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Molecular characterization of Salmonella Typhimurium and Salmonella Enteritidis by plasmid analysis and pulsed-field gel electrophoresis

  • Zerrin Aktas*
  • , Martin Day
  • , Cigdem Bal Kayacan
  • , Sukufe Diren
  • , E. John Threlfall
  • *Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    37 Citations (Scopus)

    Abstract

    Forty-one paediatric isolates of Salmonella spp. from Cerrahpasa Faculty of Medicine, Istanbul, Turkey, between 2001 and 2004 were examined for susceptibility to various antibiotics and presence of antibiotic resistance genes. Pulsed-field gel electrophoresis and plasmid profiling were used to determine possible genetic relationships among Salmonella enterica subsp. enterica clinical isolates. Plasmids from resistant strains were not transferred by conjugation to recipient Escherichia coli cells. Pulsed-field gel electrophoresis and restriction enzyme digestion analysis of DNA revealed that multidrug-resistant isolates belonged to the same clonal group, characterized by ACSSuT resistotype. Isolates of R-type ACSSuT were positive for the intI gene and possessed a single plasmid of 60 MDa.

    Original languageEnglish
    Pages (from-to)541-545
    Number of pages5
    JournalInternational Journal of Antimicrobial Agents
    Volume30
    Issue number6
    DOIs
    Publication statusPublished - Dec 2007

    Bibliographical note

    Funding Information:
    Funding: This study was supported by the Research Fund of the Istanbul University, project number UDP-516/15022007.

    UN SDGs

    This output contributes to the following UN Sustainable Development Goals (SDGs)

    1. SDG 3 - Good Health and Well-being
      SDG 3 Good Health and Well-being

    Keywords

    • ACSSuT
    • Plasmid profile analysis
    • Salmonella Enteritidis
    • Salmonella Typhimurium DT 104

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