Mapping of the Proteogenome of Clostridium difficile Isolates of Varying Virulence

Caroline H. Chilton*, Saheer Gharbia, Raju V. Misra, Min Fang, Ian R. Poxton, Peter S. Borriello, Haroun N. Shah

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

This chapter explores the use of genomics and proteomics to investigate factors responsible for the varying virulence of different Clostridium difficile strains. At first glance, the virulence of C. difficile is a simple process, in that disease is mediated by two large clostridial toxins, toxin A and toxin B. In order to maximize the power of genomic and proteomic data in the elucidation of factors responsible for C. difficile virulence, the authors performed tandem genomic and proteomic analysis on C. difficile strains exhibiting different virulence profiles. They then compared the genomic and proteomic profiles to known phenotypic data for these strains in an attempt to identify factors that could explain the differences in virulence. The chapter discusses some aspects of virulence and how proteogenome mapping has highlighted strain-to-strain differences, and gives some insight into how these differences may be linked to virulence.

Original languageEnglish
Title of host publicationMALDI-TOF and Tandem MS for Clinical Microbiology
Publisherwiley
Pages379-398
Number of pages20
ISBN (Electronic)9781118960226
ISBN (Print)9781118960257
DOIs
Publication statusPublished - 15 Sep 2016

Keywords

  • Clostridial toxins
  • Clostridium difficile isolates
  • Clostridium difficile virulence
  • Proteogenome mapping
  • Strain-to-strain differences
  • Tandem genomic analysis
  • Tandem proteomic analysis

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