TY - JOUR
T1 - Interlaboratory comparison of PCR-based identification and genogrouping of Neisseria meningitidis
AU - Taha, Muhamed Kheir
AU - Alonso, Jean Michel
AU - Cafferkey, Mary
AU - Caugant, Dominique A.
AU - Clarke, Stuart C.
AU - Diggle, Mathew A.
AU - Fox, Andrew
AU - Frosch, Matthias
AU - Gray, Stephen J.
AU - Guiver, Malcolm
AU - Heuberger, Sigrid
AU - Kalmusova, Jitka
AU - Kesanopoulos, Konstantinos
AU - Klem, Anne Marie
AU - Kriz, Paula
AU - Marsh, John
AU - Mölling, Paula
AU - Murphy, Karen
AU - Olcén, Per
AU - Sanou, Oumar
AU - Tzanakaki, Georgina
AU - Vogel, Ulrich
PY - 2005/1
Y1 - 2005/1
N2 - Twenty clinical samples (18 cerebrospinal fluid samples and 2 articular fluid samples) were sent to 11 meningococcus reference centers located in 11 different countries. Ten of these laboratories are participating in the EU-MenNet program (a European Union-funded program) and are members of the European Monitoring Group on Meningococci. The remaining laboratory was located in Burkina Faso. Neisseria meningitidis was sought by detecting several meningococcus-specific genes (crgA, ctrA, 16S rRNA, and porA). The PCR-based nonculture method for the detection of N. meningitidis gave similar results between participants with a mean sensitivity and specificity of 89.7 and 92.7%, respectively. Most of the laboratories also performed genogrouping assays (siaD and mynB/sacC). The performance of genogrouping was more variable between laboratories, with a mean sensitivity of 72.7%. Genogroup B gave the best correlation between participants, as all laboratories routinely perform this PCR. The results for genogroups A and W135 were less similar between the eight participating laboratories that performed these PCRs.
AB - Twenty clinical samples (18 cerebrospinal fluid samples and 2 articular fluid samples) were sent to 11 meningococcus reference centers located in 11 different countries. Ten of these laboratories are participating in the EU-MenNet program (a European Union-funded program) and are members of the European Monitoring Group on Meningococci. The remaining laboratory was located in Burkina Faso. Neisseria meningitidis was sought by detecting several meningococcus-specific genes (crgA, ctrA, 16S rRNA, and porA). The PCR-based nonculture method for the detection of N. meningitidis gave similar results between participants with a mean sensitivity and specificity of 89.7 and 92.7%, respectively. Most of the laboratories also performed genogrouping assays (siaD and mynB/sacC). The performance of genogrouping was more variable between laboratories, with a mean sensitivity of 72.7%. Genogroup B gave the best correlation between participants, as all laboratories routinely perform this PCR. The results for genogroups A and W135 were less similar between the eight participating laboratories that performed these PCRs.
UR - https://www.scopus.com/pages/publications/19944428227
U2 - 10.1128/JCM.43.1.144-149.2005
DO - 10.1128/JCM.43.1.144-149.2005
M3 - Article
C2 - 15634963
AN - SCOPUS:19944428227
SN - 0095-1137
VL - 43
SP - 144
EP - 149
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 1
ER -