Identification Methods: DNA Fingerprinting: Pulsed-Field Gel Electrophoresis for Subtyping of Foodborne Pathogens

Tansy Peters; Ian Fisher

doi:10.1016/B978-0-12-384730-0.00409-2

Identification Methods: DNA Fingerprinting: Pulsed-Field Gel Electrophoresis for Subtyping of Foodborne Pathogens

Tansy Peters^*, Ian Fisher

^*Corresponding author for this work

Public Health England

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Pulsed-field gel electrophoresis (PFGE) was developed for separating and analyzing macrorestriction fragments of DNA in an alternating, homogenous electric field. Comparison of the electrophoretic banding profiles after digestion of intact bacterial DNA with restriction endonucleases is a useful epidemiological tool. Computer-assisted data analysis of PFGE subtypes has aided in many epidemiological studies and in tracing routes and sources of contamination in the food industry. For many foodborne disease infections, PFGE using a contour-clamped homogenous electric field (CHEF) is a still the gold standard for subtyping the implicated pathogens.

Original language	English
Title of host publication	Encyclopedia of Food Microbiology
Subtitle of host publication	Second Edition
Publisher	Elsevier Inc.
Pages	267-273
Number of pages	7
ISBN (Electronic)	9780123847331
ISBN (Print)	9780123847300
DOIs	https://doi.org/10.1016/B978-0-12-384730-0.00409-2
Publication status	Published - 2 Apr 2014

Keywords

Alternating electrical field
CHEF
Foodborne pathogens
Molecular epidemiology
PFGE

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10.1016/B978-0-12-384730-0.00409-2

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abstract = "Pulsed-field gel electrophoresis (PFGE) was developed for separating and analyzing macrorestriction fragments of DNA in an alternating, homogenous electric field. Comparison of the electrophoretic banding profiles after digestion of intact bacterial DNA with restriction endonucleases is a useful epidemiological tool. Computer-assisted data analysis of PFGE subtypes has aided in many epidemiological studies and in tracing routes and sources of contamination in the food industry. For many foodborne disease infections, PFGE using a contour-clamped homogenous electric field (CHEF) is a still the gold standard for subtyping the implicated pathogens.",

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AU - Fisher, Ian

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N2 - Pulsed-field gel electrophoresis (PFGE) was developed for separating and analyzing macrorestriction fragments of DNA in an alternating, homogenous electric field. Comparison of the electrophoretic banding profiles after digestion of intact bacterial DNA with restriction endonucleases is a useful epidemiological tool. Computer-assisted data analysis of PFGE subtypes has aided in many epidemiological studies and in tracing routes and sources of contamination in the food industry. For many foodborne disease infections, PFGE using a contour-clamped homogenous electric field (CHEF) is a still the gold standard for subtyping the implicated pathogens.

AB - Pulsed-field gel electrophoresis (PFGE) was developed for separating and analyzing macrorestriction fragments of DNA in an alternating, homogenous electric field. Comparison of the electrophoretic banding profiles after digestion of intact bacterial DNA with restriction endonucleases is a useful epidemiological tool. Computer-assisted data analysis of PFGE subtypes has aided in many epidemiological studies and in tracing routes and sources of contamination in the food industry. For many foodborne disease infections, PFGE using a contour-clamped homogenous electric field (CHEF) is a still the gold standard for subtyping the implicated pathogens.

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KW - CHEF

KW - Foodborne pathogens

KW - Molecular epidemiology

KW - PFGE

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SN - 9780123847300

SP - 267

EP - 273

BT - Encyclopedia of Food Microbiology

PB - Elsevier Inc.

ER -

Identification Methods: DNA Fingerprinting: Pulsed-Field Gel Electrophoresis for Subtyping of Foodborne Pathogens

Abstract

Keywords

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