Molecular epidemiology of Clostridioides (previously Clostridium) difficile isolates from a university hospital in Minas Gerais, Brazil

Amanda Nádia Diniz; Carlos Augusto de Oliveira Júnior; Eduardo Garcia Vilela; Henrique Cesar Pereira Figueiredo; Maja Rupnik; Mark H. Wilcox; Warren N. Fawley; Dominique S. Blanc; Francisco Carlos Faria Lobato; Rodrigo Otávio Silveira Silva

doi:10.1016/j.anaerobe.2019.01.010

Molecular epidemiology of Clostridioides (previously Clostridium) difficile isolates from a university hospital in Minas Gerais, Brazil

Amanda Nádia Diniz, Carlos Augusto de Oliveira Júnior, Eduardo Garcia Vilela, Henrique Cesar Pereira Figueiredo, Maja Rupnik, Mark H. Wilcox, Warren N. Fawley, Dominique S. Blanc, Francisco Carlos Faria Lobato, Rodrigo Otávio Silveira Silva^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

The molecular epidemiology of 38 non-duplicate toxigenic Clostridioides (previously Clostridium) difficile isolates from inpatients from a hospital in Brazil during a 6-year period (2012–2017) were investigated by multilocus sequence typing (MLST) and ribotyping. These isolates were classified into 20 sequence types (ST), six (30%) of which were novel, revealing a high diversity in a single hospital. Classic hypervirulent strains ST1/RT027 and ST11/RT078 were not identified, while ST42 (almost all RT106) was the most common type, being detected in 11 (28.9%) strains. Noteworthy, six (15.8%) isolates were classified into five STs from clade 2, four of which were new ST and RT. Our study suggests that possible hypervirulent strains other than ST1/RT027 might be inadvertently circulating in Brazilian hospitals and highlights the importance of permanent surveillance on circulating strains in a national scale.

Original language	English
Pages (from-to)	34-39
Number of pages	6
Journal	Anaerobe
Volume	56
DOIs	https://doi.org/10.1016/j.anaerobe.2019.01.010
Publication status	Published - Apr 2019
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2019 Elsevier Ltd

Keywords

Hypervirulent Clostridium difficile
Nosocomial diarrhea

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.anaerobe.2019.01.010

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Diniz, A. N., de Oliveira Júnior, C. A., Vilela, E. G., Figueiredo, H. C. P., Rupnik, M., Wilcox, M. H., Fawley, W. N., Blanc, D. S., Faria Lobato, F. C., & Silva, R. O. S. (2019). Molecular epidemiology of Clostridioides (previously Clostridium) difficile isolates from a university hospital in Minas Gerais, Brazil. Anaerobe, 56, 34-39. https://doi.org/10.1016/j.anaerobe.2019.01.010

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title = "Molecular epidemiology of Clostridioides (previously Clostridium) difficile isolates from a university hospital in Minas Gerais, Brazil",

abstract = "The molecular epidemiology of 38 non-duplicate toxigenic Clostridioides (previously Clostridium) difficile isolates from inpatients from a hospital in Brazil during a 6-year period (2012–2017) were investigated by multilocus sequence typing (MLST) and ribotyping. These isolates were classified into 20 sequence types (ST), six (30%) of which were novel, revealing a high diversity in a single hospital. Classic hypervirulent strains ST1/RT027 and ST11/RT078 were not identified, while ST42 (almost all RT106) was the most common type, being detected in 11 (28.9%) strains. Noteworthy, six (15.8%) isolates were classified into five STs from clade 2, four of which were new ST and RT. Our study suggests that possible hypervirulent strains other than ST1/RT027 might be inadvertently circulating in Brazilian hospitals and highlights the importance of permanent surveillance on circulating strains in a national scale.",

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year = "2019",

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doi = "10.1016/j.anaerobe.2019.01.010",

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AU - Vilela, Eduardo Garcia

AU - Figueiredo, Henrique Cesar Pereira

AU - Rupnik, Maja

AU - Wilcox, Mark H.

AU - Fawley, Warren N.

AU - Blanc, Dominique S.

AU - Faria Lobato, Francisco Carlos

AU - Silva, Rodrigo Otávio Silveira

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AB - The molecular epidemiology of 38 non-duplicate toxigenic Clostridioides (previously Clostridium) difficile isolates from inpatients from a hospital in Brazil during a 6-year period (2012–2017) were investigated by multilocus sequence typing (MLST) and ribotyping. These isolates were classified into 20 sequence types (ST), six (30%) of which were novel, revealing a high diversity in a single hospital. Classic hypervirulent strains ST1/RT027 and ST11/RT078 were not identified, while ST42 (almost all RT106) was the most common type, being detected in 11 (28.9%) strains. Noteworthy, six (15.8%) isolates were classified into five STs from clade 2, four of which were new ST and RT. Our study suggests that possible hypervirulent strains other than ST1/RT027 might be inadvertently circulating in Brazilian hospitals and highlights the importance of permanent surveillance on circulating strains in a national scale.

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Molecular epidemiology of Clostridioides (previously Clostridium) difficile isolates from a university hospital in Minas Gerais, Brazil

Abstract

Bibliographical note

Keywords

UN SDGs

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