Isolation of five different primary cell types from a single sample of human skin

Sylwia Kabacik; Donna Lowe; Howard Cohen; Sarah Felton; Joseph Spitzer; Ken Raj

doi:10.1016/j.xpro.2022.101378

Isolation of five different primary cell types from a single sample of human skin

Sylwia Kabacik^*, Donna Lowe, Howard Cohen, Sarah Felton, Joseph Spitzer, Ken Raj

^*Corresponding author for this work

UK Health Security Agency

Research output: Contribution to journal › Article › peer-review

Abstract

We have developed a technique to isolate primary keratinocytes, melanocytes, fibroblasts, preadipocytes, and microvascular endothelial cells from an individual sample of human skin. The protocol describes step-by-step instructions for processing, cells isolation, and culture of neonatal foreskin, with adaptation for more demanding adult tissues. The availability of multiple isogenic cell types derived from individual skin samples offers the ability to investigate various areas of biology, in the context of cell-type specificity without potential confounding influence of inter-individual or genetic differences. For complete details on the use and execution of this protocol, please refer to Holliman et al. (2017), Horvath et al. (2019), Horvath et al. (2018), Kabacik et al. (2018), Lowe et al. (2020), Lu et al. (2019), and Lu et al. (2018).

Original language	English
Article number	101378
Journal	STAR Protocols
Volume	3
Issue number	2
DOIs	https://doi.org/10.1016/j.xpro.2022.101378
Publication status	Published - 17 Jun 2022

Bibliographical note

Funding Information:
This study was partly funded by the National Institute for Health Research (NIHR) Health Protection Research Unit in Chemical and Radiation Threats and Hazards, a partnership between Public Health England and Imperial College London. The views expressed are those of the author(s) and not necessarily those of the NIHR, Public Health England, or the Department of Health and Social Care.

Funding Information:
This study was partly funded by the National Institute for Health Research (NIHR) Health Protection Research Unit in Chemical and Radiation Threats and Hazards, a partnership between Public Health England and Imperial College London. The views expressed are those of the author(s) and not necessarily those of the NIHR, Public Health England, or the Department of Health and Social Care. S.K. developed and optimized the protocol, adapted it to the adult tissues, and wrote the manuscript; D.L. developed and optimized the protocol and edited the manuscript; H.C. S.F. and J.S. provided skin tissue samples; K.R. initiated protocol development, acquired funding, and edited the manuscript. The authors declare no competing interests.

Publisher Copyright:
© 2022 The Authors

Keywords

Cell Biology
Cell culture
Cell isolation
Clinical Protocol
Health Sciences

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10.1016/j.xpro.2022.101378

Cite this

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title = "Isolation of five different primary cell types from a single sample of human skin",

abstract = "We have developed a technique to isolate primary keratinocytes, melanocytes, fibroblasts, preadipocytes, and microvascular endothelial cells from an individual sample of human skin. The protocol describes step-by-step instructions for processing, cells isolation, and culture of neonatal foreskin, with adaptation for more demanding adult tissues. The availability of multiple isogenic cell types derived from individual skin samples offers the ability to investigate various areas of biology, in the context of cell-type specificity without potential confounding influence of inter-individual or genetic differences. For complete details on the use and execution of this protocol, please refer to Holliman et al. (2017), Horvath et al. (2019), Horvath et al. (2018), Kabacik et al. (2018), Lowe et al. (2020), Lu et al. (2019), and Lu et al. (2018).",

keywords = "Cell Biology, Cell culture, Cell isolation, Clinical Protocol, Health Sciences",

author = "Sylwia Kabacik and Donna Lowe and Howard Cohen and Sarah Felton and Joseph Spitzer and Ken Raj",

note = "Funding Information: This study was partly funded by the National Institute for Health Research (NIHR) Health Protection Research Unit in Chemical and Radiation Threats and Hazards, a partnership between Public Health England and Imperial College London. The views expressed are those of the author(s) and not necessarily those of the NIHR, Public Health England, or the Department of Health and Social Care. Funding Information: This study was partly funded by the National Institute for Health Research (NIHR) Health Protection Research Unit in Chemical and Radiation Threats and Hazards, a partnership between Public Health England and Imperial College London. The views expressed are those of the author(s) and not necessarily those of the NIHR, Public Health England, or the Department of Health and Social Care. S.K. developed and optimized the protocol, adapted it to the adult tissues, and wrote the manuscript; D.L. developed and optimized the protocol and edited the manuscript; H.C. S.F. and J.S. provided skin tissue samples; K.R. initiated protocol development, acquired funding, and edited the manuscript. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2022 The Authors",

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AU - Raj, Ken

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N2 - We have developed a technique to isolate primary keratinocytes, melanocytes, fibroblasts, preadipocytes, and microvascular endothelial cells from an individual sample of human skin. The protocol describes step-by-step instructions for processing, cells isolation, and culture of neonatal foreskin, with adaptation for more demanding adult tissues. The availability of multiple isogenic cell types derived from individual skin samples offers the ability to investigate various areas of biology, in the context of cell-type specificity without potential confounding influence of inter-individual or genetic differences. For complete details on the use and execution of this protocol, please refer to Holliman et al. (2017), Horvath et al. (2019), Horvath et al. (2018), Kabacik et al. (2018), Lowe et al. (2020), Lu et al. (2019), and Lu et al. (2018).

AB - We have developed a technique to isolate primary keratinocytes, melanocytes, fibroblasts, preadipocytes, and microvascular endothelial cells from an individual sample of human skin. The protocol describes step-by-step instructions for processing, cells isolation, and culture of neonatal foreskin, with adaptation for more demanding adult tissues. The availability of multiple isogenic cell types derived from individual skin samples offers the ability to investigate various areas of biology, in the context of cell-type specificity without potential confounding influence of inter-individual or genetic differences. For complete details on the use and execution of this protocol, please refer to Holliman et al. (2017), Horvath et al. (2019), Horvath et al. (2018), Kabacik et al. (2018), Lowe et al. (2020), Lu et al. (2019), and Lu et al. (2018).

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Isolation of five different primary cell types from a single sample of human skin

Abstract

Bibliographical note

Keywords

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