High-throughput whole-genome sequencing to dissect the epidemiology of Acinetobacter baumannii isolates from a hospital outbreak

T. Lewis, N. J. Loman, L. Bingle, P. Jumaa, G. M. Weinstock, D. Mortiboy, M. J. Pallen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

110 Citations (Scopus)

Abstract

Shared care of military and civilian patients has resulted in transmission of multidrug-resistant Acinetobacter baumannii (MDR-Aci) from military casualties to civilians. Current typing technologies have been useful in revealing relationships between isolates of A. baumannii but they are unable to resolve differences between closely related isolates from small-scale outbreaks, where chains of transmission are often unclear. In a recent hospital outbreak in Birmingham, six patients were colonised with MDR-Aci isolates indistinguishable using standard techniques. We used whole-genome sequencing to identify single nucleotide polymorphisms in these isolates, allowing us to discriminate between alternative epidemiological hypotheses in this setting.

Original languageEnglish
Pages (from-to)37-41
Number of pages5
JournalJournal of Hospital Infection
Volume75
Issue number1
DOIs
Publication statusPublished - May 2010
Externally publishedYes

Bibliographical note

Funding Information:
Genome sequencing was funded by a Small Research Grant from the Hospital Infection Society, London, UK. The xBASE facility and Loman's position are funded by BBSRC grant BBE0111791 .

Keywords

  • 454 pyrosequencing
  • Acinetobacter baumannii
  • Epidemiology
  • Multidrug resistance
  • Single nucleotide polymorphism
  • Whole-genome sequencing

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