Multitarget PCR assay for direct detection of penicillinase-producing neisseria gonorrhoeae for enhanced surveillance of gonococcal antimicrobial resistance

Cameron Buckley; Ella Trembizki; Robert W. Baird; Marcus Chen; Basil Donovan; Kevin Freeman; Namraj Goire; Rebecca Guy; Monica M. Lahra; David Regan; David M. Whiley

doi:10.1128/JCM.00540-15

Multitarget PCR assay for direct detection of penicillinase-producing neisseria gonorrhoeae for enhanced surveillance of gonococcal antimicrobial resistance

Cameron Buckley, Ella Trembizki, Robert W. Baird, Marcus Chen, Basil Donovan, Kevin Freeman, Namraj Goire, Rebecca Guy, Monica M. Lahra, David Regan, David M. Whiley^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

A multitarget PCR was developed for the direct detection of penicillinase-producing Neisseria gonorrhoeae (PPNG). The assay was validated by testing 342 PPNG isolates and 415 clinical samples. The method is suitable for routine detection of PPNG strains. Its multitarget approach reduces the potential for false-negative results caused by sequence variations.

Original language	English
Pages (from-to)	2706-2708
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	53
Issue number	8
DOIs	https://doi.org/10.1128/JCM.00540-15
Publication status	Published - 1 Aug 2015
Externally published	Yes

Bibliographical note

Publisher Copyright:
Copyright © 2015, American Society for Microbiology. All Rights Reserved.

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Buckley, C., Trembizki, E., Baird, R. W., Chen, M., Donovan, B., Freeman, K., Goire, N., Guy, R., Lahra, M. M., Regan, D., & Whiley, D. M. (2015). Multitarget PCR assay for direct detection of penicillinase-producing neisseria gonorrhoeae for enhanced surveillance of gonococcal antimicrobial resistance. Journal of Clinical Microbiology, 53(8), 2706-2708. https://doi.org/10.1128/JCM.00540-15

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Buckley, C, Trembizki, E, Baird, RW, Chen, M, Donovan, B, Freeman, K, Goire, N, Guy, R, Lahra, MM, Regan, D & Whiley, DM 2015, 'Multitarget PCR assay for direct detection of penicillinase-producing neisseria gonorrhoeae for enhanced surveillance of gonococcal antimicrobial resistance', Journal of Clinical Microbiology, vol. 53, no. 8, pp. 2706-2708. https://doi.org/10.1128/JCM.00540-15

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AU - Trembizki, Ella

AU - Baird, Robert W.

AU - Chen, Marcus

AU - Donovan, Basil

AU - Freeman, Kevin

AU - Goire, Namraj

AU - Guy, Rebecca

AU - Lahra, Monica M.

AU - Regan, David

AU - Whiley, David M.

PY - 2015/8/1

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Multitarget PCR assay for direct detection of penicillinase-producing neisseria gonorrhoeae for enhanced surveillance of gonococcal antimicrobial resistance

Abstract

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