International study of factors affecting human chromosome translocations

Alice J. Sigurdson; Mina Ha; Michael Hauptmann; Parveen Bhatti; Radim J. Sram; Olena Beskid; E. Janet Tawn; Caroline A. Whitehouse; Carita Lindholm; Mimako Nakano; Yoshiaki Kodama; Nori Nakamura; Irena Vorobtsova; Ursula Oestreicher; Günther Stephan; Lee C. Yong; Manfred Bauchinger; Ernst Schmid; Hai Won Chung; Firouz Darroudi; Laurence Roy; Phillipe Voisin; Joan F. Barquinero; Gordon Livingston; David Blakey; Isamu Hayata; Wei Zhang; Chunyan Wang; L. Michelle Bennett; L. Gayle Littlefield; Alan A. Edwards; Ruth A. Kleinerman; James D. Tucker

doi:10.1016/j.mrgentox.2008.01.005

International study of factors affecting human chromosome translocations

Alice J. Sigurdson
, Mina Ha
, Michael Hauptmann
, Parveen Bhatti
, Radim J. Sram
, Olena Beskid
, E. Janet Tawn
, Caroline A. Whitehouse
, Carita Lindholm
, Mimako Nakano
, Yoshiaki Kodama
, Nori Nakamura
, Irena Vorobtsova
, Ursula Oestreicher
, Günther Stephan
, Lee C. Yong
, Manfred Bauchinger
, Ernst Schmid
, Hai Won Chung
, Firouz Darroudi

Laurence Roy, Phillipe Voisin, Joan F. Barquinero, Gordon Livingston, David Blakey, Isamu Hayata, Wei Zhang, Chunyan Wang, L. Michelle Bennett, L. Gayle Littlefield, Alan A. Edwards, Ruth A. Kleinerman, James D. Tucker^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

134 Citations (Scopus)

Abstract

Chromosome translocations in peripheral blood lymphocytes of normal, healthy humans increase with age, but the effects of gender, race, and cigarette smoking on background translocation yields have not been examined systematically. Further, the shape of the relationship between age and translocation frequency (TF) has not been definitively determined. We collected existing data from 16 laboratories in North America, Europe, and Asia on TFs measured in peripheral blood lymphocytes by fluorescence in situ hybridization whole chromosome painting among 1933 individuals. In Poisson regression models, age, ranging from newborns (cord blood) to 85 years, was strongly associated with TF and this relationship showed significant upward curvature at older ages versus a linear relationship (p < 0.001). Ever smokers had significantly higher TFs than non-smokers (rate ratio (RR) = 1.19, 95% confidence interval (CI), 1.09-1.30) and smoking modified the effect of age on TFs with a steeper age-related increase among ever smokers compared to non-smokers (p < 0.001). TFs did not differ by gender. Interpreting an independent effect of race was difficult owing to laboratory variation. Our study is three times larger than any pooled effort to date, confirming a suspected curvilinear relationship of TF with age. The significant effect of cigarette smoking has not been observed with previous pooled studies of TF in humans. Our data provide stable estimates of background TF by age, gender, race, and smoking status and suggest an acceleration of chromosome damage above age 60 and among those with a history of smoking cigarettes.

Original language	English
Pages (from-to)	112-121
Number of pages	10
Journal	Mutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume	652
Issue number	2
DOIs	https://doi.org/10.1016/j.mrgentox.2008.01.005
Publication status	Published - 30 Apr 2008

Bibliographical note

Funding Information:
This research was supported in part by the Intramural Research Program of the Division of Cancer Epidemiology and Genetics, the National Cancer Institute (NCI), NIH, DHHS and by an Intra-agency agreement between the National Institute of Allergy and Infectious Diseases (NIAID) and the NCI, NIAID agreement #Y2-A1-5077 and #Y3-CO-5117; performed in part under the auspices of the US Department of Energy by the Lawrence Livermore National Laboratory from contract No. W-7405-ENG-48; CEC Concerted Action (FIGD-CT-200-20040; a grant from the Czech Ministry of Environment VaV/340/2/00, VaV/740/5/03 and VaV-SL/5/160/05; The Radiation Effects Research Foundation (RERF), Hiroshima and Nagasaki, Japan, is a private, non-profit foundation funded by the Japanese Ministry of Health, Labour and Welfare (MHLW) and the US Department of Energy (DOE), the latter through the National Academy of Sciences, the present publication was supported by RERF Research Protocol RP 8-93; The Spanish Nuclear Safety Council (ref 246/96); The European Union SOUL project (FIP6R-516478); a grant from the Russian Foundation for Basic Research, 01-0449118; British Nuclear Fuels plc, United Kingdom; an interagency agreement between the NCI and the National Institute for Occupational Safety and Health, Y1-CP-9012. The findings and conclusions in this report are those of the author(s) and do not necessarily represent the views of the National Institute for Occupational Safety and Health.

