Isolation of oropouche virus from febrile patient, Ecuador

Emma L. Wise; Steven Pullan; Sully Márquez; Verónica Paz; Juan D. Mosquera; Sonia Zapata; Simon K. Jackson; Gyorgy Fejer; Gabriel Trueba; Christopher H. Logue

doi:10.3201/eid2405.171569

Isolation of oropouche virus from febrile patient, Ecuador

Emma L. Wise^*
, Steven Pullan
, Sully Márquez
, Verónica Paz
, Juan D. Mosquera
, Sonia Zapata
, Simon K. Jackson
, Gyorgy Fejer
, Gabriel Trueba
, Christopher H. Logue

^*Corresponding author for this work

Countermeasures Development & Evaluation Project Staff

Research output: Contribution to journal › Letter › peer-review

28 Citations (Scopus)

Abstract

We report identification of an Oropouche virus strain in a febrile patient from Ecuador by using metagenomic sequencing and real-time reverse transcription PCR. Virus was isolated from patient serum by using Vero cells. Phylogenetic analysis of the whole-genome sequence showed the virus to be similar to a strain from Peru.

Original language	English
Pages (from-to)	935-937
Number of pages	3
Journal	Emerging Infectious Diseases
Volume	24
Issue number	5
DOIs	https://doi.org/10.3201/eid2405.171569
Publication status	Published - May 2018

Bibliographical note

Funding Information:
Ms. Wise is a PhD student registered with Plymouth University and funded by Public Health England. Her research interests are emerging viral infections and viral immunology.

Publisher Copyright:
© 2018, Centers for Disease Control and Prevention (CDC). All rights reserved.

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.3201/eid2405.171569

Cite this

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title = "Isolation of oropouche virus from febrile patient, Ecuador",

abstract = "We report identification of an Oropouche virus strain in a febrile patient from Ecuador by using metagenomic sequencing and real-time reverse transcription PCR. Virus was isolated from patient serum by using Vero cells. Phylogenetic analysis of the whole-genome sequence showed the virus to be similar to a strain from Peru.",

author = "Wise, \{Emma L.\} and Steven Pullan and Sully M{\'a}rquez and Ver{\'o}nica Paz and Mosquera, \{Juan D.\} and Sonia Zapata and Jackson, \{Simon K.\} and Gyorgy Fejer and Gabriel Trueba and Logue, \{Christopher H.\}",

note = "Funding Information: Ms. Wise is a PhD student registered with Plymouth University and funded by Public Health England. Her research interests are emerging viral infections and viral immunology. Publisher Copyright: {\textcopyright} 2018, Centers for Disease Control and Prevention (CDC). All rights reserved.",

year = "2018",

month = may,

doi = "10.3201/eid2405.171569",

language = "English",

volume = "24",

pages = "935--937",

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T1 - Isolation of oropouche virus from febrile patient, Ecuador

AU - Wise, Emma L.

AU - Pullan, Steven

AU - Márquez, Sully

AU - Paz, Verónica

AU - Mosquera, Juan D.

AU - Zapata, Sonia

AU - Jackson, Simon K.

AU - Fejer, Gyorgy

AU - Trueba, Gabriel

AU - Logue, Christopher H.

N1 - Funding Information: Ms. Wise is a PhD student registered with Plymouth University and funded by Public Health England. Her research interests are emerging viral infections and viral immunology. Publisher Copyright: © 2018, Centers for Disease Control and Prevention (CDC). All rights reserved.

PY - 2018/5

Y1 - 2018/5

N2 - We report identification of an Oropouche virus strain in a febrile patient from Ecuador by using metagenomic sequencing and real-time reverse transcription PCR. Virus was isolated from patient serum by using Vero cells. Phylogenetic analysis of the whole-genome sequence showed the virus to be similar to a strain from Peru.

AB - We report identification of an Oropouche virus strain in a febrile patient from Ecuador by using metagenomic sequencing and real-time reverse transcription PCR. Virus was isolated from patient serum by using Vero cells. Phylogenetic analysis of the whole-genome sequence showed the virus to be similar to a strain from Peru.

UR - https://www.scopus.com/pages/publications/85045647979

U2 - 10.3201/eid2405.171569

DO - 10.3201/eid2405.171569

M3 - Letter

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AN - SCOPUS:85045647979

SN - 1080-6040

VL - 24

SP - 935

EP - 937

JO - Emerging Infectious Diseases

JF - Emerging Infectious Diseases

IS - 5

ER -

Isolation of oropouche virus from febrile patient, Ecuador

Abstract

Bibliographical note

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