TY - JOUR
T1 - International laboratory comparison of influenza microneutralization assays for A(H1N1)pdm09, A(H3N2), and A(H5N1) influenza viruses by CONSISE
AU - the Consise Laboratory Working Group Participants
AU - Laurie, Karen L.
AU - Engelhardt, Othmar G.
AU - Wood, John
AU - Heath, Alan
AU - Katz, Jacqueline M.
AU - Peiris, Malik
AU - Hoschler, Katja
AU - Hungnes, Olav
AU - Zhang, Wenqing
AU - Van Kerkhove, Maria D.
AU - Katz, Jaccqueline
AU - Lu, Xiuhua
AU - Levine, Min
AU - Veguilla, Vic
AU - Liu, Feng
AU - Bai, Yaohui
AU - Puthavathana, Pilaipan
AU - Lerdsamran, Hatairat
AU - Pooruk, Phisanu
AU - Nateerom, Knnika
AU - Castrucci, Maria Rita
AU - Donatelli, Isabella
AU - Facchini, Marzia
AU - Kishida, Noriko
AU - Tashiro, Masato
AU - Odagiri, Takato
AU - Pawar, Shailesh D.
AU - Kode, Sadhana S.
AU - Hawksworth, Anthony
AU - Ortiguerra, Ryan
AU - Brice, Gary
AU - Martin, Nicholas
AU - Kochel, Tad
AU - Sanchez, Jose
AU - Cooper, Michael
AU - Cummings, James
AU - Wagner, Ralf
AU - Goepfert, Constanze
AU - Alex, Nina
AU - Hammann, Joanna
AU - Neumann, Britta
AU - Perera, Mahendra
AU - Montomoli, Emanuele
AU - Lapini, Guilia
AU - Sbragi, Sara
AU - Bai, Tian
AU - Yu, Zaijiang
AU - Zhou, Jianfang
AU - Carolan, Louise
AU - Laurie, Karen
N1 - Publisher Copyright:
Copyright © 2015, American Society for Microbiology. All Rights Reserved.
PY - 2015/8/1
Y1 - 2015/8/1
N2 - The microneutralization assay is commonly used to detect antibodies to influenza virus, and multiple protocols are used worldwide. These protocols differ in the incubation time of the assay as well as in the order of specific steps, and even within protocols there are often further adjustments in individual laboratories. The impact these protocol variations have on influenza serology data is unclear. Thus, a laboratory comparison of the 2-day enzyme-linked immunosorbent assay (ELISA) and 3-day hemagglutination (HA) microneutralization (MN) protocols, using A(H1N1)pdm09, A(H3N2), and A(H5N1) viruses, was performed by the CONSISE Laboratory Working Group. Individual laboratories performed both assay protocols, on multiple occasions, using different serum panels. Thirteen laboratories from around the world participated. Within each laboratory, serum sample titers for the different assay protocols were compared between assays to determine the sensitivity of each assay and were compared between replicates to assess the reproducibility of each protocol for each laboratory. There was good correlation of the results obtained using the two assay protocols in most laboratories, indicating that these assays may be interchangeable for detecting antibodies to the influenza A viruses included in this study. Importantly, participating laboratories have aligned their methodologies to the CONSISE consensus 2-day ELISA and 3-day HAMNassay protocols to enable better correlation of these assays in the future.
AB - The microneutralization assay is commonly used to detect antibodies to influenza virus, and multiple protocols are used worldwide. These protocols differ in the incubation time of the assay as well as in the order of specific steps, and even within protocols there are often further adjustments in individual laboratories. The impact these protocol variations have on influenza serology data is unclear. Thus, a laboratory comparison of the 2-day enzyme-linked immunosorbent assay (ELISA) and 3-day hemagglutination (HA) microneutralization (MN) protocols, using A(H1N1)pdm09, A(H3N2), and A(H5N1) viruses, was performed by the CONSISE Laboratory Working Group. Individual laboratories performed both assay protocols, on multiple occasions, using different serum panels. Thirteen laboratories from around the world participated. Within each laboratory, serum sample titers for the different assay protocols were compared between assays to determine the sensitivity of each assay and were compared between replicates to assess the reproducibility of each protocol for each laboratory. There was good correlation of the results obtained using the two assay protocols in most laboratories, indicating that these assays may be interchangeable for detecting antibodies to the influenza A viruses included in this study. Importantly, participating laboratories have aligned their methodologies to the CONSISE consensus 2-day ELISA and 3-day HAMNassay protocols to enable better correlation of these assays in the future.
UR - http://www.scopus.com/inward/record.url?scp=84939480223&partnerID=8YFLogxK
U2 - 10.1128/CVI.00278-15
DO - 10.1128/CVI.00278-15
M3 - Article
C2 - 26108286
AN - SCOPUS:84939480223
SN - 1556-6811
VL - 22
SP - 957
EP - 964
JO - Clinical and Vaccine Immunology
JF - Clinical and Vaccine Immunology
IS - 8
ER -