Lens Epithelial Cell Proliferation in Response to Ionizing Radiation

S. Barnard, A. Uwineza, A. Kalligeraki, R. McCarron, F. Kruse, E. A. Ainsbury, R. A. Quinlan

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Lens epithelial cell proliferation and differentiation are naturally well regulated and controlled, a characteristic essential for lens structure, symmetry and function. The effect of ionizing radiation on lens epithelial cell proliferation has been demonstrated in previous studies at high acute doses, but the effect of dose and dose rate on proliferation has not yet been considered. In this work, mice received single acute doses of 0.5, 1 and 2 Gy of radiation, at dose rates of 0.063 and 0.3 Gy/min. Eye lenses were isolated postirradiation at 30 min up until 14 days and flat-mounted. Then, cell proliferation rates were determined using biomarker Ki67. As expected, radiation increased cell proliferation 2 and 24 h postirradiation transiently (undetectable 14 days postirradiation) and was dose dependent (changes were very significant at 2 Gy; P = 0.008). A dose-rate effect did not reach significance in this study (P = 0.054). However, dose rate and lens epithelial cell region showed significant interactions (P < 0.001). These observations further our mechanistic understanding of how the lens responds to radiation.

Original languageEnglish
Pages (from-to)92-99
Number of pages8
JournalRadiation Research
Volume197
Issue number1
Early online date13 May 2021
DOIs
Publication statusPublished - 1 Jan 2022

Bibliographical note

Funding information: The LDLensRad project has received funding from the Euratom Research and Training Programme 2014–2018 in the framework of the CONCERT
(grant agreement no. 662287).

Open Access: Free to read, but no Open Access licence.

Publisher Copyright: ©2022 by Radiation Research Society. All rights of reproduction in any form reserved.

Citation: S. Barnard, A. Uwineza, A. Kalligeraki, R. McCarron, F. Kruse, E. A. Ainsbury, and R. A. Quinlan "Lens Epithelial Cell Proliferation in Response to Ionizing Radiation," Radiation Research 197(1), 92-99, (13 May 2021).

DOI: https://doi.org/10.1667/RADE-20-00294.1

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