Identification by genomic immunization of a pool of DNA vaccine candidates that confer protective immunity in mice against Neisseria meningitidis serogroup B

Daniel Yero; Rolando Pajón; Yusleydis Pérez; Mildrey Fariñas; Karem Cobas; Daiyana Diaz; Rosa L. Solis; Armando Acosta; Charlotte Brookes; Stephen Taylor; Andrew Gorringe

doi:10.1016/j.vaccine.2007.04.084

Identification by genomic immunization of a pool of DNA vaccine candidates that confer protective immunity in mice against Neisseria meningitidis serogroup B

Daniel Yero, Rolando Pajón, Yusleydis Pérez, Mildrey Fariñas, Karem Cobas, Daiyana Diaz, Rosa L. Solis, Armando Acosta, Charlotte Brookes, Stephen Taylor, Andrew Gorringe^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

We have shown previously that expression library immunization is viable alternative approach to induce protective immunity against Neisseria meningitidis serogroup B. In this study we report that few rounds of library screening allow identification of protective pools of defined antigens. A previously reported protective meningococcal library (L8, with 600 clones) was screened and two sub-libraries of 95 clones each were selected based on the induction of bactericidal and protective antibodies in BALB/c mice. After sequence analysis of each clone within these sub-libraries, we identified a pool of 20 individual antigens that induced protective immune responses in mice against N. meningitidis infection, and the observed protection was associated with the induction of bactericidal antibodies. Our studies demonstrate for the first time that ELI combined with sequence analysis is a powerful and efficient tool for identification of candidate antigens for use in a meningococcal vaccine.

Original language	English
Pages (from-to)	5175-5188
Number of pages	14
Journal	Vaccine
Volume	25
Issue number	28
DOIs	https://doi.org/10.1016/j.vaccine.2007.04.084
Publication status	Published - 9 Jul 2007

Bibliographical note

Funding Information:
We are grateful to Sergio Sifontes at Finlay Institute for providing facilities for experimental work with animals, especially on the establishment of the mice protection model. Research at the Health Protection Agency was funded by the UK Department of Health.

Keywords

Expression library
Genomic immunization
Neisseria meningitidis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.vaccine.2007.04.084

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Yero, D., Pajón, R., Pérez, Y., Fariñas, M., Cobas, K., Diaz, D., Solis, R. L., Acosta, A., Brookes, C., Taylor, S., & Gorringe, A. (2007). Identification by genomic immunization of a pool of DNA vaccine candidates that confer protective immunity in mice against Neisseria meningitidis serogroup B. Vaccine, 25(28), 5175-5188. https://doi.org/10.1016/j.vaccine.2007.04.084

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title = "Identification by genomic immunization of a pool of DNA vaccine candidates that confer protective immunity in mice against Neisseria meningitidis serogroup B",

abstract = "We have shown previously that expression library immunization is viable alternative approach to induce protective immunity against Neisseria meningitidis serogroup B. In this study we report that few rounds of library screening allow identification of protective pools of defined antigens. A previously reported protective meningococcal library (L8, with 600 clones) was screened and two sub-libraries of 95 clones each were selected based on the induction of bactericidal and protective antibodies in BALB/c mice. After sequence analysis of each clone within these sub-libraries, we identified a pool of 20 individual antigens that induced protective immune responses in mice against N. meningitidis infection, and the observed protection was associated with the induction of bactericidal antibodies. Our studies demonstrate for the first time that ELI combined with sequence analysis is a powerful and efficient tool for identification of candidate antigens for use in a meningococcal vaccine.",

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Yero, D, Pajón, R, Pérez, Y, Fariñas, M, Cobas, K, Diaz, D, Solis, RL, Acosta, A, Brookes, C , Taylor, S & Gorringe, A 2007, 'Identification by genomic immunization of a pool of DNA vaccine candidates that confer protective immunity in mice against Neisseria meningitidis serogroup B', Vaccine, vol. 25, no. 28, pp. 5175-5188. https://doi.org/10.1016/j.vaccine.2007.04.084

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AU - Yero, Daniel

AU - Pajón, Rolando

AU - Pérez, Yusleydis

AU - Fariñas, Mildrey

AU - Cobas, Karem

AU - Diaz, Daiyana

AU - Solis, Rosa L.

AU - Acosta, Armando

AU - Brookes, Charlotte

AU - Taylor, Stephen

AU - Gorringe, Andrew

N1 - Funding Information: We are grateful to Sergio Sifontes at Finlay Institute for providing facilities for experimental work with animals, especially on the establishment of the mice protection model. Research at the Health Protection Agency was funded by the UK Department of Health.

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AB - We have shown previously that expression library immunization is viable alternative approach to induce protective immunity against Neisseria meningitidis serogroup B. In this study we report that few rounds of library screening allow identification of protective pools of defined antigens. A previously reported protective meningococcal library (L8, with 600 clones) was screened and two sub-libraries of 95 clones each were selected based on the induction of bactericidal and protective antibodies in BALB/c mice. After sequence analysis of each clone within these sub-libraries, we identified a pool of 20 individual antigens that induced protective immune responses in mice against N. meningitidis infection, and the observed protection was associated with the induction of bactericidal antibodies. Our studies demonstrate for the first time that ELI combined with sequence analysis is a powerful and efficient tool for identification of candidate antigens for use in a meningococcal vaccine.

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Identification by genomic immunization of a pool of DNA vaccine candidates that confer protective immunity in mice against Neisseria meningitidis serogroup B

Abstract

Bibliographical note

Keywords

UN SDGs

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