Improved multiplex PCR strategy for rapid assignment of the four major Escherichia coli phylogenetic groups

Michel Doumith*, M. J. Day, Russell Hope, J. Wain, Neil Woodford

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

113 Citations (Scopus)

Abstract

Using data from whole-genome projects, an updated multiplex PCR strategy was developed to assign Escherichia coli isolates rapidly to major phylogenetic groups. This assay accommodates sequence variations detected within target sequences, thereby increasing sensitivity and reliability. It was validated using 185 isolates of known sequence types and showed improved congruence with multilocus sequence typing data.

Original languageEnglish
Pages (from-to)3108-3110
Number of pages3
JournalJournal of Clinical Microbiology
Volume50
Issue number9
DOIs
Publication statusPublished - Sep 2012

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