Measles immunity testing: Comparison of two measles IgG ELISAs with plaque reduction neutralisation assay

B. J. Cohen; R. P. Parry; D. Doblas; Dhanraj Samuel; L. Warrener; Nicholas Andrews; David Brown

doi:10.1016/j.jviromet.2005.08.001

Measles immunity testing: Comparison of two measles IgG ELISAs with plaque reduction neutralisation assay

B. J. Cohen^*, R. P. Parry, D. Doblas, Dhanraj Samuel, L. Warrener, Nicholas Andrews, David Brown

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

78 Citations (Scopus)

Abstract

Two commercial IgG ELISAs, one based on recombinant nucleocapsid antigen and one based on cell culture grown native virus antigens, were evaluated for measles immunity testing by comparison with plaque reduction neutralisation test (PRNT). Qualitative results of the two ELISAs showed 92% agreement with those of PRNT. The sensitivity of the two ELISAs was 89.6%. False negative ELISA results were obtained in 10% of sera, mainly sera containing low levels of neutralising antibody. The specificity of both ELISAs was 100%. Measles IgG ELISAs perform adequately for immunity testing, correctly identifying seronegative individuals for vaccination.

Original language	English
Pages (from-to)	209-212
Number of pages	4
Journal	Journal of Virological Methods
Volume	131
Issue number	2
DOIs	https://doi.org/10.1016/j.jviromet.2005.08.001
Publication status	Published - Feb 2006

Keywords

ELISA
Immunity
Measles
Neutralisation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jviromet.2005.08.001

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title = "Measles immunity testing: Comparison of two measles IgG ELISAs with plaque reduction neutralisation assay",

abstract = "Two commercial IgG ELISAs, one based on recombinant nucleocapsid antigen and one based on cell culture grown native virus antigens, were evaluated for measles immunity testing by comparison with plaque reduction neutralisation test (PRNT). Qualitative results of the two ELISAs showed 92% agreement with those of PRNT. The sensitivity of the two ELISAs was 89.6%. False negative ELISA results were obtained in 10% of sera, mainly sera containing low levels of neutralising antibody. The specificity of both ELISAs was 100%. Measles IgG ELISAs perform adequately for immunity testing, correctly identifying seronegative individuals for vaccination.",

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AU - Cohen, B. J.

AU - Parry, R. P.

AU - Doblas, D.

AU - Samuel, Dhanraj

AU - Warrener, L.

AU - Andrews, Nicholas

AU - Brown, David

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AB - Two commercial IgG ELISAs, one based on recombinant nucleocapsid antigen and one based on cell culture grown native virus antigens, were evaluated for measles immunity testing by comparison with plaque reduction neutralisation test (PRNT). Qualitative results of the two ELISAs showed 92% agreement with those of PRNT. The sensitivity of the two ELISAs was 89.6%. False negative ELISA results were obtained in 10% of sera, mainly sera containing low levels of neutralising antibody. The specificity of both ELISAs was 100%. Measles IgG ELISAs perform adequately for immunity testing, correctly identifying seronegative individuals for vaccination.

KW - ELISA

KW - Immunity

KW - Measles

KW - Neutralisation

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Measles immunity testing: Comparison of two measles IgG ELISAs with plaque reduction neutralisation assay

Abstract

Keywords

UN SDGs

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