Abstract
microRNAs are non-coding RNAs that regulate gene expression. A significant proportion of microRNAs is perfectly conserved across the vertebrate clade, including miR-140, which is specifically expressed in cartilage. Although it has been computationally predicted that a large majority of microRNA targets are conserved, experimental evidence for this hypothesis remains scarce. In this work we use mRNA expression profiles obtained after manipulation of miR-140 activity levels in human and chicken primary chondrocytes to explore the extent of miR-140 target conservation. Our data suggest that miR-140 has a large number of targets conserved between human and chicken and we validate one of these, BMP2. However, we also found a significant number of non-conserved targets in the two species. In addition, we found that a commercially available scrambled siRNA, which is regularly used as a negative control, regulate the.
Original language | English |
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Pages (from-to) | 607-615 |
Number of pages | 9 |
Journal | RNA Biology |
Volume | 8 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2011 |
Externally published | Yes |
Keywords
- BMP2
- Chondrocyte
- Target conservation
- miR-140
- miRNA target
- microRNA