Keywords

Background frequency
Chromosome translocations
Controls
Fluorescence in situ hybridization

Access to Document

10.1016/j.mrgentox.2008.01.005

Cite this

Sigurdson, A. J., Ha, M., Hauptmann, M., Bhatti, P., Sram, R. J., Beskid, O., Tawn, E. J., Whitehouse, C. A., Lindholm, C., Nakano, M., Kodama, Y., Nakamura, N., Vorobtsova, I., Oestreicher, U., Stephan, G., Yong, L. C., Bauchinger, M., Schmid, E., Chung, H. W., ... Tucker, J. D. (2008). International study of factors affecting human chromosome translocations. Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 652(2), 112-121. https://doi.org/10.1016/j.mrgentox.2008.01.005

@article{549db126fadb4fa7b690677863a480a0,

title = "International study of factors affecting human chromosome translocations",

abstract = "Chromosome translocations in peripheral blood lymphocytes of normal, healthy humans increase with age, but the effects of gender, race, and cigarette smoking on background translocation yields have not been examined systematically. Further, the shape of the relationship between age and translocation frequency (TF) has not been definitively determined. We collected existing data from 16 laboratories in North America, Europe, and Asia on TFs measured in peripheral blood lymphocytes by fluorescence in situ hybridization whole chromosome painting among 1933 individuals. In Poisson regression models, age, ranging from newborns (cord blood) to 85 years, was strongly associated with TF and this relationship showed significant upward curvature at older ages versus a linear relationship (p < 0.001). Ever smokers had significantly higher TFs than non-smokers (rate ratio (RR) = 1.19, 95\% confidence interval (CI), 1.09-1.30) and smoking modified the effect of age on TFs with a steeper age-related increase among ever smokers compared to non-smokers (p < 0.001). TFs did not differ by gender. Interpreting an independent effect of race was difficult owing to laboratory variation. Our study is three times larger than any pooled effort to date, confirming a suspected curvilinear relationship of TF with age. The significant effect of cigarette smoking has not been observed with previous pooled studies of TF in humans. Our data provide stable estimates of background TF by age, gender, race, and smoking status and suggest an acceleration of chromosome damage above age 60 and among those with a history of smoking cigarettes.",

keywords = "Background frequency, Chromosome translocations, Controls, Fluorescence in situ hybridization",

author = "Sigurdson, \{Alice J.\} and Mina Ha and Michael Hauptmann and Parveen Bhatti and Sram, \{Radim J.\} and Olena Beskid and Tawn, \{E. Janet\} and Whitehouse, \{Caroline A.\} and Carita Lindholm and Mimako Nakano and Yoshiaki Kodama and Nori Nakamura and Irena Vorobtsova and Ursula Oestreicher and G{\"u}nther Stephan and Yong, \{Lee C.\} and Manfred Bauchinger and Ernst Schmid and Chung, \{Hai Won\} and Firouz Darroudi and Laurence Roy and Phillipe Voisin and Barquinero, \{Joan F.\} and Gordon Livingston and David Blakey and Isamu Hayata and Wei Zhang and Chunyan Wang and Bennett, \{L. Michelle\} and Littlefield, \{L. Gayle\} and Edwards, \{Alan A.\} and Kleinerman, \{Ruth A.\} and Tucker, \{James D.\}",

