Molecular analysis of human group A rotavirus G10P[14] genotype in Slovenia

A. Steyer; Mojca Bajželj; Miren Iturriza-Go'Mara; Zornitsa Mladenova; Neli Korsun; Mateja Poljšak-Prijatelj

doi:10.1016/j.jcv.2010.07.003

Molecular analysis of human group A rotavirus G10P[14] genotype in Slovenia

A. Steyer^*
, Mojca Bajželj
, Miren Iturriza-Go'Mara
, Zornitsa Mladenova
, Neli Korsun
, Mateja Poljšak-Prijatelj

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

Background:Rotavirus G10 genotype is one of the main rotaviruses circulating in cattle throughout the world but is also found in asymptomatic and symptomatic infections in children, and thought to be acquired through zoonotic transmission. Objectives: To determine the genetic diversity of G10P[14] rotavirus strains detected in various regions in Slovenia during a study on the molecular epidemiology of rotaviruses conducted in 2007. Study design: Five G10P[14] rotavirus strains detected in Slovenia in 2007 were subjected to sequencing and phylogenetic analysis of the genes encoding VP7, NSP4 and partial VP4 (VP8*) and VP6 rotavirus proteins. Results: Sequence and phylogenetic analysis of the four genes analyzed revealed a significant genetic diversity. Overall, the Slovenian G10P[14] are divided into two phylogenetic lineages. Conclusions: These results suggest that the G10P[14] strains found in Slovenian children did not emerge from a common source but possibly result of at least two independent zoonotic transmissions. Phylogenetic analysis and comparison with sequence data available in GenBank points towards a bovine origin to these strains.

Original language	English
Pages (from-to)	121-125
Number of pages	5
Journal	Journal of Clinical Virology
Volume	49
Issue number	2
DOIs	https://doi.org/10.1016/j.jcv.2010.07.003
Publication status	Published - Oct 2010

Bibliographical note

Funding Information:
This study was partially supported by the Slovenian Research Agency (post-doc grant Z3-0037-0381-08 and Slovenian–Bulgarian bilateral project SI-BG/09-10-014) and EuroRotaNet, funded by GlaxoSmithKline and Sanofi Pasteur-MSD.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

EuroRotaNet
G10P[14] genotype
Rotavirus
Slovenia
Zoonotic transmission

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10.1016/j.jcv.2010.07.003

Cite this

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title = "Molecular analysis of human group A rotavirus G10P[14] genotype in Slovenia",

abstract = "Background:Rotavirus G10 genotype is one of the main rotaviruses circulating in cattle throughout the world but is also found in asymptomatic and symptomatic infections in children, and thought to be acquired through zoonotic transmission. Objectives: To determine the genetic diversity of G10P[14] rotavirus strains detected in various regions in Slovenia during a study on the molecular epidemiology of rotaviruses conducted in 2007. Study design: Five G10P[14] rotavirus strains detected in Slovenia in 2007 were subjected to sequencing and phylogenetic analysis of the genes encoding VP7, NSP4 and partial VP4 (VP8*) and VP6 rotavirus proteins. Results: Sequence and phylogenetic analysis of the four genes analyzed revealed a significant genetic diversity. Overall, the Slovenian G10P[14] are divided into two phylogenetic lineages. Conclusions: These results suggest that the G10P[14] strains found in Slovenian children did not emerge from a common source but possibly result of at least two independent zoonotic transmissions. Phylogenetic analysis and comparison with sequence data available in GenBank points towards a bovine origin to these strains.",

keywords = "EuroRotaNet, G10P[14] genotype, Rotavirus, Slovenia, Zoonotic transmission",

author = "A. Steyer and Mojca Baj{\v z}elj and Miren Iturriza-Go'Mara and Zornitsa Mladenova and Neli Korsun and Mateja Polj{\v s}ak-Prijatelj",

note = "Funding Information: This study was partially supported by the Slovenian Research Agency (post-doc grant Z3-0037-0381-08 and Slovenian–Bulgarian bilateral project SI-BG/09-10-014) and EuroRotaNet, funded by GlaxoSmithKline and Sanofi Pasteur-MSD. ",

year = "2010",

month = oct,

doi = "10.1016/j.jcv.2010.07.003",

language = "English",

volume = "49",

pages = "121--125",

journal = "Journal of Clinical Virology",

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T1 - Molecular analysis of human group A rotavirus G10P[14] genotype in Slovenia

AU - Steyer, A.

AU - Bajželj, Mojca

AU - Iturriza-Go'Mara, Miren

AU - Mladenova, Zornitsa

AU - Korsun, Neli

AU - Poljšak-Prijatelj, Mateja

N1 - Funding Information: This study was partially supported by the Slovenian Research Agency (post-doc grant Z3-0037-0381-08 and Slovenian–Bulgarian bilateral project SI-BG/09-10-014) and EuroRotaNet, funded by GlaxoSmithKline and Sanofi Pasteur-MSD.

PY - 2010/10

Y1 - 2010/10

N2 - Background:Rotavirus G10 genotype is one of the main rotaviruses circulating in cattle throughout the world but is also found in asymptomatic and symptomatic infections in children, and thought to be acquired through zoonotic transmission. Objectives: To determine the genetic diversity of G10P[14] rotavirus strains detected in various regions in Slovenia during a study on the molecular epidemiology of rotaviruses conducted in 2007. Study design: Five G10P[14] rotavirus strains detected in Slovenia in 2007 were subjected to sequencing and phylogenetic analysis of the genes encoding VP7, NSP4 and partial VP4 (VP8*) and VP6 rotavirus proteins. Results: Sequence and phylogenetic analysis of the four genes analyzed revealed a significant genetic diversity. Overall, the Slovenian G10P[14] are divided into two phylogenetic lineages. Conclusions: These results suggest that the G10P[14] strains found in Slovenian children did not emerge from a common source but possibly result of at least two independent zoonotic transmissions. Phylogenetic analysis and comparison with sequence data available in GenBank points towards a bovine origin to these strains.

AB - Background:Rotavirus G10 genotype is one of the main rotaviruses circulating in cattle throughout the world but is also found in asymptomatic and symptomatic infections in children, and thought to be acquired through zoonotic transmission. Objectives: To determine the genetic diversity of G10P[14] rotavirus strains detected in various regions in Slovenia during a study on the molecular epidemiology of rotaviruses conducted in 2007. Study design: Five G10P[14] rotavirus strains detected in Slovenia in 2007 were subjected to sequencing and phylogenetic analysis of the genes encoding VP7, NSP4 and partial VP4 (VP8*) and VP6 rotavirus proteins. Results: Sequence and phylogenetic analysis of the four genes analyzed revealed a significant genetic diversity. Overall, the Slovenian G10P[14] are divided into two phylogenetic lineages. Conclusions: These results suggest that the G10P[14] strains found in Slovenian children did not emerge from a common source but possibly result of at least two independent zoonotic transmissions. Phylogenetic analysis and comparison with sequence data available in GenBank points towards a bovine origin to these strains.

KW - EuroRotaNet

KW - G10P[14] genotype

KW - Rotavirus

KW - Slovenia

KW - Zoonotic transmission

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SN - 1386-6532

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JO - Journal of Clinical Virology

JF - Journal of Clinical Virology

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ER -

Molecular analysis of human group A rotavirus G10P[14] genotype in Slovenia

Abstract

Bibliographical note

UN SDGs

Keywords

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