Neutralisation of biological activities of pertussis toxin with a monoclonal antibody

H. Anwar; L. A.E. Ashworth; S. Funnell; A. Robinson; L. I. Irons

doi:10.1111/j.1574-6968.1987.tb02258.x

Neutralisation of biological activities of pertussis toxin with a monoclonal antibody

H. Anwar^*, L. A.E. Ashworth, S. Funnell, A. Robinson, L. I. Irons

^*Corresponding author for this work

Research & Development Institute Scientific Leaders

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Three monoclonal antibodies (L5, L7 and L10) against pertussis toxin (PT) were produced by the fusion of myeloma cells with spleen cells of mice immunised with pertussis toxoid and PT. Immunoblotting showed that each monoclonal antibody bound to more than one subunit of the toxin. L5 and L7 bound predominantly to subunit S3 and with much less affinity to subunit S1, while L10 bound mainly to subunit S1. L7 strongly neutralised the lymphocytosis-promoting and histamine-sensitising activities of PT, whereas L5 and L10 showed no or only partial neutralising effect on these activities. The strongest neutralisation of PT activity in haemagglutination and Chinese hamster ovary (CHO) cell assays was also found with L7.

Original language	English
Pages (from-to)	141-145
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	44
Issue number	1
DOIs	https://doi.org/10.1111/j.1574-6968.1987.tb02258.x
Publication status	Published - Sept 1987

Keywords

Bordetella pertussis
Immunoblotting
Monoclonal antibody
Pertussis toxin

Access to Document

10.1111/j.1574-6968.1987.tb02258.x

Cite this

@article{cf5780caeb0e44a681f3d90a5877ef68,

title = "Neutralisation of biological activities of pertussis toxin with a monoclonal antibody",

abstract = "Three monoclonal antibodies (L5, L7 and L10) against pertussis toxin (PT) were produced by the fusion of myeloma cells with spleen cells of mice immunised with pertussis toxoid and PT. Immunoblotting showed that each monoclonal antibody bound to more than one subunit of the toxin. L5 and L7 bound predominantly to subunit S3 and with much less affinity to subunit S1, while L10 bound mainly to subunit S1. L7 strongly neutralised the lymphocytosis-promoting and histamine-sensitising activities of PT, whereas L5 and L10 showed no or only partial neutralising effect on these activities. The strongest neutralisation of PT activity in haemagglutination and Chinese hamster ovary (CHO) cell assays was also found with L7.",

keywords = "Bordetella pertussis, Immunoblotting, Monoclonal antibody, Pertussis toxin",

author = "H. Anwar and Ashworth, {L. A.E.} and S. Funnell and A. Robinson and Irons, {L. I.}",

year = "1987",

month = sep,

doi = "10.1111/j.1574-6968.1987.tb02258.x",

language = "English",

volume = "44",

pages = "141--145",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Oxford University Press",

number = "1",

}

TY - JOUR

T1 - Neutralisation of biological activities of pertussis toxin with a monoclonal antibody

AU - Anwar, H.

AU - Ashworth, L. A.E.

AU - Funnell, S.

AU - Robinson, A.

AU - Irons, L. I.

PY - 1987/9

Y1 - 1987/9

N2 - Three monoclonal antibodies (L5, L7 and L10) against pertussis toxin (PT) were produced by the fusion of myeloma cells with spleen cells of mice immunised with pertussis toxoid and PT. Immunoblotting showed that each monoclonal antibody bound to more than one subunit of the toxin. L5 and L7 bound predominantly to subunit S3 and with much less affinity to subunit S1, while L10 bound mainly to subunit S1. L7 strongly neutralised the lymphocytosis-promoting and histamine-sensitising activities of PT, whereas L5 and L10 showed no or only partial neutralising effect on these activities. The strongest neutralisation of PT activity in haemagglutination and Chinese hamster ovary (CHO) cell assays was also found with L7.

AB - Three monoclonal antibodies (L5, L7 and L10) against pertussis toxin (PT) were produced by the fusion of myeloma cells with spleen cells of mice immunised with pertussis toxoid and PT. Immunoblotting showed that each monoclonal antibody bound to more than one subunit of the toxin. L5 and L7 bound predominantly to subunit S3 and with much less affinity to subunit S1, while L10 bound mainly to subunit S1. L7 strongly neutralised the lymphocytosis-promoting and histamine-sensitising activities of PT, whereas L5 and L10 showed no or only partial neutralising effect on these activities. The strongest neutralisation of PT activity in haemagglutination and Chinese hamster ovary (CHO) cell assays was also found with L7.

KW - Bordetella pertussis

KW - Immunoblotting

KW - Monoclonal antibody

KW - Pertussis toxin

UR - http://www.scopus.com/inward/record.url?scp=38249034367&partnerID=8YFLogxK

U2 - 10.1111/j.1574-6968.1987.tb02258.x

DO - 10.1111/j.1574-6968.1987.tb02258.x

M3 - Article

AN - SCOPUS:38249034367

SN - 0378-1097

VL - 44

SP - 141

EP - 145

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 1

ER -

Neutralisation of biological activities of pertussis toxin with a monoclonal antibody

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this