Multiple norovirus genotypes characterised from an oyster-associated outbreak of gastroenteritis

Christopher Gallimore*, John S. Cheesbrough, Kenneth Lamden, Chris Bingham, Jim J. Gray

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

88 Citations (Scopus)

Abstract

The diversity of norovirus (NV) genotypes was investigated in persons who were ill with acute gastroenteritis associated with the consumption of oysters. Initial results from a commercial enzyme immunoassay (EIA) indicated a mixed NV genogroup I (GI) and II (GII) outbreak. A reverse-transcriptase (RT)-PCR for NVs was applied to nucleic acid extracted from faecal specimens collected from symptomatic cases. Using primers that amplified contiguous sequences in the ORF1/2 region of the NV genome and a hemi-nested PCR derived from this assay, three different GII and two GI NV genotypes were detected and the strains were characterised by DNA sequencing. Using this approach a recombinant NV genotype, rGII-3a (recombinant Harrow/Mexico) the predominant strain identified in several symptomatic cases from the outbreak, was detected and characterised. No other gastroenteric viruses, including rotavirus, astrovirus, sapovirus and adenovirus 40/41 were detected by RT-PCR and PCR.

Original languageEnglish
Pages (from-to)323-330
Number of pages8
JournalInternational Journal of Food Microbiology
Volume103
Issue number3
DOIs
Publication statusPublished - 15 Sep 2005

Keywords

  • Multiple strains
  • Norovirus
  • Oysters
  • Recombinants

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