note = "Funding Information: This research was supported in part by the Intramural Research Program of the Division of Cancer Epidemiology and Genetics, the National Cancer Institute (NCI), NIH, DHHS and by an Intra-agency agreement between the National Institute of Allergy and Infectious Diseases (NIAID) and the NCI, NIAID agreement \#Y2-A1-5077 and \#Y3-CO-5117; performed in part under the auspices of the US Department of Energy by the Lawrence Livermore National Laboratory from contract No. W-7405-ENG-48; CEC Concerted Action (FIGD-CT-200-20040; a grant from the Czech Ministry of Environment VaV/340/2/00, VaV/740/5/03 and VaV-SL/5/160/05; The Radiation Effects Research Foundation (RERF), Hiroshima and Nagasaki, Japan, is a private, non-profit foundation funded by the Japanese Ministry of Health, Labour and Welfare (MHLW) and the US Department of Energy (DOE), the latter through the National Academy of Sciences, the present publication was supported by RERF Research Protocol RP 8-93; The Spanish Nuclear Safety Council (ref 246/96); The European Union SOUL project (FIP6R-516478); a grant from the Russian Foundation for Basic Research, 01-0449118; British Nuclear Fuels plc, United Kingdom; an interagency agreement between the NCI and the National Institute for Occupational Safety and Health, Y1-CP-9012. The findings and conclusions in this report are those of the author(s) and do not necessarily represent the views of the National Institute for Occupational Safety and Health. ",

year = "2008",

month = apr,

day = "30",

doi = "10.1016/j.mrgentox.2008.01.005",

language = "English",

volume = "652",

pages = "112--121",

journal = "Mutation Research - Genetic Toxicology and Environmental Mutagenesis",

issn = "1383-5718",

publisher = "Elsevier B.V.",

number = "2",

}

Sigurdson, AJ, Ha, M, Hauptmann, M, Bhatti, P, Sram, RJ, Beskid, O, Tawn, EJ, Whitehouse, CA, Lindholm, C, Nakano, M, Kodama, Y, Nakamura, N, Vorobtsova, I, Oestreicher, U, Stephan, G, Yong, LC, Bauchinger, M, Schmid, E, Chung, HW, Darroudi, F, Roy, L, Voisin, P, Barquinero, JF, Livingston, G, Blakey, D, Hayata, I, Zhang, W, Wang, C, Bennett, LM, Littlefield, LG, Edwards, AA, Kleinerman, RA & Tucker, JD 2008, 'International study of factors affecting human chromosome translocations', Mutation Research - Genetic Toxicology and Environmental Mutagenesis, vol. 652, no. 2, pp. 112-121. https://doi.org/10.1016/j.mrgentox.2008.01.005

TY - JOUR

T1 - International study of factors affecting human chromosome translocations

AU - Sigurdson, Alice J.

AU - Ha, Mina

AU - Hauptmann, Michael

AU - Bhatti, Parveen

AU - Sram, Radim J.

AU - Beskid, Olena

AU - Tawn, E. Janet

AU - Whitehouse, Caroline A.

AU - Lindholm, Carita

AU - Nakano, Mimako

AU - Kodama, Yoshiaki

AU - Nakamura, Nori

AU - Vorobtsova, Irena

AU - Oestreicher, Ursula

AU - Stephan, Günther

AU - Yong, Lee C.

AU - Bauchinger, Manfred

AU - Schmid, Ernst

AU - Chung, Hai Won

AU - Darroudi, Firouz

AU - Roy, Laurence

AU - Voisin, Phillipe

AU - Barquinero, Joan F.

AU - Livingston, Gordon

AU - Blakey, David

AU - Hayata, Isamu

AU - Zhang, Wei

AU - Wang, Chunyan

AU - Bennett, L. Michelle

AU - Littlefield, L. Gayle

AU - Edwards, Alan A.

AU - Kleinerman, Ruth A.

AU - Tucker, James D.

