Liseq – Whole-genome sequencing of a cross-sectional survey of listeria monocytogenes in ready-to-eat foods and human clinical cases in Europe

Anaïs Painset; Jonas T. Björkman; Kristoffer Kiil; Laurent Guillier; Jean François Mariet; Benjamin Felix; Corinne Amar; Ovidiu Rotariu; Sophie Roussel; Francisco Perez-Reche; Sylvain Brisse; Alexandra Moura; Marc Lecuit; Ken Forbes; Norval Strachan; Kathie Grant; Eva Møller-Nielsen; Timothy J. Dallman

doi:10.1099/mgen.0.000257

Liseq – Whole-genome sequencing of a cross-sectional survey of listeria monocytogenes in ready-to-eat foods and human clinical cases in Europe

Anaïs Painset, Jonas T. Björkman, Kristoffer Kiil, Laurent Guillier, Jean François Mariet, Benjamin Felix, Corinne Amar, Ovidiu Rotariu, Sophie Roussel, Francisco Perez-Reche, Sylvain Brisse, Alexandra Moura, Marc Lecuit, Ken Forbes, Norval Strachan, Kathie Grant, Eva Møller-Nielsen, Timothy J. Dallman^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

98 Citations (Scopus)

Abstract

We present the LiSEQ (Listeria SEQuencing) project, funded by the European Food Safety Agency (EFSA) to compare Listeria monocytogenes isolates collected in the European Union from ready-to-eat foods, compartments along the food chain (e.g. food-producing animals, food-processing environments) and humans. In this article, we report the molecular characterization of a selection of this data set employing whole-genome sequencing analysis. We present an overview of the strain diversity observed in different sampled sources, and characterize the isolates based on their virulence and resistance profile. We integrate into our analysis the global L. monocytogenes genome collection described by Moura and colleagues in 2016 to assess the representativeness of the LiSEQ collection in the context of known L. monocytogenes strain diversity.

Original language	English
Article number	000257
Journal	Microbial Genomics
Volume	5
Issue number	2
DOIs	https://doi.org/10.1099/mgen.0.000257
Publication status	Published - Feb 2019

Bibliographical note

Publisher Copyright:
© 2019 The Authors.

Keywords

Food
Genetic diversity
Human
Listeria monocytogenes
Phylogeny
Whole-genome sequencing

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10.1099/mgen.0.000257

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Painset, A., Björkman, J. T., Kiil, K., Guillier, L., Mariet, J. F., Felix, B., Amar, C., Rotariu, O., Roussel, S., Perez-Reche, F., Brisse, S., Moura, A., Lecuit, M., Forbes, K., Strachan, N., Grant, K., Møller-Nielsen, E., & Dallman, T. J. (2019). Liseq – Whole-genome sequencing of a cross-sectional survey of listeria monocytogenes in ready-to-eat foods and human clinical cases in Europe. Microbial Genomics, 5(2), Article 000257. https://doi.org/10.1099/mgen.0.000257

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abstract = "We present the LiSEQ (Listeria SEQuencing) project, funded by the European Food Safety Agency (EFSA) to compare Listeria monocytogenes isolates collected in the European Union from ready-to-eat foods, compartments along the food chain (e.g. food-producing animals, food-processing environments) and humans. In this article, we report the molecular characterization of a selection of this data set employing whole-genome sequencing analysis. We present an overview of the strain diversity observed in different sampled sources, and characterize the isolates based on their virulence and resistance profile. We integrate into our analysis the global L. monocytogenes genome collection described by Moura and colleagues in 2016 to assess the representativeness of the LiSEQ collection in the context of known L. monocytogenes strain diversity.",

keywords = "Food, Genetic diversity, Human, Listeria monocytogenes, Phylogeny, Whole-genome sequencing",

author = "Ana{\"i}s Painset and Bj{\"o}rkman, {Jonas T.} and Kristoffer Kiil and Laurent Guillier and Mariet, {Jean Fran{\c c}ois} and Benjamin Felix and Corinne Amar and Ovidiu Rotariu and Sophie Roussel and Francisco Perez-Reche and Sylvain Brisse and Alexandra Moura and Marc Lecuit and Ken Forbes and Norval Strachan and Kathie Grant and Eva M{\o}ller-Nielsen and Dallman, {Timothy J.}",

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doi = "10.1099/mgen.0.000257",

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Painset, A, Björkman, JT, Kiil, K, Guillier, L, Mariet, JF, Felix, B, Amar, C, Rotariu, O, Roussel, S, Perez-Reche, F, Brisse, S, Moura, A, Lecuit, M, Forbes, K, Strachan, N, Grant, K, Møller-Nielsen, E & Dallman, TJ 2019, 'Liseq – Whole-genome sequencing of a cross-sectional survey of listeria monocytogenes in ready-to-eat foods and human clinical cases in Europe', Microbial Genomics, vol. 5, no. 2, 000257. https://doi.org/10.1099/mgen.0.000257

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AU - Björkman, Jonas T.

AU - Kiil, Kristoffer

AU - Guillier, Laurent

AU - Mariet, Jean François

AU - Felix, Benjamin

AU - Amar, Corinne

AU - Rotariu, Ovidiu

AU - Roussel, Sophie

AU - Perez-Reche, Francisco

AU - Brisse, Sylvain

AU - Moura, Alexandra

AU - Lecuit, Marc

AU - Forbes, Ken

AU - Strachan, Norval

AU - Grant, Kathie

AU - Møller-Nielsen, Eva

AU - Dallman, Timothy J.

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AB - We present the LiSEQ (Listeria SEQuencing) project, funded by the European Food Safety Agency (EFSA) to compare Listeria monocytogenes isolates collected in the European Union from ready-to-eat foods, compartments along the food chain (e.g. food-producing animals, food-processing environments) and humans. In this article, we report the molecular characterization of a selection of this data set employing whole-genome sequencing analysis. We present an overview of the strain diversity observed in different sampled sources, and characterize the isolates based on their virulence and resistance profile. We integrate into our analysis the global L. monocytogenes genome collection described by Moura and colleagues in 2016 to assess the representativeness of the LiSEQ collection in the context of known L. monocytogenes strain diversity.

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KW - Genetic diversity

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ER -

Liseq – Whole-genome sequencing of a cross-sectional survey of listeria monocytogenes in ready-to-eat foods and human clinical cases in Europe

Abstract

Bibliographical note

Keywords

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