Highly specific gene silencing by artificial microRNAs in the unicellular alga Chlamydomonas reinhardtii: TECHNICAL ADVANCE

Attila Molnar, Andrew Bassett, Eva Thuenemann, Frank Schwach, Shantanu Karkare, Stephan Ossowski, Detlef Weigel, David Baulcombe*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

259 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) are small RNAs, 21 to 22 nucleotides long, with important regulatory roles. They are processed from longer RNA molecules with imperfectly matched foldback regions and they function in modulating the stability and translation of mRNA. Recently, we and others have demonstrated that the unicellular alga Chlamydomonas reinhardtii, like diverse multicellular organisms, contains miRNAs. These RNAs resemble the miRNAs of land plants in that they direct site-specific cleavage of target mRNA with miRNA-complementary motifs and, presumably, act as regulatory molecules in growth and development. Utilizing these findings we have developed a novel artificial miRNA system based on ligation of DNA oligonucleotides that can be used for specific high-throughput gene silencing in green algae.

Original languageEnglish
Pages (from-to)165-174
Number of pages10
JournalPlant Journal
Volume58
Issue number1
DOIs
Publication statusPublished - Apr 2009
Externally publishedYes

Keywords

  • Artificial miRNA
  • Chlamydomonas
  • Functional genomics
  • Oligonucleotide cloning
  • RNA silencing

Fingerprint

Dive into the research topics of 'Highly specific gene silencing by artificial microRNAs in the unicellular alga Chlamydomonas reinhardtii: TECHNICAL ADVANCE'. Together they form a unique fingerprint.

Cite this