Investigation of transcriptional responses of juvenile mouse bone marrow to power frequency magnetic fields

Sylwia Kabacik; Heide Kirschenlohr; Claudine Raffy; Kevin Whitehill; Margaret Coster; Masumi Abe; Kevin Brindle; Christophe Badie; Zenon Sienkiewicz; Simon Bouffler

doi:10.1016/j.mrfmmm.2013.03.005

Investigation of transcriptional responses of juvenile mouse bone marrow to power frequency magnetic fields

Sylwia Kabacik, Heide Kirschenlohr, Claudine Raffy, Kevin Whitehill, Margaret Coster, Masumi Abe, Kevin Brindle, Christophe Badie, Zenon Sienkiewicz, Simon Bouffler^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

To seek alterations in gene transcription in bone marrow cells following in vivo exposure of juvenile mice to power frequency magnetic fields, young (21-24-day old) C57BL/6 mice were exposed to a 100. μT 50. Hz magnetic field for 2. h. Transcription was analysed by three methods, High Coverage Expression Profiling (HiCEP), Illumina microarrays and quantitative real-time polymerase chain reaction (QRT-PCR). A pilot HiCEP experiment with 6 exposed (E) and 6 non-exposed (NE) mice identified four candidate responsive transcripts (two unknown transcripts (AK152075 and F10-NED), phosphatidylinositol binding clathrin assembly protein (Picalm) and exportin 7 (Xpo7)). A larger experiment compared 19 E and 15 NE mice using two independent QRT-PCR assays and repeated microarray assays. No significant field-dependent changes were seen, although Picalm showed a trend to significance in one QRT-PCR assay (E/NE = 0.91; P=0.06). However, the study was underpowered to detect an effect of this magnitude (52% power at P=0.05). These data indicate the current experimental constraints in detecting small changes in transcription that may occur in response to magnetic fields. These constraints result from technical limitations in the accuracy of assays and biological variation, which together were sufficient to account statistically for the number of differentially expressed transcripts identified in the pilot experiment.

Original language	English
Pages (from-to)	40-45
Number of pages	6
Journal	Mutation Research
Volume	745-746
DOIs	https://doi.org/10.1016/j.mrfmmm.2013.03.005
Publication status	Published - May 2013

Bibliographical note

Funding Information:
We thank Prof. JC Metcalfe and Dr TR Hesketh for helpful discussions throughout the project. This project was funded in part by a grant to ZS, CB, SB and KB from the EMF Biological Research Trust (registered charity no. 1042132) and the National Institute for Health Research Centre for Public Health Protection Research at the Health Protection Agency.

Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.

Keywords

Bone marrow
Juvenile
Magnetic fields
Mouse
Transcription

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10.1016/j.mrfmmm.2013.03.005

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title = "Investigation of transcriptional responses of juvenile mouse bone marrow to power frequency magnetic fields",

abstract = "To seek alterations in gene transcription in bone marrow cells following in vivo exposure of juvenile mice to power frequency magnetic fields, young (21-24-day old) C57BL/6 mice were exposed to a 100. μT 50. Hz magnetic field for 2. h. Transcription was analysed by three methods, High Coverage Expression Profiling (HiCEP), Illumina microarrays and quantitative real-time polymerase chain reaction (QRT-PCR). A pilot HiCEP experiment with 6 exposed (E) and 6 non-exposed (NE) mice identified four candidate responsive transcripts (two unknown transcripts (AK152075 and F10-NED), phosphatidylinositol binding clathrin assembly protein (Picalm) and exportin 7 (Xpo7)). A larger experiment compared 19 E and 15 NE mice using two independent QRT-PCR assays and repeated microarray assays. No significant field-dependent changes were seen, although Picalm showed a trend to significance in one QRT-PCR assay (E/NE = 0.91; P=0.06). However, the study was underpowered to detect an effect of this magnitude (52% power at P=0.05). These data indicate the current experimental constraints in detecting small changes in transcription that may occur in response to magnetic fields. These constraints result from technical limitations in the accuracy of assays and biological variation, which together were sufficient to account statistically for the number of differentially expressed transcripts identified in the pilot experiment.",

keywords = "Bone marrow, Juvenile, Magnetic fields, Mouse, Transcription",

author = "Sylwia Kabacik and Heide Kirschenlohr and Claudine Raffy and Kevin Whitehill and Margaret Coster and Masumi Abe and Kevin Brindle and Christophe Badie and Zenon Sienkiewicz and Simon Bouffler",

note = "Funding Information: We thank Prof. JC Metcalfe and Dr TR Hesketh for helpful discussions throughout the project. This project was funded in part by a grant to ZS, CB, SB and KB from the EMF Biological Research Trust (registered charity no. 1042132) and the National Institute for Health Research Centre for Public Health Protection Research at the Health Protection Agency. Copyright: Copyright 2013 Elsevier B.V., All rights reserved.",

year = "2013",

month = may,

doi = "10.1016/j.mrfmmm.2013.03.005",

language = "English",

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journal = "Mutation Research",

