Isolation and identification of Clostridium spp. from infections associated with the injection of drugs: Experiences of a microbiological investigation team

J. S. Brazier*, B. I. Duerden, V. Hall, J. E. Salmon, J. Hood, M. M. Brett, James McLauchlin, Robert George

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    40 Citations (Scopus)

    Abstract

    Pathogenic species of the genus Clostridium may contaminate the materials used in the injection of drugs and under the right conditions may cause serious or life-threatening disease. C. novyi type A was implicated in an outbreak of severe infection with high mortality in injecting drug users who injected heroin extravascularly. The isolation of such highly oxygen-sensitive clostridia from clinical material may require adherence to enhanced methods and, once isolated, commercially available anaerobe identification kits alone may not give an accurate identification. Additional phenotypic tests that are useful in recognising the main pathogenic species are described. Differentiation of C. novyi type A from C. botulinum type C in reference laboratories was based on 16S rDNA sequence data and specific neutralisation of cytopathic effects in tissue culture.

    Original languageEnglish
    Pages (from-to)985-989
    Number of pages5
    JournalJournal of Medical Microbiology
    Volume51
    Issue number11
    DOIs
    Publication statusPublished - 1 Nov 2002

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