Monkeypox virus isolation from longitudinal samples from four patients to infer risk of onwards transmission: an interim analysis

H. Callaby; K. Emery; M. Killip; T. Rampling; K. S. Richards; C. F. Houlihan

doi:10.1016/j.jhin.2023.03.009

Monkeypox virus isolation from longitudinal samples from four patients to infer risk of onwards transmission: an interim analysis

H. Callaby^*, K. Emery, M. Killip, T. Rampling, K. S. Richards, C. F. Houlihan

^*Corresponding author for this work

Porton Emerging Infections & Zoonosis

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Monkeypox virus (mpxv) is a DNA virus in the Orthopoxvirus genus which causes Mpox (previously monkeypox). Symptoms include fever, lymphadenopathy and vesicular lesions. There is limited evidence for the duration of mpxv infectivity. This study used cell culture as a proxy for infectivity. Clinical samples from four patients with Mpox were inoculated into African green monkey kidney (Vero E6) cells and monitored for cytopathic effects (CPE). From one patient, infectious mpxv was recovered 25 days after illness onset. Infectious virus was not isolated from samples with an Orthopoxvirus polymerase chain reaction (PCR) Ct value over 31.0, nor from urine.

Original language	English
Pages (from-to)	139-144
Number of pages	6
Journal	Journal of Hospital Infection
Volume	135
DOIs	https://doi.org/10.1016/j.jhin.2023.03.009
Publication status	Published - May 2023

Bibliographical note

Publisher Copyright:
© 2023

Keywords

Infectivity
Monkeypox
Mpox
Transmission
Viral culture

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jhin.2023.03.009

Cite this

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title = "Monkeypox virus isolation from longitudinal samples from four patients to infer risk of onwards transmission: an interim analysis",

abstract = "Monkeypox virus (mpxv) is a DNA virus in the Orthopoxvirus genus which causes Mpox (previously monkeypox). Symptoms include fever, lymphadenopathy and vesicular lesions. There is limited evidence for the duration of mpxv infectivity. This study used cell culture as a proxy for infectivity. Clinical samples from four patients with Mpox were inoculated into African green monkey kidney (Vero E6) cells and monitored for cytopathic effects (CPE). From one patient, infectious mpxv was recovered 25 days after illness onset. Infectious virus was not isolated from samples with an Orthopoxvirus polymerase chain reaction (PCR) Ct value over 31.0, nor from urine.",

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doi = "10.1016/j.jhin.2023.03.009",

language = "English",

volume = "135",

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T1 - Monkeypox virus isolation from longitudinal samples from four patients to infer risk of onwards transmission

T2 - an interim analysis

AU - Callaby, H.

AU - Emery, K.

AU - Killip, M.

AU - Rampling, T.

AU - Richards, K. S.

AU - Houlihan, C. F.

PY - 2023/5

Y1 - 2023/5

N2 - Monkeypox virus (mpxv) is a DNA virus in the Orthopoxvirus genus which causes Mpox (previously monkeypox). Symptoms include fever, lymphadenopathy and vesicular lesions. There is limited evidence for the duration of mpxv infectivity. This study used cell culture as a proxy for infectivity. Clinical samples from four patients with Mpox were inoculated into African green monkey kidney (Vero E6) cells and monitored for cytopathic effects (CPE). From one patient, infectious mpxv was recovered 25 days after illness onset. Infectious virus was not isolated from samples with an Orthopoxvirus polymerase chain reaction (PCR) Ct value over 31.0, nor from urine.

AB - Monkeypox virus (mpxv) is a DNA virus in the Orthopoxvirus genus which causes Mpox (previously monkeypox). Symptoms include fever, lymphadenopathy and vesicular lesions. There is limited evidence for the duration of mpxv infectivity. This study used cell culture as a proxy for infectivity. Clinical samples from four patients with Mpox were inoculated into African green monkey kidney (Vero E6) cells and monitored for cytopathic effects (CPE). From one patient, infectious mpxv was recovered 25 days after illness onset. Infectious virus was not isolated from samples with an Orthopoxvirus polymerase chain reaction (PCR) Ct value over 31.0, nor from urine.

KW - Infectivity

KW - Monkeypox

KW - Mpox

KW - Transmission

KW - Viral culture

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DO - 10.1016/j.jhin.2023.03.009

M3 - Article

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JO - Journal of Hospital Infection

JF - Journal of Hospital Infection

ER -

Monkeypox virus isolation from longitudinal samples from four patients to infer risk of onwards transmission: an interim analysis

Abstract

Bibliographical note

Keywords

UN SDGs

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