Influenza Virological Surveillance in Children: The Use of the QuickVue Rapid Diagnostic Test

Fabrizio Pregliasco; Simona Puzelli; Carolina Mensi; Giovanni Anselmi; Roberto Marinello; M. Luisa Tanzi; Chiara Affinito; Maria Zambon; Isabella Donatelli; Francesco Argiro'; Emilio Bergonzi; Valeria Cammareri; Mauro Casati; Antonello Ciarroca; Salvatore Curto; Carlo Davoli; Giuseppe Elio; Concetta Fabiani; Renato Facconi; Giovanni Fasani; Francesca Giussani; Fabiola Frezza; Marco Giussani; Luigi Greco; Carlo Longhi; Nella Mezzabotta; Rinaldo Missaglia; Romualdo Moggio; Marco Montalbetti; Luisa Maria Nino; Alberto Pedone; Marina Picca; Letizia Raimondi; Albino Ronchi; Rino Rosignoli; Luigi Siccardo; Giuseppe Varrasi; Guido Vertua

doi:10.1002/jmv.20086

Influenza Virological Surveillance in Children: The Use of the QuickVue Rapid Diagnostic Test

Fabrizio Pregliasco, Simona Puzelli, Carolina Mensi, Giovanni Anselmi, Roberto Marinello, M. Luisa Tanzi, Chiara Affinito, Maria Zambon, Isabella Donatelli^*, Francesco Argiro', Emilio Bergonzi, Valeria Cammareri, Mauro Casati, Antonello Ciarroca, Salvatore Curto, Carlo Davoli, Giuseppe Elio, Concetta Fabiani, Renato Facconi, Giovanni FasaniFrancesca Giussani, Fabiola Frezza, Marco Giussani, Luigi Greco, Carlo Longhi, Nella Mezzabotta, Rinaldo Missaglia, Romualdo Moggio, Marco Montalbetti, Luisa Maria Nino, Alberto Pedone, Marina Picca, Letizia Raimondi, Albino Ronchi, Rino Rosignoli, Luigi Siccardo, Giuseppe Varrasi, Guido Vertua

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Abstract

New rapid diagnostic methods are needed to identify influenza infections to improve virological surveillance usually undertaken with conventional time-consuming, complex, and even expensive laboratory methods. Another reason for using a rapid test is to avoid inappropriate therapy, particularly in children, where use of antibiotics inappropriately and high influenza-related rates of hospitalisation are described. During two winter seasons, the performance of the QuickVue® Influenza test (QV) was evaluated in children under 14 presenting with influenza like illness, and compared the results with those obtained from sentinel network surveillance using standard protocols for the sample collection and the laboratory analysis by virus culture and reverse transcription-polymerase chain reaction (RT-PCR). During the first influenza season (2000/2001), 22 paediatricians collected one nose- and one throat-swab from each of the 586 children 0-6 years old recruited in the study. The QV test was carried out in the physician's office by primary care staff on the nose swab material. When compared with virus culture of the throat swab, the QV test had a sensitivity of 36.5%. In the following 2001/2002-influenza season, the performance of the QV test as a rapid laboratory screening assay was assessed. 342 children aged 0-14 years were enrolled with only one throat swab collected from each patient and sent to the laboratory where the QV, virus culture, and RT-PCR tests were performed. The results showed a better sensitivity (54.5%) of the test in comparison with virus culture and RT-PCR assays. The data indicate that rapid QV testing in the physician office setting, using these easily obtained samples, may be too insensitive to be useful for surveillance and for immediate clinical management of children presenting with influenza-like illness. Nevertheless, the QV test may be a valuable diagnostic tool if used in laboratory, as a rapid screening test.

Original language	English
Pages (from-to)	269-273
Number of pages	5
Journal	Journal of Medical Virology
Volume	73
Issue number	2
DOIs	https://doi.org/10.1002/jmv.20086
Publication status	Published - Jun 2004

Keywords

Children
Diagnosis
Influenza virus
Polymerase chain reaction
Rapid test
Virus culture

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/jmv.20086

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Pregliasco, F., Puzelli, S., Mensi, C., Anselmi, G., Marinello, R., Luisa Tanzi, M., Affinito, C., Zambon, M., Donatelli, I., Argiro', F., Bergonzi, E., Cammareri, V., Casati, M., Ciarroca, A., Curto, S., Davoli, C., Elio, G., Fabiani, C., Facconi, R., ... Vertua, G. (2004). Influenza Virological Surveillance in Children: The Use of the QuickVue Rapid Diagnostic Test. Journal of Medical Virology, 73(2), 269-273. https://doi.org/10.1002/jmv.20086