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AU - Kabacik, Sylwia

AU - Kirschenlohr, Heide

AU - Raffy, Claudine

AU - Whitehill, Kevin

AU - Coster, Margaret

AU - Abe, Masumi

AU - Brindle, Kevin

AU - Badie, Christophe

AU - Sienkiewicz, Zenon

AU - Bouffler, Simon

N1 - Funding Information: We thank Prof. JC Metcalfe and Dr TR Hesketh for helpful discussions throughout the project. This project was funded in part by a grant to ZS, CB, SB and KB from the EMF Biological Research Trust (registered charity no. 1042132) and the National Institute for Health Research Centre for Public Health Protection Research at the Health Protection Agency. Copyright: Copyright 2013 Elsevier B.V., All rights reserved.

PY - 2013/5

Y1 - 2013/5

N2 - To seek alterations in gene transcription in bone marrow cells following in vivo exposure of juvenile mice to power frequency magnetic fields, young (21-24-day old) C57BL/6 mice were exposed to a 100. μT 50. Hz magnetic field for 2. h. Transcription was analysed by three methods, High Coverage Expression Profiling (HiCEP), Illumina microarrays and quantitative real-time polymerase chain reaction (QRT-PCR). A pilot HiCEP experiment with 6 exposed (E) and 6 non-exposed (NE) mice identified four candidate responsive transcripts (two unknown transcripts (AK152075 and F10-NED), phosphatidylinositol binding clathrin assembly protein (Picalm) and exportin 7 (Xpo7)). A larger experiment compared 19 E and 15 NE mice using two independent QRT-PCR assays and repeated microarray assays. No significant field-dependent changes were seen, although Picalm showed a trend to significance in one QRT-PCR assay (E/NE = 0.91; P=0.06). However, the study was underpowered to detect an effect of this magnitude (52% power at P=0.05). These data indicate the current experimental constraints in detecting small changes in transcription that may occur in response to magnetic fields. These constraints result from technical limitations in the accuracy of assays and biological variation, which together were sufficient to account statistically for the number of differentially expressed transcripts identified in the pilot experiment.

AB - To seek alterations in gene transcription in bone marrow cells following in vivo exposure of juvenile mice to power frequency magnetic fields, young (21-24-day old) C57BL/6 mice were exposed to a 100. μT 50. Hz magnetic field for 2. h. Transcription was analysed by three methods, High Coverage Expression Profiling (HiCEP), Illumina microarrays and quantitative real-time polymerase chain reaction (QRT-PCR). A pilot HiCEP experiment with 6 exposed (E) and 6 non-exposed (NE) mice identified four candidate responsive transcripts (two unknown transcripts (AK152075 and F10-NED), phosphatidylinositol binding clathrin assembly protein (Picalm) and exportin 7 (Xpo7)). A larger experiment compared 19 E and 15 NE mice using two independent QRT-PCR assays and repeated microarray assays. No significant field-dependent changes were seen, although Picalm showed a trend to significance in one QRT-PCR assay (E/NE = 0.91; P=0.06). However, the study was underpowered to detect an effect of this magnitude (52% power at P=0.05). These data indicate the current experimental constraints in detecting small changes in transcription that may occur in response to magnetic fields. These constraints result from technical limitations in the accuracy of assays and biological variation, which together were sufficient to account statistically for the number of differentially expressed transcripts identified in the pilot experiment.

KW - Bone marrow

KW - Juvenile

KW - Magnetic fields

KW - Mouse

KW - Transcription

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DO - 10.1016/j.mrfmmm.2013.03.005

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AN - SCOPUS:84878253645

SN - 0027-5107

VL - 745-746

SP - 40

EP - 45

JO - Mutation Research

JF - Mutation Research

ER -

Investigation of transcriptional responses of juvenile mouse bone marrow to power frequency magnetic fields

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Keywords

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