High-throughput whole-genome sequencing to dissect the epidemiology of Acinetobacter baumannii isolates from a hospital outbreak

T. Lewis; N. J. Loman; L. Bingle; P. Jumaa; G. M. Weinstock; D. Mortiboy; M. J. Pallen

doi:10.1016/j.jhin.2010.01.012

High-throughput whole-genome sequencing to dissect the epidemiology of Acinetobacter baumannii isolates from a hospital outbreak

T. Lewis, N. J. Loman, L. Bingle, P. Jumaa, G. M. Weinstock, D. Mortiboy, M. J. Pallen^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

131 Citations (Scopus)

Abstract

Shared care of military and civilian patients has resulted in transmission of multidrug-resistant Acinetobacter baumannii (MDR-Aci) from military casualties to civilians. Current typing technologies have been useful in revealing relationships between isolates of A. baumannii but they are unable to resolve differences between closely related isolates from small-scale outbreaks, where chains of transmission are often unclear. In a recent hospital outbreak in Birmingham, six patients were colonised with MDR-Aci isolates indistinguishable using standard techniques. We used whole-genome sequencing to identify single nucleotide polymorphisms in these isolates, allowing us to discriminate between alternative epidemiological hypotheses in this setting.

Original language	English
Pages (from-to)	37-41
Number of pages	5
Journal	Journal of Hospital Infection
Volume	75
Issue number	1
DOIs	https://doi.org/10.1016/j.jhin.2010.01.012
Publication status	Published - May 2010
Externally published	Yes

Bibliographical note

Funding Information:
Genome sequencing was funded by a Small Research Grant from the Hospital Infection Society, London, UK. The xBASE facility and Loman's position are funded by BBSRC grant BBE0111791 .

Keywords

454 pyrosequencing
Acinetobacter baumannii
Epidemiology
Multidrug resistance
Single nucleotide polymorphism
Whole-genome sequencing

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jhin.2010.01.012

Cite this

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title = "High-throughput whole-genome sequencing to dissect the epidemiology of Acinetobacter baumannii isolates from a hospital outbreak",

abstract = "Shared care of military and civilian patients has resulted in transmission of multidrug-resistant Acinetobacter baumannii (MDR-Aci) from military casualties to civilians. Current typing technologies have been useful in revealing relationships between isolates of A. baumannii but they are unable to resolve differences between closely related isolates from small-scale outbreaks, where chains of transmission are often unclear. In a recent hospital outbreak in Birmingham, six patients were colonised with MDR-Aci isolates indistinguishable using standard techniques. We used whole-genome sequencing to identify single nucleotide polymorphisms in these isolates, allowing us to discriminate between alternative epidemiological hypotheses in this setting.",

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note = "Funding Information: Genome sequencing was funded by a Small Research Grant from the Hospital Infection Society, London, UK. The xBASE facility and Loman's position are funded by BBSRC grant BBE0111791 .",

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AU - Lewis, T.

AU - Loman, N. J.

AU - Bingle, L.

AU - Jumaa, P.

AU - Weinstock, G. M.

AU - Mortiboy, D.

AU - Pallen, M. J.

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AB - Shared care of military and civilian patients has resulted in transmission of multidrug-resistant Acinetobacter baumannii (MDR-Aci) from military casualties to civilians. Current typing technologies have been useful in revealing relationships between isolates of A. baumannii but they are unable to resolve differences between closely related isolates from small-scale outbreaks, where chains of transmission are often unclear. In a recent hospital outbreak in Birmingham, six patients were colonised with MDR-Aci isolates indistinguishable using standard techniques. We used whole-genome sequencing to identify single nucleotide polymorphisms in these isolates, allowing us to discriminate between alternative epidemiological hypotheses in this setting.

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KW - Epidemiology

KW - Multidrug resistance

KW - Single nucleotide polymorphism

KW - Whole-genome sequencing

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High-throughput whole-genome sequencing to dissect the epidemiology of Acinetobacter baumannii isolates from a hospital outbreak

Abstract

Bibliographical note

Keywords

UN SDGs

Access to Document

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