Multiplex PCR for identification of two capsular types in epidemic KPC-producing Klebsiella pneumoniae sequence type 258 strains

Liang Chen; Kalyan D. Chavda; Jacqueline Findlay; Gisele Peirano; Katie Hopkins; Johann D.D. Pitout; Robert A. Bonomo; Neil Woodford; Frank R. DeLeo; Barry N. Kreiswirth

doi:10.1128/AAC.02673-14

Multiplex PCR for identification of two capsular types in epidemic KPC-producing Klebsiella pneumoniae sequence type 258 strains

Liang Chen, Kalyan D. Chavda, Jacqueline Findlay, Gisele Peirano, Katie Hopkins, Johann D.D. Pitout, Robert A. Bonomo, Neil Woodford, Frank R. DeLeo, Barry N. Kreiswirth^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

We developed a multiplex PCR assay capable of identifying two capsular polysaccharide synthesis sequence types (sequence type 258 [ST258] cps-1 and cps-2) in epidemic Klebsiella pneumoniae ST258 strains. The assay performed with excellent sensitivity (100%) and specificity (100%) for identifying cps types in 60 ST258 K. pneumoniae sequenced isolates. The screening of 419 ST258 clonal isolates revealed a significant association between cps type and K. pneumoniae carbapenemase (KPC) variant: cps-1 is largely associated with KPC-2, while cps-2 is primarily associated with KPC-3.

Original language	English
Pages (from-to)	4196-4199
Number of pages	4
Journal	Antimicrobial Agents and Chemotherapy
Volume	58
Issue number	7
DOIs	https://doi.org/10.1128/AAC.02673-14
Publication status	Published - Jul 2014

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Chen, L., Chavda, K. D., Findlay, J., Peirano, G., Hopkins, K., Pitout, J. D. D., Bonomo, R. A., Woodford, N., DeLeo, F. R., & Kreiswirth, B. N. (2014). Multiplex PCR for identification of two capsular types in epidemic KPC-producing Klebsiella pneumoniae sequence type 258 strains. Antimicrobial Agents and Chemotherapy, 58(7), 4196-4199. https://doi.org/10.1128/AAC.02673-14

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title = "Multiplex PCR for identification of two capsular types in epidemic KPC-producing Klebsiella pneumoniae sequence type 258 strains",

abstract = "We developed a multiplex PCR assay capable of identifying two capsular polysaccharide synthesis sequence types (sequence type 258 [ST258] cps-1 and cps-2) in epidemic Klebsiella pneumoniae ST258 strains. The assay performed with excellent sensitivity (100%) and specificity (100%) for identifying cps types in 60 ST258 K. pneumoniae sequenced isolates. The screening of 419 ST258 clonal isolates revealed a significant association between cps type and K. pneumoniae carbapenemase (KPC) variant: cps-1 is largely associated with KPC-2, while cps-2 is primarily associated with KPC-3.",

author = "Liang Chen and Chavda, {Kalyan D.} and Jacqueline Findlay and Gisele Peirano and Katie Hopkins and Pitout, {Johann D.D.} and Bonomo, {Robert A.} and Neil Woodford and DeLeo, {Frank R.} and Kreiswirth, {Barry N.}",

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AU - Chen, Liang

AU - Chavda, Kalyan D.

AU - Findlay, Jacqueline

AU - Peirano, Gisele

AU - Hopkins, Katie

AU - Pitout, Johann D.D.

AU - Bonomo, Robert A.

AU - Woodford, Neil

AU - DeLeo, Frank R.

AU - Kreiswirth, Barry N.

PY - 2014/7

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AB - We developed a multiplex PCR assay capable of identifying two capsular polysaccharide synthesis sequence types (sequence type 258 [ST258] cps-1 and cps-2) in epidemic Klebsiella pneumoniae ST258 strains. The assay performed with excellent sensitivity (100%) and specificity (100%) for identifying cps types in 60 ST258 K. pneumoniae sequenced isolates. The screening of 419 ST258 clonal isolates revealed a significant association between cps type and K. pneumoniae carbapenemase (KPC) variant: cps-1 is largely associated with KPC-2, while cps-2 is primarily associated with KPC-3.

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Multiplex PCR for identification of two capsular types in epidemic KPC-producing Klebsiella pneumoniae sequence type 258 strains

Abstract

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