Some Kelch mutations of the Plasmodium falciparum K13 protein confer increased survival to dihydroartemisinin (DHA)-treated ring-stage parasites. Here, we asked if K13 mutations affect a dormancy phenotype allowing parasites to survive DHA exposure and then sorbitol selection. Although recrudescence from dormancy differed between two distinct parasite lines, it was similar for isogenic lines carrying single-site substitutions in K13. Therefore, K13 mutations do not alter the DHA-sorbitol combined dormancy phenotype; rather, traits from other loci likely determine this phenotype.
Bibliographical noteFunding Information:
We thank Judith Straimer and David Fidock for providing the edited 967 parasite clones, and Katja Simon, Craig J. Thomas, and Rick Fairhurst for discussions. This work was supported by the NIH Oxford Scholars Program and by the Intramural Research Program of the National Institute of Allergy and Infectious Diseases. We declare no conflicts of interest.
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- Drug resistance