Identification of differentially expressed proteins from Burkholderia pseudomallei isolated during primary and relapsing melioidosis

Billie Velapatiño, Direk Limmathurotsakul, Sharon J. Peacock, David P. Speert*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Burkholderia pseudomallei causes septicemic melioidosis with a high rate of relapse, however microbial determinants of relapse are unknown. Proteins were analyzed from sequential B. pseudomallei isolates from primary and relapsing melioidosis. Analysis by isotope tagging for relative and absolute quantitation revealed that factors required for nitric oxide detoxification (HmpA) and necessary for anaerobic growth (ArcA, ArcC and ArcB) were highly expressed in the relapse isolate. Two-dimensional gel electrophoresis revealed up-regulation of a putative hemolysin-coregulated protein in the primary isolate, and flagellin and HSP20/alpha crystalline in the relapse isolate. These observations provide targets for further analysis of latency and virulence of melioidosis.

Original languageEnglish
Pages (from-to)335-340
Number of pages6
JournalMicrobes and Infection
Volume14
Issue number4
DOIs
Publication statusPublished - Apr 2012
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by a studentship from The University of British Columbia (to B. V.) and grants from Cystic Fibrosis Canada and Canadian Institutes of Health Research (to D.P.S.). D. L. is funded by the Wellcome Trust and Thailand Research Fund. S.J.P is funded by the Wellcome Trust.

Keywords

  • 2D-PAGE
  • Burkholderia pseudomallei
  • ITRAQ
  • Melioidosis
  • Proteomics

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