Isolation of oropouche virus from febrile patient, Ecuador

Emma L. Wise; Steven Pullan; Sully Márquez; Verónica Paz; Juan D. Mosquera; Sonia Zapata; Simon K. Jackson; Gyorgy Fejer; Gabriel Trueba; Christopher H. Logue

doi:10.3201/eid2405.171569

Isolation of oropouche virus from febrile patient, Ecuador

Emma L. Wise^*, Steven Pullan, Sully Márquez, Verónica Paz, Juan D. Mosquera, Sonia Zapata, Simon K. Jackson, Gyorgy Fejer, Gabriel Trueba, Christopher H. Logue

^*Corresponding author for this work

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Research output: Contribution to journal › Letter › peer-review

23 Citations (Scopus)

Abstract

We report identification of an Oropouche virus strain in a febrile patient from Ecuador by using metagenomic sequencing and real-time reverse transcription PCR. Virus was isolated from patient serum by using Vero cells. Phylogenetic analysis of the whole-genome sequence showed the virus to be similar to a strain from Peru.

Original language	English
Pages (from-to)	935-937
Number of pages	3
Journal	Emerging Infectious Diseases
Volume	24
Issue number	5
DOIs	https://doi.org/10.3201/eid2405.171569
Publication status	Published - May 2018

Bibliographical note

Funding Information:
Ms. Wise is a PhD student registered with Plymouth University and funded by Public Health England. Her research interests are emerging viral infections and viral immunology.

Publisher Copyright:
© 2018, Centers for Disease Control and Prevention (CDC). All rights reserved.

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10.3201/eid2405.171569

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title = "Isolation of oropouche virus from febrile patient, Ecuador",

abstract = "We report identification of an Oropouche virus strain in a febrile patient from Ecuador by using metagenomic sequencing and real-time reverse transcription PCR. Virus was isolated from patient serum by using Vero cells. Phylogenetic analysis of the whole-genome sequence showed the virus to be similar to a strain from Peru.",

author = "Wise, {Emma L.} and Steven Pullan and Sully M{\'a}rquez and Ver{\'o}nica Paz and Mosquera, {Juan D.} and Sonia Zapata and Jackson, {Simon K.} and Gyorgy Fejer and Gabriel Trueba and Logue, {Christopher H.}",

note = "Funding Information: Ms. Wise is a PhD student registered with Plymouth University and funded by Public Health England. Her research interests are emerging viral infections and viral immunology. Publisher Copyright: {\textcopyright} 2018, Centers for Disease Control and Prevention (CDC). All rights reserved.",

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AU - Wise, Emma L.

AU - Pullan, Steven

AU - Márquez, Sully

AU - Paz, Verónica

AU - Mosquera, Juan D.

AU - Zapata, Sonia

AU - Jackson, Simon K.

AU - Fejer, Gyorgy

AU - Trueba, Gabriel

AU - Logue, Christopher H.

N1 - Funding Information: Ms. Wise is a PhD student registered with Plymouth University and funded by Public Health England. Her research interests are emerging viral infections and viral immunology. Publisher Copyright: © 2018, Centers for Disease Control and Prevention (CDC). All rights reserved.

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N2 - We report identification of an Oropouche virus strain in a febrile patient from Ecuador by using metagenomic sequencing and real-time reverse transcription PCR. Virus was isolated from patient serum by using Vero cells. Phylogenetic analysis of the whole-genome sequence showed the virus to be similar to a strain from Peru.

AB - We report identification of an Oropouche virus strain in a febrile patient from Ecuador by using metagenomic sequencing and real-time reverse transcription PCR. Virus was isolated from patient serum by using Vero cells. Phylogenetic analysis of the whole-genome sequence showed the virus to be similar to a strain from Peru.

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ER -

Isolation of oropouche virus from febrile patient, Ecuador

Abstract

Bibliographical note

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