N1 - Funding Information: This research was supported in part by the Intramural Research Program of the Division of Cancer Epidemiology and Genetics, the National Cancer Institute (NCI), NIH, DHHS and by an Intra-agency agreement between the National Institute of Allergy and Infectious Diseases (NIAID) and the NCI, NIAID agreement #Y2-A1-5077 and #Y3-CO-5117; performed in part under the auspices of the US Department of Energy by the Lawrence Livermore National Laboratory from contract No. W-7405-ENG-48; CEC Concerted Action (FIGD-CT-200-20040; a grant from the Czech Ministry of Environment VaV/340/2/00, VaV/740/5/03 and VaV-SL/5/160/05; The Radiation Effects Research Foundation (RERF), Hiroshima and Nagasaki, Japan, is a private, non-profit foundation funded by the Japanese Ministry of Health, Labour and Welfare (MHLW) and the US Department of Energy (DOE), the latter through the National Academy of Sciences, the present publication was supported by RERF Research Protocol RP 8-93; The Spanish Nuclear Safety Council (ref 246/96); The European Union SOUL project (FIP6R-516478); a grant from the Russian Foundation for Basic Research, 01-0449118; British Nuclear Fuels plc, United Kingdom; an interagency agreement between the NCI and the National Institute for Occupational Safety and Health, Y1-CP-9012. The findings and conclusions in this report are those of the author(s) and do not necessarily represent the views of the National Institute for Occupational Safety and Health.

PY - 2008/4/30

Y1 - 2008/4/30

N2 - Chromosome translocations in peripheral blood lymphocytes of normal, healthy humans increase with age, but the effects of gender, race, and cigarette smoking on background translocation yields have not been examined systematically. Further, the shape of the relationship between age and translocation frequency (TF) has not been definitively determined. We collected existing data from 16 laboratories in North America, Europe, and Asia on TFs measured in peripheral blood lymphocytes by fluorescence in situ hybridization whole chromosome painting among 1933 individuals. In Poisson regression models, age, ranging from newborns (cord blood) to 85 years, was strongly associated with TF and this relationship showed significant upward curvature at older ages versus a linear relationship (p < 0.001). Ever smokers had significantly higher TFs than non-smokers (rate ratio (RR) = 1.19, 95% confidence interval (CI), 1.09-1.30) and smoking modified the effect of age on TFs with a steeper age-related increase among ever smokers compared to non-smokers (p < 0.001). TFs did not differ by gender. Interpreting an independent effect of race was difficult owing to laboratory variation. Our study is three times larger than any pooled effort to date, confirming a suspected curvilinear relationship of TF with age. The significant effect of cigarette smoking has not been observed with previous pooled studies of TF in humans. Our data provide stable estimates of background TF by age, gender, race, and smoking status and suggest an acceleration of chromosome damage above age 60 and among those with a history of smoking cigarettes.

AB - Chromosome translocations in peripheral blood lymphocytes of normal, healthy humans increase with age, but the effects of gender, race, and cigarette smoking on background translocation yields have not been examined systematically. Further, the shape of the relationship between age and translocation frequency (TF) has not been definitively determined. We collected existing data from 16 laboratories in North America, Europe, and Asia on TFs measured in peripheral blood lymphocytes by fluorescence in situ hybridization whole chromosome painting among 1933 individuals. In Poisson regression models, age, ranging from newborns (cord blood) to 85 years, was strongly associated with TF and this relationship showed significant upward curvature at older ages versus a linear relationship (p < 0.001). Ever smokers had significantly higher TFs than non-smokers (rate ratio (RR) = 1.19, 95% confidence interval (CI), 1.09-1.30) and smoking modified the effect of age on TFs with a steeper age-related increase among ever smokers compared to non-smokers (p < 0.001). TFs did not differ by gender. Interpreting an independent effect of race was difficult owing to laboratory variation. Our study is three times larger than any pooled effort to date, confirming a suspected curvilinear relationship of TF with age. The significant effect of cigarette smoking has not been observed with previous pooled studies of TF in humans. Our data provide stable estimates of background TF by age, gender, race, and smoking status and suggest an acceleration of chromosome damage above age 60 and among those with a history of smoking cigarettes.

KW - Background frequency

KW - Chromosome translocations

KW - Controls

KW - Fluorescence in situ hybridization

UR - https://www.scopus.com/pages/publications/43049090758

U2 - 10.1016/j.mrgentox.2008.01.005

DO - 10.1016/j.mrgentox.2008.01.005

M3 - Article

C2 - 18337160

AN - SCOPUS:43049090758

SN - 1383-5718

VL - 652

SP - 112

EP - 121

JO - Mutation Research - Genetic Toxicology and Environmental Mutagenesis

JF - Mutation Research - Genetic Toxicology and Environmental Mutagenesis

IS - 2

ER -

International study of factors affecting human chromosome translocations

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this