Mass Spectral Analysis of Proteins of Nonculture and Cultured Viruses

Vlad Serafim*, Nicola Hennessy, David J. Allen, Christopher Ring, Leonardo P. Munoz, Saheer Gharbia, Ajit J. Shah, Haroun N. Shah

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Nucleic-acid-based matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) applications for the detection and identification of viruses use specific or broad-range polymerase chain reaction (PCR) primers in combination with different post-PCR biochemistries. The most widely used biochemistry and variations thereof, a PCR/primer extension assay has been applied to the detection and genotyping of RNA and DNA viruses with a multiplexing level and throughput that support large-scale epidemiological research studies and might improve diagnosis of infections and co-infections in clinical applications. The study demonstrates the importance of genetic analysis and informative molecular markers for rapid and high-throughput epidemiological studies of pathogen transmission. In addition to the detection of viruses, PCR/primer extension assays on MALDI-TOF MS enable the characterization of viruses by genotyping. PCR/primer extension assays have successfully been applied to genotype Hepatitis B virus (HBV) and to detect up to 60 single-nucleotide drug resistance mutations in the HBV polymerase gene.

Original languageEnglish
Title of host publicationMALDI-TOF and Tandem MS for Clinical Microbiology
Publisherwiley
Pages197-209
Number of pages13
ISBN (Electronic)9781118960226
ISBN (Print)9781118960257
DOIs
Publication statusPublished - 15 Sep 2016

Keywords

  • Hepatitis B virus
  • MALDI-TOF MS
  • Microbial DNA analysis
  • Molecular detection
  • Polymerase chain reaction
  • Primer extension assays
  • Tandem MS
  • Viral genetic heterogeneity
  • Viral quantification
  • Viral transmission monitoring

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