Multicentre evaluation of the BYG Carba v2.0 test, a simplified electrochemical assay for the rapid laboratory detection of carbapenemase-producing Enterobacteriaceae

Pierre Bogaerts; Saoussen Oueslati; Daniele Meunier; Claire Nonhoff; Sami Yunus; Marion Massart; Olivier Denis; Neil Woodford; Katie L. Hopkins; Thierry Naas; Laurent Dortet; Te Din Huang; Youri Glupczynski

doi:10.1038/s41598-017-09820-y

Multicentre evaluation of the BYG Carba v2.0 test, a simplified electrochemical assay for the rapid laboratory detection of carbapenemase-producing Enterobacteriaceae

Pierre Bogaerts^*
, Saoussen Oueslati
, Daniele Meunier
, Claire Nonhoff
, Sami Yunus
, Marion Massart
, Olivier Denis
, Neil Woodford
, Katie L. Hopkins
, Thierry Naas
, Laurent Dortet
, Te Din Huang
, Youri Glupczynski

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Rapid detection of carbapenemase-producing Enterobacteriaceae (CPE) represents a major challenge for microbiology laboratories. We evaluated the BYG Carba v2.0 using a simplified protocol, which detects CPE in less than 30 minutes. This new procedure reduces the hands-on-time from 5 to one minute and only requires a limited amount of material (one to three colonies) thereby preventing the need for subculturing bacterial isolates to reach a larger amount of pure biomass. This multicentre study involved four European reference laboratories. For the 1181 isolates tested across four centres, BYG Carba v2.0 yielded overall sensitivity and specificity of 96.3% (CI95: 94.5-97.5) and 99.7% (CI95: 98.6-100) respectively. Considering only the 670 consecutive isolates tested prospectively, the BYG Carba v2.0 displayed overall positive and negative predictive values of 99.7% (CI95: 95.4-98.9) and 97.5% (CI95: 94.9-98.8). Regarding time to positivity, 85% of CPE detected were positive within ten minutes. The BYG Carba v2.0 is a new highly simplified, rapid and accurate electrochemical assay discriminating between CPE and non-CPE in less than 30 min. The real-time quantified signal allows objective and traceable interpretation of the results.

Original language	English
Article number	9937
Journal	Scientific Reports
Volume	7
Issue number	1
DOIs	https://doi.org/10.1038/s41598-017-09820-y
Publication status	Published - 1 Dec 2017

Bibliographical note

Funding Information:
This work was partially supported by the Belgian Walloon region (POC project VALBYG and First Spin off E-Sens Convention 1117301). The funders had no role in study design, data collection and interpretation, or in the decision to submit the work for publication.

Publisher Copyright:
© 2017 The Author(s).

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10.1038/s41598-017-09820-y

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Bogaerts, P., Oueslati, S., Meunier, D., Nonhoff, C., Yunus, S., Massart, M., Denis, O., Woodford, N., Hopkins, K. L., Naas, T., Dortet, L., Huang, T. D., & Glupczynski, Y. (2017). Multicentre evaluation of the BYG Carba v2.0 test, a simplified electrochemical assay for the rapid laboratory detection of carbapenemase-producing Enterobacteriaceae. Scientific Reports, 7(1), Article 9937. https://doi.org/10.1038/s41598-017-09820-y

@article{b751846347bf48cca63e09e93b9160c1,

title = "Multicentre evaluation of the BYG Carba v2.0 test, a simplified electrochemical assay for the rapid laboratory detection of carbapenemase-producing Enterobacteriaceae",

abstract = "Rapid detection of carbapenemase-producing Enterobacteriaceae (CPE) represents a major challenge for microbiology laboratories. We evaluated the BYG Carba v2.0 using a simplified protocol, which detects CPE in less than 30 minutes. This new procedure reduces the hands-on-time from 5 to one minute and only requires a limited amount of material (one to three colonies) thereby preventing the need for subculturing bacterial isolates to reach a larger amount of pure biomass. This multicentre study involved four European reference laboratories. For the 1181 isolates tested across four centres, BYG Carba v2.0 yielded overall sensitivity and specificity of 96.3\% (CI95: 94.5-97.5) and 99.7\% (CI95: 98.6-100) respectively. Considering only the 670 consecutive isolates tested prospectively, the BYG Carba v2.0 displayed overall positive and negative predictive values of 99.7\% (CI95: 95.4-98.9) and 97.5\% (CI95: 94.9-98.8). Regarding time to positivity, 85\% of CPE detected were positive within ten minutes. The BYG Carba v2.0 is a new highly simplified, rapid and accurate electrochemical assay discriminating between CPE and non-CPE in less than 30 min. The real-time quantified signal allows objective and traceable interpretation of the results.",

author = "Pierre Bogaerts and Saoussen Oueslati and Daniele Meunier and Claire Nonhoff and Sami Yunus and Marion Massart and Olivier Denis and Neil Woodford and Hopkins, \{Katie L.\} and Thierry Naas and Laurent Dortet and Huang, \{Te Din\} and Youri Glupczynski",

note = "Funding Information: This work was partially supported by the Belgian Walloon region (POC project VALBYG and First Spin off E-Sens Convention 1117301). The funders had no role in study design, data collection and interpretation, or in the decision to submit the work for publication. Publisher Copyright: {\textcopyright} 2017 The Author(s).",

year = "2017",

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doi = "10.1038/s41598-017-09820-y",