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title = "Influenza Virological Surveillance in Children: The Use of the QuickVue Rapid Diagnostic Test",

abstract = "New rapid diagnostic methods are needed to identify influenza infections to improve virological surveillance usually undertaken with conventional time-consuming, complex, and even expensive laboratory methods. Another reason for using a rapid test is to avoid inappropriate therapy, particularly in children, where use of antibiotics inappropriately and high influenza-related rates of hospitalisation are described. During two winter seasons, the performance of the QuickVue{\textregistered} Influenza test (QV) was evaluated in children under 14 presenting with influenza like illness, and compared the results with those obtained from sentinel network surveillance using standard protocols for the sample collection and the laboratory analysis by virus culture and reverse transcription-polymerase chain reaction (RT-PCR). During the first influenza season (2000/2001), 22 paediatricians collected one nose- and one throat-swab from each of the 586 children 0-6 years old recruited in the study. The QV test was carried out in the physician's office by primary care staff on the nose swab material. When compared with virus culture of the throat swab, the QV test had a sensitivity of 36.5%. In the following 2001/2002-influenza season, the performance of the QV test as a rapid laboratory screening assay was assessed. 342 children aged 0-14 years were enrolled with only one throat swab collected from each patient and sent to the laboratory where the QV, virus culture, and RT-PCR tests were performed. The results showed a better sensitivity (54.5%) of the test in comparison with virus culture and RT-PCR assays. The data indicate that rapid QV testing in the physician office setting, using these easily obtained samples, may be too insensitive to be useful for surveillance and for immediate clinical management of children presenting with influenza-like illness. Nevertheless, the QV test may be a valuable diagnostic tool if used in laboratory, as a rapid screening test.",

keywords = "Children, Diagnosis, Influenza virus, Polymerase chain reaction, Rapid test, Virus culture",

author = "Fabrizio Pregliasco and Simona Puzelli and Carolina Mensi and Giovanni Anselmi and Roberto Marinello and {Luisa Tanzi}, M. and Chiara Affinito and Maria Zambon and Isabella Donatelli and Francesco Argiro' and Emilio Bergonzi and Valeria Cammareri and Mauro Casati and Antonello Ciarroca and Salvatore Curto and Carlo Davoli and Giuseppe Elio and Concetta Fabiani and Renato Facconi and Giovanni Fasani and Francesca Giussani and Fabiola Frezza and Marco Giussani and Luigi Greco and Carlo Longhi and Nella Mezzabotta and Rinaldo Missaglia and Romualdo Moggio and Marco Montalbetti and Nino, {Luisa Maria} and Alberto Pedone and Marina Picca and Letizia Raimondi and Albino Ronchi and Rino Rosignoli and Luigi Siccardo and Giuseppe Varrasi and Guido Vertua",

year = "2004",

month = jun,

doi = "10.1002/jmv.20086",

language = "English",

volume = "73",

pages = "269--273",

journal = "Journal of Medical Virology",

issn = "0146-6615",

publisher = "John Wiley and Sons Inc.",

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Pregliasco, F, Puzelli, S, Mensi, C, Anselmi, G, Marinello, R, Luisa Tanzi, M, Affinito, C, Zambon, M, Donatelli, I, Argiro', F, Bergonzi, E, Cammareri, V, Casati, M, Ciarroca, A, Curto, S, Davoli, C, Elio, G, Fabiani, C, Facconi, R, Fasani, G, Giussani, F, Frezza, F, Giussani, M, Greco, L, Longhi, C, Mezzabotta, N, Missaglia, R, Moggio, R, Montalbetti, M, Nino, LM, Pedone, A, Picca, M, Raimondi, L, Ronchi, A, Rosignoli, R, Siccardo, L, Varrasi, G & Vertua, G 2004, 'Influenza Virological Surveillance in Children: The Use of the QuickVue Rapid Diagnostic Test', Journal of Medical Virology, vol. 73, no. 2, pp. 269-273. https://doi.org/10.1002/jmv.20086

TY - JOUR

T1 - Influenza Virological Surveillance in Children

T2 - The Use of the QuickVue Rapid Diagnostic Test

AU - Pregliasco, Fabrizio

AU - Puzelli, Simona

AU - Mensi, Carolina

AU - Anselmi, Giovanni

AU - Marinello, Roberto

AU - Luisa Tanzi, M.

