TY - JOUR
T1 - Molecular typing of the Mycobacterium tuberculosis strains circulating in central Russia
T2 - efficiency of spoligotyping and VNTR-MIRU
AU - Nikolaevskii, V. V.
AU - Balabanova, I. M.
AU - Brown, T.
AU - Drobniewski, F. A.
AU - Fedorin, I. M.
N1 - Copyright:
Copyright 2015 Medline is the source for the citation and abstract of this record.
PY - 2005
Y1 - 2005
N2 - Current problems of molecular epidemiology of the Mycobacterium tuberculosis strains circulating in Samara Region, Russia are discussed. A total of 190 isolates of Mycobacterium tuberculosis were typed using two PCR-based molecular methods. The cultures were isolated from civil and prison patients with pulmonary tuberculosis recruited from different tuberculosis institutions across the Samara region. The usefulness of spoligotyping and 15-locii VNTR-MIRU was assessed for genotyping of Mycobacterium in population with high prevalence of Beijing strains (67.9%) using statistical analyses that included calculation of Hunter-Gaston index. The VNTR-MIRU method was demonstrated to be more efficient and was characterized by higher discrimination (index 0.747) compare to spoligotyping (index 0.572). VNTR-MIRU loci 10, 26, 31, 39, 40 and ETR-A were mostly polymorphic and therefore recommended for use in screening. It could be performed by manual electrophoresid, provided that automated sequencing is not available.
AB - Current problems of molecular epidemiology of the Mycobacterium tuberculosis strains circulating in Samara Region, Russia are discussed. A total of 190 isolates of Mycobacterium tuberculosis were typed using two PCR-based molecular methods. The cultures were isolated from civil and prison patients with pulmonary tuberculosis recruited from different tuberculosis institutions across the Samara region. The usefulness of spoligotyping and 15-locii VNTR-MIRU was assessed for genotyping of Mycobacterium in population with high prevalence of Beijing strains (67.9%) using statistical analyses that included calculation of Hunter-Gaston index. The VNTR-MIRU method was demonstrated to be more efficient and was characterized by higher discrimination (index 0.747) compare to spoligotyping (index 0.572). VNTR-MIRU loci 10, 26, 31, 39, 40 and ETR-A were mostly polymorphic and therefore recommended for use in screening. It could be performed by manual electrophoresid, provided that automated sequencing is not available.
UR - http://www.scopus.com/inward/record.url?scp=33644681191&partnerID=8YFLogxK
M3 - Article
C2 - 16334218
AN - SCOPUS:33644681191
SN - 0208-0613
SP - 9
EP - 14
JO - Molekuliarnaia genetika, mikrobiologiia i virusologiia
JF - Molekuliarnaia genetika, mikrobiologiia i virusologiia
IS - 4
ER -