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Bogaerts, P, Oueslati, S, Meunier, D, Nonhoff, C, Yunus, S, Massart, M, Denis, O, Woodford, N , Hopkins, KL, Naas, T, Dortet, L, Huang, TD & Glupczynski, Y 2017, 'Multicentre evaluation of the BYG Carba v2.0 test, a simplified electrochemical assay for the rapid laboratory detection of carbapenemase-producing Enterobacteriaceae', Scientific Reports, vol. 7, no. 1, 9937. https://doi.org/10.1038/s41598-017-09820-y

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T1 - Multicentre evaluation of the BYG Carba v2.0 test, a simplified electrochemical assay for the rapid laboratory detection of carbapenemase-producing Enterobacteriaceae

AU - Bogaerts, Pierre

AU - Oueslati, Saoussen

AU - Meunier, Daniele

AU - Nonhoff, Claire

AU - Yunus, Sami

AU - Massart, Marion

AU - Denis, Olivier

AU - Woodford, Neil

AU - Hopkins, Katie L.

AU - Naas, Thierry

AU - Dortet, Laurent

AU - Huang, Te Din

AU - Glupczynski, Youri

N1 - Funding Information: This work was partially supported by the Belgian Walloon region (POC project VALBYG and First Spin off E-Sens Convention 1117301). The funders had no role in study design, data collection and interpretation, or in the decision to submit the work for publication. Publisher Copyright: © 2017 The Author(s).

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Rapid detection of carbapenemase-producing Enterobacteriaceae (CPE) represents a major challenge for microbiology laboratories. We evaluated the BYG Carba v2.0 using a simplified protocol, which detects CPE in less than 30 minutes. This new procedure reduces the hands-on-time from 5 to one minute and only requires a limited amount of material (one to three colonies) thereby preventing the need for subculturing bacterial isolates to reach a larger amount of pure biomass. This multicentre study involved four European reference laboratories. For the 1181 isolates tested across four centres, BYG Carba v2.0 yielded overall sensitivity and specificity of 96.3% (CI95: 94.5-97.5) and 99.7% (CI95: 98.6-100) respectively. Considering only the 670 consecutive isolates tested prospectively, the BYG Carba v2.0 displayed overall positive and negative predictive values of 99.7% (CI95: 95.4-98.9) and 97.5% (CI95: 94.9-98.8). Regarding time to positivity, 85% of CPE detected were positive within ten minutes. The BYG Carba v2.0 is a new highly simplified, rapid and accurate electrochemical assay discriminating between CPE and non-CPE in less than 30 min. The real-time quantified signal allows objective and traceable interpretation of the results.

AB - Rapid detection of carbapenemase-producing Enterobacteriaceae (CPE) represents a major challenge for microbiology laboratories. We evaluated the BYG Carba v2.0 using a simplified protocol, which detects CPE in less than 30 minutes. This new procedure reduces the hands-on-time from 5 to one minute and only requires a limited amount of material (one to three colonies) thereby preventing the need for subculturing bacterial isolates to reach a larger amount of pure biomass. This multicentre study involved four European reference laboratories. For the 1181 isolates tested across four centres, BYG Carba v2.0 yielded overall sensitivity and specificity of 96.3% (CI95: 94.5-97.5) and 99.7% (CI95: 98.6-100) respectively. Considering only the 670 consecutive isolates tested prospectively, the BYG Carba v2.0 displayed overall positive and negative predictive values of 99.7% (CI95: 95.4-98.9) and 97.5% (CI95: 94.9-98.8). Regarding time to positivity, 85% of CPE detected were positive within ten minutes. The BYG Carba v2.0 is a new highly simplified, rapid and accurate electrochemical assay discriminating between CPE and non-CPE in less than 30 min. The real-time quantified signal allows objective and traceable interpretation of the results.

UR - https://www.scopus.com/pages/publications/85028607561

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DO - 10.1038/s41598-017-09820-y

M3 - Article

C2 - 28855689

AN - SCOPUS:85028607561

SN - 2045-2322

VL - 7

JO - Scientific Reports

JF - Scientific Reports

IS - 1

M1 - 9937

ER -

Multicentre evaluation of the BYG Carba v2.0 test, a simplified electrochemical assay for the rapid laboratory detection of carbapenemase-producing Enterobacteriaceae

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