IgM antibody capture haemadherence test (MACHAT) for the detection of measles specific IgM

M. M. Siqueira*, Z. A. Ferro, B. J. Cohen, David Brown, J. P. Nascimento

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)

    Abstract

    An antibody capture haemadherence test (MACHAT) for detecting measles-specific IgM is described. The assay is based on the antibody capture principle with rhesus monkey erythrocytes as detector system in place of labelled antisera. MACHAT was compared with a commercial indirect enzyme immunoassay (EIA) for measles-specific IgM using 382 sera from patients notified as measles. There was good agreement between the two tests; 106 sera were found to contain measles IgM by both tests, 7 further sera were positive only in the commercial EIA and 9 only in MACHAT. One sera gave an equivocal result in MACHAT and another in the commercial EIA. Twelve of the 18 sera with discrepant results were also tested by MACRIA; in 7 MACRIA gave the same results as MACHAT, in 3 the MACRIA results agreed with the commercial test and in 2 the MACRIA results were equivocal. Specificity was established by a lack of MACHAT reactivity in sera collected from blood donors (n = 83) and from cases of recent rubella, dengue and parvovirus B19 infection (n = 51). The MACHAT is a simple, cheap test that can be read by eye and is suitable for measles surveillance programmes in the developing world.

    Original languageEnglish
    Pages (from-to)167-173
    Number of pages7
    JournalJournal of Virological Methods
    Volume50
    Issue number1-3
    DOIs
    Publication statusPublished - Dec 1994

    Bibliographical note

    Funding Information:
    This work was supported by the Ministry of Health, Brazil through a grant from COLAB/FNS/MS.B.J.C. and D.W.G.B. were visiting scientists to IOC/FIOCRUZ sponsoredb y CNPq, Brazil. The authors thank Dr. Otavio Oliva, from Biomanguinhos, for his critical reading of the manuscript.

    Copyright:
    Copyright 2018 Elsevier B.V., All rights reserved.

    Keywords

    • Haemadherence test
    • Measles
    • Rapid diagnosis

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