Improved multiplex PCR strategy for rapid assignment of the four major Escherichia coli phylogenetic groups

Michel Doumith; M. J. Day; Russell Hope; J. Wain; Neil Woodford

doi:10.1128/JCM.01468-12

Improved multiplex PCR strategy for rapid assignment of the four major Escherichia coli phylogenetic groups

Michel Doumith^*, M. J. Day, Russell Hope, J. Wain, Neil Woodford

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

152 Citations (Scopus)

Abstract

Using data from whole-genome projects, an updated multiplex PCR strategy was developed to assign Escherichia coli isolates rapidly to major phylogenetic groups. This assay accommodates sequence variations detected within target sequences, thereby increasing sensitivity and reliability. It was validated using 185 isolates of known sequence types and showed improved congruence with multilocus sequence typing data.

Original language	English
Pages (from-to)	3108-3110
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	50
Issue number	9
DOIs	https://doi.org/10.1128/JCM.01468-12
Publication status	Published - Sept 2012

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abstract = "Using data from whole-genome projects, an updated multiplex PCR strategy was developed to assign Escherichia coli isolates rapidly to major phylogenetic groups. This assay accommodates sequence variations detected within target sequences, thereby increasing sensitivity and reliability. It was validated using 185 isolates of known sequence types and showed improved congruence with multilocus sequence typing data.",

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Improved multiplex PCR strategy for rapid assignment of the four major Escherichia coli phylogenetic groups

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