Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp.

Neil Woodford; Matthew Ellington; Juliana M. Coelho; Jane Turton; M. Elaina Ward; Susan Brown; Sebastian G.B. Amyes; David M. Livermore

doi:10.1016/j.ijantimicag.2006.01.004

Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp.

Neil Woodford^*, Matthew Ellington, Juliana M. Coelho, Jane Turton, M. Elaina Ward, Susan Brown, Sebastian G.B. Amyes, David M. Livermore

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

Carbapenem resistance in Acinetobacter baumannii is a growing public health concern and is most often mediated by OXA carbapenemases. We describe a novel multiplex polymerase chain reaction (PCR) assay able to detect and distinguish alleles encoding three subgroups of acquired OXA carbapenemases (OXA-23-like, OXA-24-like and OXA-58-like) that are scattered in Acinetobacter spp., and a fourth subgroup, OXA-51-like, which appears to be intrinsic to Acinetobacter baumannii. Isolates belonging to two prevalent UK A. baumannii 'OXA' clones (OXA-23 clones 1 and 2) had alleles encoding both an intrinsic OXA-51-like and an acquired OXA-23 enzyme, whereas isolates of the 'SE clone' had only an intrinsic bla_OXA-51-like allele. Genes encoding OXA-58 were detected (with bla_OXA-51-like) in a cluster of related isolates from a single hospital. This simple assay will assist in monitoring the mechanisms responsible for carbapenem resistance in Acinetobacter spp.

Original language	English
Pages (from-to)	351-353
Number of pages	3
Journal	International Journal of Antimicrobial Agents
Volume	27
Issue number	4
DOIs	https://doi.org/10.1016/j.ijantimicag.2006.01.004
Publication status	Published - Apr 2006

Bibliographical note

Funding Information:
We are grateful to AstraZeneca for supporting the work of J.M.C. on carbapenem-resistant Acinetobacter. The work of N.W., D.M.L. and M.E.W. on emerging β-lactamases is supported, in part, by the EU/FP6-funded COBRA project (6-PCRD LSHM-CT-2003-503-335). The work of S.B. and S.G.B.A. was supported by the Chief Scientist Office of the Scottish Executive Health Department (grant number CZB/4/42). This work was presented in December 2005 at the 45th ICAAC, Washington, USA; Poster D-1706.

Keywords

Acinetobacter
Antibiotic resistance
Carbapenemases
Molecular diagnostics
OXA enzymes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ijantimicag.2006.01.004

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title = "Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp.",

abstract = "Carbapenem resistance in Acinetobacter baumannii is a growing public health concern and is most often mediated by OXA carbapenemases. We describe a novel multiplex polymerase chain reaction (PCR) assay able to detect and distinguish alleles encoding three subgroups of acquired OXA carbapenemases (OXA-23-like, OXA-24-like and OXA-58-like) that are scattered in Acinetobacter spp., and a fourth subgroup, OXA-51-like, which appears to be intrinsic to Acinetobacter baumannii. Isolates belonging to two prevalent UK A. baumannii 'OXA' clones (OXA-23 clones 1 and 2) had alleles encoding both an intrinsic OXA-51-like and an acquired OXA-23 enzyme, whereas isolates of the 'SE clone' had only an intrinsic blaOXA-51-like allele. Genes encoding OXA-58 were detected (with blaOXA-51-like) in a cluster of related isolates from a single hospital. This simple assay will assist in monitoring the mechanisms responsible for carbapenem resistance in Acinetobacter spp.",

keywords = "Acinetobacter, Antibiotic resistance, Carbapenemases, Molecular diagnostics, OXA enzymes",

author = "Neil Woodford and Matthew Ellington and Coelho, {Juliana M.} and Jane Turton and Ward, {M. Elaina} and Susan Brown and Amyes, {Sebastian G.B.} and Livermore, {David M.}",

note = "Funding Information: We are grateful to AstraZeneca for supporting the work of J.M.C. on carbapenem-resistant Acinetobacter. The work of N.W., D.M.L. and M.E.W. on emerging β-lactamases is supported, in part, by the EU/FP6-funded COBRA project (6-PCRD LSHM-CT-2003-503-335). The work of S.B. and S.G.B.A. was supported by the Chief Scientist Office of the Scottish Executive Health Department (grant number CZB/4/42). This work was presented in December 2005 at the 45th ICAAC, Washington, USA; Poster D-1706.",

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AU - Turton, Jane

AU - Ward, M. Elaina

AU - Brown, Susan

AU - Amyes, Sebastian G.B.

AU - Livermore, David M.

N1 - Funding Information: We are grateful to AstraZeneca for supporting the work of J.M.C. on carbapenem-resistant Acinetobacter. The work of N.W., D.M.L. and M.E.W. on emerging β-lactamases is supported, in part, by the EU/FP6-funded COBRA project (6-PCRD LSHM-CT-2003-503-335). The work of S.B. and S.G.B.A. was supported by the Chief Scientist Office of the Scottish Executive Health Department (grant number CZB/4/42). This work was presented in December 2005 at the 45th ICAAC, Washington, USA; Poster D-1706.

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N2 - Carbapenem resistance in Acinetobacter baumannii is a growing public health concern and is most often mediated by OXA carbapenemases. We describe a novel multiplex polymerase chain reaction (PCR) assay able to detect and distinguish alleles encoding three subgroups of acquired OXA carbapenemases (OXA-23-like, OXA-24-like and OXA-58-like) that are scattered in Acinetobacter spp., and a fourth subgroup, OXA-51-like, which appears to be intrinsic to Acinetobacter baumannii. Isolates belonging to two prevalent UK A. baumannii 'OXA' clones (OXA-23 clones 1 and 2) had alleles encoding both an intrinsic OXA-51-like and an acquired OXA-23 enzyme, whereas isolates of the 'SE clone' had only an intrinsic blaOXA-51-like allele. Genes encoding OXA-58 were detected (with blaOXA-51-like) in a cluster of related isolates from a single hospital. This simple assay will assist in monitoring the mechanisms responsible for carbapenem resistance in Acinetobacter spp.

AB - Carbapenem resistance in Acinetobacter baumannii is a growing public health concern and is most often mediated by OXA carbapenemases. We describe a novel multiplex polymerase chain reaction (PCR) assay able to detect and distinguish alleles encoding three subgroups of acquired OXA carbapenemases (OXA-23-like, OXA-24-like and OXA-58-like) that are scattered in Acinetobacter spp., and a fourth subgroup, OXA-51-like, which appears to be intrinsic to Acinetobacter baumannii. Isolates belonging to two prevalent UK A. baumannii 'OXA' clones (OXA-23 clones 1 and 2) had alleles encoding both an intrinsic OXA-51-like and an acquired OXA-23 enzyme, whereas isolates of the 'SE clone' had only an intrinsic blaOXA-51-like allele. Genes encoding OXA-58 were detected (with blaOXA-51-like) in a cluster of related isolates from a single hospital. This simple assay will assist in monitoring the mechanisms responsible for carbapenem resistance in Acinetobacter spp.

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SN - 0924-8579

VL - 27

SP - 351

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JO - International Journal of Antimicrobial Agents

JF - International Journal of Antimicrobial Agents

IS - 4

ER -

Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp.

Abstract

Bibliographical note

Keywords

UN SDGs

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