Multivalent liposome-based vaccines containing different serosubtypes of PorA protein induce cross-protective bactericidal immune responses against Neisseria meningitidis

Holly E. Humphries; Jeannette N. Williams; Ramona Blackstone; Keith A. Jolley; Ho M. Yuen; Myron Christodoulides; John E. Heckels

doi:10.1016/j.vaccine.2005.07.056

Multivalent liposome-based vaccines containing different serosubtypes of PorA protein induce cross-protective bactericidal immune responses against Neisseria meningitidis

Holly E. Humphries
, Jeannette N. Williams
, Ramona Blackstone
, Keith A. Jolley
, Ho M. Yuen
, Myron Christodoulides
, John E. Heckels^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

Four serosubtypes (P1.7, 16, P1.7-2, 4, P1.19, 15 and P1.5-1, 10-4) of the PorA outer-membrane protein of Neisseria meningitidis were purified as recombinant proteins and incorporated into liposomes to investigate their immunogenicity. Each serosubtype induced high levels of bactericidal activity against the homologous strain. In addition, liposome preparations containing multiple serosubtypes induced high levels of bactericidal activity against each of the four strains. Significantly, antisera raised against monovalent and multivalent liposomes also showed cross-reactive bactericidal activity against heterologous strains. These data demonstrate that multivalent liposome vaccines, containing multiple PorA serosubtypes, have the potential to provide protection against a broad range of meningococcal strains.

Original language	English
Pages (from-to)	36-44
Number of pages	9
Journal	Vaccine
Volume	24
Issue number	1
DOIs	https://doi.org/10.1016/j.vaccine.2005.07.056
Publication status	Published - 9 Jan 2006
Externally published	Yes

Bibliographical note

Funding Information:
The work in this study was supported by The Meningitis Trust, The University of Southampton Strategic Development Fund and Hope (Wessex Medical Trust).

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Immunogenicity
Multivalent liposomes
Neisseria meningitides
Recombinant PorA

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10.1016/j.vaccine.2005.07.056

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title = "Multivalent liposome-based vaccines containing different serosubtypes of PorA protein induce cross-protective bactericidal immune responses against Neisseria meningitidis",

abstract = "Four serosubtypes (P1.7, 16, P1.7-2, 4, P1.19, 15 and P1.5-1, 10-4) of the PorA outer-membrane protein of Neisseria meningitidis were purified as recombinant proteins and incorporated into liposomes to investigate their immunogenicity. Each serosubtype induced high levels of bactericidal activity against the homologous strain. In addition, liposome preparations containing multiple serosubtypes induced high levels of bactericidal activity against each of the four strains. Significantly, antisera raised against monovalent and multivalent liposomes also showed cross-reactive bactericidal activity against heterologous strains. These data demonstrate that multivalent liposome vaccines, containing multiple PorA serosubtypes, have the potential to provide protection against a broad range of meningococcal strains.",

keywords = "Immunogenicity, Multivalent liposomes, Neisseria meningitides, Recombinant PorA",

author = "Humphries, \{Holly E.\} and Williams, \{Jeannette N.\} and Ramona Blackstone and Jolley, \{Keith A.\} and Yuen, \{Ho M.\} and Myron Christodoulides and Heckels, \{John E.\}",

note = "Funding Information: The work in this study was supported by The Meningitis Trust, The University of Southampton Strategic Development Fund and Hope (Wessex Medical Trust).",

year = "2006",

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T1 - Multivalent liposome-based vaccines containing different serosubtypes of PorA protein induce cross-protective bactericidal immune responses against Neisseria meningitidis

AU - Humphries, Holly E.

AU - Williams, Jeannette N.

AU - Blackstone, Ramona

AU - Jolley, Keith A.

AU - Yuen, Ho M.

AU - Christodoulides, Myron

AU - Heckels, John E.

N1 - Funding Information: The work in this study was supported by The Meningitis Trust, The University of Southampton Strategic Development Fund and Hope (Wessex Medical Trust).

PY - 2006/1/9

Y1 - 2006/1/9

N2 - Four serosubtypes (P1.7, 16, P1.7-2, 4, P1.19, 15 and P1.5-1, 10-4) of the PorA outer-membrane protein of Neisseria meningitidis were purified as recombinant proteins and incorporated into liposomes to investigate their immunogenicity. Each serosubtype induced high levels of bactericidal activity against the homologous strain. In addition, liposome preparations containing multiple serosubtypes induced high levels of bactericidal activity against each of the four strains. Significantly, antisera raised against monovalent and multivalent liposomes also showed cross-reactive bactericidal activity against heterologous strains. These data demonstrate that multivalent liposome vaccines, containing multiple PorA serosubtypes, have the potential to provide protection against a broad range of meningococcal strains.

AB - Four serosubtypes (P1.7, 16, P1.7-2, 4, P1.19, 15 and P1.5-1, 10-4) of the PorA outer-membrane protein of Neisseria meningitidis were purified as recombinant proteins and incorporated into liposomes to investigate their immunogenicity. Each serosubtype induced high levels of bactericidal activity against the homologous strain. In addition, liposome preparations containing multiple serosubtypes induced high levels of bactericidal activity against each of the four strains. Significantly, antisera raised against monovalent and multivalent liposomes also showed cross-reactive bactericidal activity against heterologous strains. These data demonstrate that multivalent liposome vaccines, containing multiple PorA serosubtypes, have the potential to provide protection against a broad range of meningococcal strains.

KW - Immunogenicity

KW - Multivalent liposomes

KW - Neisseria meningitides

KW - Recombinant PorA

UR - https://www.scopus.com/pages/publications/28344436617

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DO - 10.1016/j.vaccine.2005.07.056

M3 - Article

C2 - 16105711

AN - SCOPUS:28344436617

SN - 0264-410X

VL - 24

SP - 36

EP - 44

JO - Vaccine

JF - Vaccine

IS - 1

ER -

Multivalent liposome-based vaccines containing different serosubtypes of PorA protein induce cross-protective bactericidal immune responses against Neisseria meningitidis

Abstract

Bibliographical note

UN SDGs

Keywords

Access to Document

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