Laboratory methods for assessing vaccine potency retained in aerosol outputs from nebulizers: Application to World Health Organization measles aerosol project

B. J. Cohen*, R. P. Parry, N. Andrews, A. M. Bennett, J. H. Dennis

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Background: Laboratory methods for measuring vaccine potency of nebulized aerosol are required to support clinical trials of measles aerosol vaccination. Methods: Measles vaccine containing the Edmonston Zagreb virus strain was reconstituted in sodium fluoride as tracer and nebulized from three devices. Emitted aerosol was collected using an impinger. Aliquots were removed from the impinger chamber for vaccine virus plaque assay and for fluoride measurement to determine aerosol output. Results: Vaccine potency retention results were adjusted to take into account the effect of aerosol output on estimates. Adjusted potency of nebulized vaccine ranged from 88% to 102%. Conclusions: New laboratory methods to measure aerosol vaccine potency retention were reliable and accurate. The results demonstrated that Edmonston Zagreb vaccine remains robust during aerosolisation and imply that this is a viable candidate for further evaluation in the measles aerosol project.

Original languageEnglish
Pages (from-to)3534-3539
Number of pages6
JournalVaccine
Volume26
Issue number27-28
DOIs
Publication statusPublished - 25 Jun 2008

Bibliographical note

Funding Information:
The authors alone are responsible for the views expressed in this manuscript and they do not necessarily represent the decisions, policy or views of the World Health Organization. World Health Organization Initiative for Vaccine Research (WHO/IVR) co-ordinates the Measles Aerosol Project (MAP). The Centre for Disease Control and Prevention (CDC), USA and, the American Red Cross (ARC) are partners in MAP, which has received financial support from the Bill and Melinda Gates Foundation. We thank Dr Ana Maria Henao Restrepo, focal point for MAP at WHO/IVR, for encouraging this work, Dr David Brown of the Health Protection Agency Centre for Infections (CfI) for advice and Mr Brian Megson of the CfI Electron Microscopy Unit for performing electron microscopy on vaccine samples.

Keywords

  • Aerosol
  • Measles vaccine
  • Nebulizer
  • Potency retention

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