Multilocus sequence typing directly on DNA from clinical samples and a cultured isolate to investigate linked fatal pneumococcal disease in residents of a shelter for homeless men

Andrew Birtles; Noel McCarthy; Carmen L. Sheppard; Harry Rutter; Malcolm Guiver; Elizabeth Haworth; Robert George

doi:10.1128/JCM.43.4.2004-2008.2005

Multilocus sequence typing directly on DNA from clinical samples and a cultured isolate to investigate linked fatal pneumococcal disease in residents of a shelter for homeless men

Andrew Birtles, Noel McCarthy, Carmen L. Sheppard, Harry Rutter, Malcolm Guiver, Elizabeth Haworth, Robert George^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Two apparently linked fatal cases of pneumococcal meningitis were investigated. Pneumolysin PCR performed on blood and cerebrospinal fluid was positive in a culture-negative case. A second case yielded Streptococcus pneumoniae from blood culture. Multilocus sequence typing performed on DNA extracted from case 1 (specimens) and case 2 (isolate) revealed identical sequence types (ST53) substantiating the link between them.

Original language	English
Pages (from-to)	2004-2008
Number of pages	5
Journal	Journal of Clinical Microbiology
Volume	43
Issue number	4
DOIs	https://doi.org/10.1128/JCM.43.4.2004-2008.2005
Publication status	Published - Apr 2005

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Birtles, A., McCarthy, N., Sheppard, C. L., Rutter, H., Guiver, M., Haworth, E., & George, R. (2005). Multilocus sequence typing directly on DNA from clinical samples and a cultured isolate to investigate linked fatal pneumococcal disease in residents of a shelter for homeless men. Journal of Clinical Microbiology, 43(4), 2004-2008. https://doi.org/10.1128/JCM.43.4.2004-2008.2005

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abstract = "Two apparently linked fatal cases of pneumococcal meningitis were investigated. Pneumolysin PCR performed on blood and cerebrospinal fluid was positive in a culture-negative case. A second case yielded Streptococcus pneumoniae from blood culture. Multilocus sequence typing performed on DNA extracted from case 1 (specimens) and case 2 (isolate) revealed identical sequence types (ST53) substantiating the link between them.",

author = "Andrew Birtles and Noel McCarthy and Sheppard, {Carmen L.} and Harry Rutter and Malcolm Guiver and Elizabeth Haworth and Robert George",

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Birtles, A, McCarthy, N, Sheppard, CL, Rutter, H, Guiver, M, Haworth, E & George, R 2005, 'Multilocus sequence typing directly on DNA from clinical samples and a cultured isolate to investigate linked fatal pneumococcal disease in residents of a shelter for homeless men', Journal of Clinical Microbiology, vol. 43, no. 4, pp. 2004-2008. https://doi.org/10.1128/JCM.43.4.2004-2008.2005

Multilocus sequence typing directly on DNA from clinical samples and a cultured isolate to investigate linked fatal pneumococcal disease in residents of a shelter for homeless men. / Birtles, Andrew; McCarthy, Noel; Sheppard, Carmen L. et al.
In: Journal of Clinical Microbiology, Vol. 43, No. 4, 04.2005, p. 2004-2008.

Research output: Contribution to journal › Article › peer-review

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AU - McCarthy, Noel

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AU - Guiver, Malcolm

AU - Haworth, Elizabeth

AU - George, Robert

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AB - Two apparently linked fatal cases of pneumococcal meningitis were investigated. Pneumolysin PCR performed on blood and cerebrospinal fluid was positive in a culture-negative case. A second case yielded Streptococcus pneumoniae from blood culture. Multilocus sequence typing performed on DNA extracted from case 1 (specimens) and case 2 (isolate) revealed identical sequence types (ST53) substantiating the link between them.

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Multilocus sequence typing directly on DNA from clinical samples and a cultured isolate to investigate linked fatal pneumococcal disease in residents of a shelter for homeless men

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