molecular (principally quantitative polymerase chain reaction [qPCR]) and culture-based tools for FIO monitoring under the European Bathing Water Directive. Through detailed analysis of policy implications, regulatory barriers, stakeholder engagement, and the needs of the end-user, the WG identified a series of key concerns that require critical appraisal before a potential shift from culture-based approaches to the employment of molecular biological methods for bathing water regulation could be justified.
|Number of pages||5|
|Publication status||Published - Mar 2014|
Bibliographical noteFunding Information:
The Working Group and associated workshop series were funded by the Natural Environment Research Council as part of the Delivering Healthy Water project ( NE/I022191/1 ). LF received funding in part from the European Regional Development Fund Programme and the European Social Fund Convergence Programme for Cornwall and the Isles of Scilly . The authors acknowledge the valuable contributions made by Tim Wade at the workshops in London and the constructive comments of the anonymous referees.
- EU Bathing Water Directive
- Faecal indicator organism
- Microbial pollution
- Recreational water