Introduction of PCR testing reveals a previously unrecognized burden of yersiniosis in Hampshire, UK

Mattea Clarke, Girija Dabke, Lenka Strakova, Claire Jenkins, Maria Saavedra-Campos, Oliver McManus, Karthik Paranthaman

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Introduction: Current testing practices for yersiniosis mean that its true incidence and epidemiology are not well understood. In mid-2016, the introduction of testing via a multiplex gastrointestinal PCR panel at Portsmouth hospital laboratory in Hampshire, UK, resulted in a marked increase in the number of Yersinia cases identified locally.

Aim: Here we describe the epidemiology and microbiology of Yersinia cases identified at Portsmouth laboratory following the introduction of PCR testing. Methodology: A case was defined as a person with a stool specimen in which Yersinia was detected by PCR and/or culture at Portsmouth NHS Trust laboratory between 1 January 2014 and 31 December 2018. A case list was created from laboratory data submitted by Portsmouth laboratory to Public Health England (PHE), updated with speciation and serotyping data from the PHE reference laboratory. Descriptive analysis was performed. Results: Over 30 months following introduction of PCR testing, 199 cases were confirmed with Yersinia, compared to two cases in the preceding 30 months. This corresponds to a rate of 13.8 and 0.1 per 100 000 population per year respectively (P<0.0001). In total, 85% of tested isolates were Y. enterocolitica, belonging to multiple serotypes, and the rest belonged to a range of Y. enterocolitica-like species.

Conclusions: Introduction of PCR testing led to the identification of a previously unrecognized burden of yersiniosis in Hampshire. The diversity of species and serotypes suggests heterogeneity in sources and transmission routes. Further research on exposures, risk factors and clinical sequalae is needed to improve our understanding of the clinical and public health impact.

Original languageEnglish
Pages (from-to)419-426
Number of pages8
JournalJournal of Medical Microbiology
Volume69
Issue number3
DOIs
Publication statusPublished - 30 Jan 2020

Bibliographical note

Funding information: This work received no specific grant from any funding agency

Open Access: Free to read, but no Open Access licence

Publisher Copyright: © 2020 Crown Copyright

Citation: Clarke, Mattea, et al. "Introduction of PCR testing reveals a previously unrecognized burden of yersiniosis in Hampshire, UK." Journal of medical microbiology 69.3 (2020): 419-426.

DOI: https://doi.org/10.1099/jmm.0.001125

Keywords

  • Epidemiology
  • Gastroenteritis
  • Yersinia

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