TY - JOUR
T1 - Multiple norovirus genotypes characterised from an oyster-associated outbreak of gastroenteritis
AU - Gallimore, Christopher
AU - Cheesbrough, John S.
AU - Lamden, Kenneth
AU - Bingham, Chris
AU - Gray, Jim J.
PY - 2005/9/15
Y1 - 2005/9/15
N2 - The diversity of norovirus (NV) genotypes was investigated in persons who were ill with acute gastroenteritis associated with the consumption of oysters. Initial results from a commercial enzyme immunoassay (EIA) indicated a mixed NV genogroup I (GI) and II (GII) outbreak. A reverse-transcriptase (RT)-PCR for NVs was applied to nucleic acid extracted from faecal specimens collected from symptomatic cases. Using primers that amplified contiguous sequences in the ORF1/2 region of the NV genome and a hemi-nested PCR derived from this assay, three different GII and two GI NV genotypes were detected and the strains were characterised by DNA sequencing. Using this approach a recombinant NV genotype, rGII-3a (recombinant Harrow/Mexico) the predominant strain identified in several symptomatic cases from the outbreak, was detected and characterised. No other gastroenteric viruses, including rotavirus, astrovirus, sapovirus and adenovirus 40/41 were detected by RT-PCR and PCR.
AB - The diversity of norovirus (NV) genotypes was investigated in persons who were ill with acute gastroenteritis associated with the consumption of oysters. Initial results from a commercial enzyme immunoassay (EIA) indicated a mixed NV genogroup I (GI) and II (GII) outbreak. A reverse-transcriptase (RT)-PCR for NVs was applied to nucleic acid extracted from faecal specimens collected from symptomatic cases. Using primers that amplified contiguous sequences in the ORF1/2 region of the NV genome and a hemi-nested PCR derived from this assay, three different GII and two GI NV genotypes were detected and the strains were characterised by DNA sequencing. Using this approach a recombinant NV genotype, rGII-3a (recombinant Harrow/Mexico) the predominant strain identified in several symptomatic cases from the outbreak, was detected and characterised. No other gastroenteric viruses, including rotavirus, astrovirus, sapovirus and adenovirus 40/41 were detected by RT-PCR and PCR.
KW - Multiple strains
KW - Norovirus
KW - Oysters
KW - Recombinants
UR - http://www.scopus.com/inward/record.url?scp=23744441977&partnerID=8YFLogxK
U2 - 10.1016/j.ijfoodmicro.2005.02.003
DO - 10.1016/j.ijfoodmicro.2005.02.003
M3 - Article
C2 - 15967530
AN - SCOPUS:23744441977
SN - 0168-1605
VL - 103
SP - 323
EP - 330
JO - International Journal of Food Microbiology
JF - International Journal of Food Microbiology
IS - 3
ER -