AU - Affinito, Chiara

AU - Zambon, Maria

AU - Donatelli, Isabella

AU - Argiro', Francesco

AU - Bergonzi, Emilio

AU - Cammareri, Valeria

AU - Casati, Mauro

AU - Ciarroca, Antonello

AU - Curto, Salvatore

AU - Davoli, Carlo

AU - Elio, Giuseppe

AU - Fabiani, Concetta

AU - Facconi, Renato

AU - Fasani, Giovanni

AU - Giussani, Francesca

AU - Frezza, Fabiola

AU - Giussani, Marco

AU - Greco, Luigi

AU - Longhi, Carlo

AU - Mezzabotta, Nella

AU - Missaglia, Rinaldo

AU - Moggio, Romualdo

AU - Montalbetti, Marco

AU - Nino, Luisa Maria

AU - Pedone, Alberto

AU - Picca, Marina

AU - Raimondi, Letizia

AU - Ronchi, Albino

AU - Rosignoli, Rino

AU - Siccardo, Luigi

AU - Varrasi, Giuseppe

AU - Vertua, Guido

PY - 2004/6

Y1 - 2004/6

N2 - New rapid diagnostic methods are needed to identify influenza infections to improve virological surveillance usually undertaken with conventional time-consuming, complex, and even expensive laboratory methods. Another reason for using a rapid test is to avoid inappropriate therapy, particularly in children, where use of antibiotics inappropriately and high influenza-related rates of hospitalisation are described. During two winter seasons, the performance of the QuickVue® Influenza test (QV) was evaluated in children under 14 presenting with influenza like illness, and compared the results with those obtained from sentinel network surveillance using standard protocols for the sample collection and the laboratory analysis by virus culture and reverse transcription-polymerase chain reaction (RT-PCR). During the first influenza season (2000/2001), 22 paediatricians collected one nose- and one throat-swab from each of the 586 children 0-6 years old recruited in the study. The QV test was carried out in the physician's office by primary care staff on the nose swab material. When compared with virus culture of the throat swab, the QV test had a sensitivity of 36.5%. In the following 2001/2002-influenza season, the performance of the QV test as a rapid laboratory screening assay was assessed. 342 children aged 0-14 years were enrolled with only one throat swab collected from each patient and sent to the laboratory where the QV, virus culture, and RT-PCR tests were performed. The results showed a better sensitivity (54.5%) of the test in comparison with virus culture and RT-PCR assays. The data indicate that rapid QV testing in the physician office setting, using these easily obtained samples, may be too insensitive to be useful for surveillance and for immediate clinical management of children presenting with influenza-like illness. Nevertheless, the QV test may be a valuable diagnostic tool if used in laboratory, as a rapid screening test.

AB - New rapid diagnostic methods are needed to identify influenza infections to improve virological surveillance usually undertaken with conventional time-consuming, complex, and even expensive laboratory methods. Another reason for using a rapid test is to avoid inappropriate therapy, particularly in children, where use of antibiotics inappropriately and high influenza-related rates of hospitalisation are described. During two winter seasons, the performance of the QuickVue® Influenza test (QV) was evaluated in children under 14 presenting with influenza like illness, and compared the results with those obtained from sentinel network surveillance using standard protocols for the sample collection and the laboratory analysis by virus culture and reverse transcription-polymerase chain reaction (RT-PCR). During the first influenza season (2000/2001), 22 paediatricians collected one nose- and one throat-swab from each of the 586 children 0-6 years old recruited in the study. The QV test was carried out in the physician's office by primary care staff on the nose swab material. When compared with virus culture of the throat swab, the QV test had a sensitivity of 36.5%. In the following 2001/2002-influenza season, the performance of the QV test as a rapid laboratory screening assay was assessed. 342 children aged 0-14 years were enrolled with only one throat swab collected from each patient and sent to the laboratory where the QV, virus culture, and RT-PCR tests were performed. The results showed a better sensitivity (54.5%) of the test in comparison with virus culture and RT-PCR assays. The data indicate that rapid QV testing in the physician office setting, using these easily obtained samples, may be too insensitive to be useful for surveillance and for immediate clinical management of children presenting with influenza-like illness. Nevertheless, the QV test may be a valuable diagnostic tool if used in laboratory, as a rapid screening test.

KW - Children

KW - Diagnosis

KW - Influenza virus

KW - Polymerase chain reaction

KW - Rapid test

KW - Virus culture

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U2 - 10.1002/jmv.20086

DO - 10.1002/jmv.20086

M3 - Article

C2 - 15122803

AN - SCOPUS:11144357334

SN - 0146-6615

VL - 73

SP - 269

EP - 273

JO - Journal of Medical Virology

JF - Journal of Medical Virology

IS - 2

ER -

Influenza Virological Surveillance in Children: The Use of the QuickVue Rapid Diagnostic Test

Abstract

Keywords

UN SDGs

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