Klebsiella pneumoniae isolate from South Africa with multiple TEM, SHV and AmpC β-lactamases

Sabiha Y. Essack; Lucinda M.C. Hall; David Livermore

doi:10.1016/j.ijantimicag.2003.08.010

Klebsiella pneumoniae isolate from South Africa with multiple TEM, SHV and AmpC β-lactamases

Sabiha Y. Essack, Lucinda M.C. Hall, David Livermore^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Klebsiella pneumoniae 2207, from Durban, was resistant to cefoxitin and β-lactamase inhibitor combinations as well as oxyimino-aminothiazolyl cephalosporins. β-Lactamases with isoelectric points of 5.4, 5.6, 7.6, 8.2 and 8.4 were found. DNA hybridisation identified two BamHI and three HindIII fragments carrying bla_TEM, and two SalI fragments carrying bla _SHV. At least two genes encoded TEM-1 enzyme; one bla_SHV copy encoded SHV-5 but the other determined SHV-23, a novel SHV-5 variant with conservative amino-acid substitutions far from the catalytic site. The pI 8.4 activity was an AmpC-type enzyme. Determinants of the pI 5.6 and 7.6 activities were not identified.

Original language	English
Pages (from-to)	398-400
Number of pages	3
Journal	International Journal of Antimicrobial Agents
Volume	23
Issue number	4
DOIs	https://doi.org/10.1016/j.ijantimicag.2003.08.010
Publication status	Published - Apr 2004

Bibliographical note

Funding Information:
Sabiha Essack is grateful to the Wellcome Trust for a Travelling Fellowship (Ref. No. 048863/Z/96/Z).

Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.

Keywords

AmpC β-lactamase
Extended-spectrum β-lactamases
K. pneumoniae

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ijantimicag.2003.08.010

Cite this

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title = "Klebsiella pneumoniae isolate from South Africa with multiple TEM, SHV and AmpC β-lactamases",

abstract = "Klebsiella pneumoniae 2207, from Durban, was resistant to cefoxitin and β-lactamase inhibitor combinations as well as oxyimino-aminothiazolyl cephalosporins. β-Lactamases with isoelectric points of 5.4, 5.6, 7.6, 8.2 and 8.4 were found. DNA hybridisation identified two BamHI and three HindIII fragments carrying blaTEM, and two SalI fragments carrying bla SHV. At least two genes encoded TEM-1 enzyme; one blaSHV copy encoded SHV-5 but the other determined SHV-23, a novel SHV-5 variant with conservative amino-acid substitutions far from the catalytic site. The pI 8.4 activity was an AmpC-type enzyme. Determinants of the pI 5.6 and 7.6 activities were not identified.",

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AU - Essack, Sabiha Y.

AU - Hall, Lucinda M.C.

AU - Livermore, David

PY - 2004/4

Y1 - 2004/4

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AB - Klebsiella pneumoniae 2207, from Durban, was resistant to cefoxitin and β-lactamase inhibitor combinations as well as oxyimino-aminothiazolyl cephalosporins. β-Lactamases with isoelectric points of 5.4, 5.6, 7.6, 8.2 and 8.4 were found. DNA hybridisation identified two BamHI and three HindIII fragments carrying blaTEM, and two SalI fragments carrying bla SHV. At least two genes encoded TEM-1 enzyme; one blaSHV copy encoded SHV-5 but the other determined SHV-23, a novel SHV-5 variant with conservative amino-acid substitutions far from the catalytic site. The pI 8.4 activity was an AmpC-type enzyme. Determinants of the pI 5.6 and 7.6 activities were not identified.

KW - AmpC β-lactamase

KW - Extended-spectrum β-lactamases

KW - K. pneumoniae

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ER -

Klebsiella pneumoniae isolate from South Africa with multiple TEM, SHV and AmpC β-lactamases

Abstract

Bibliographical note

Keywords

UN SDGs

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